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Bacterial diversity and reductive dehalogenase redundancy in a 1,2-dichloroethane-degrading bacterial consortium enriched from a contaminated aquifer

Massimo Marzorati UGent, Annalisa Balloi, Francesca de Ferra, Lorenzo Corallo, Giovanna Carpani, Lieven Wittebolle UGent, Willy Verstraete and Daniele Daffonchio (2010) MICROBIAL CELL FACTORIES. 9.
abstract
Background: Bacteria possess a reservoir of metabolic functionalities ready to be exploited for multiple purposes. The use of microorganisms to clean up xenobiotics from polluted ecosystems (e. g. soil and water) represents an eco-sustainable and powerful alternative to traditional remediation processes. Recent developments in molecular-biology-based techniques have led to rapid and accurate strategies for monitoring and identification of bacteria and catabolic genes involved in the degradation of xenobiotics, key processes to follow up the activities in situ. Results: We report the characterization of the response of an enriched bacterial community of a 1,2-dichloroethane (1,2-DCA) contaminated aquifer to the spiking with 5 mM lactate as electron donor in microcosm studies. After 15 days of incubation, the microbial community structure was analyzed. The bacterial 16S rRNA gene clone library showed that the most represented phylogenetic group within the consortium was affiliated with the phylum Firmicutes. Among them, known degraders of chlorinated compounds were identified. A reductive dehalogenase genes clone library showed that the community held four phylogenetically-distinct catalytic enzymes, all conserving signature residues previously shown to be linked to 1,2-DCA dehalogenation. Conclusions: The overall data indicate that the enriched bacterial consortium shares the metabolic functionality between different members of the microbial community and is characterized by a high functional redundancy. These are fundamental features for the maintenance of the community's functionality, especially under stress conditions and suggest the feasibility of a bioremediation treatment with a potential prompt dehalogenation and a process stability over time.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
GEN. NOV., MOLECULAR CHARACTERIZATION, MICROBIAL ECOLOGY, MULTIPLE SEQUENCE ALIGNMENT, DICHLOROELIMINANS STRAIN DCA1, SITU BIOSTIMULATION, GROUNDWATER, DECHLORINATION, TETRACHLOROETHENE, BIOAUGMENTATION
journal title
MICROBIAL CELL FACTORIES
Microb. Cell. Fact.
volume
9
article number
12
pages
9 pages
Web of Science type
Article
Web of Science id
000275465500001
JCR category
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
JCR impact factor
4.544 (2010)
JCR rank
21/158 (2010)
JCR quartile
1 (2010)
ISSN
1475-2859
DOI
10.1186/1475-2859-9-12
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
1086650
handle
http://hdl.handle.net/1854/LU-1086650
date created
2010-12-13 16:03:47
date last changed
2016-12-21 15:42:24
@article{1086650,
  abstract     = {Background: Bacteria possess a reservoir of metabolic functionalities ready to be exploited for multiple purposes. The use of microorganisms to clean up xenobiotics from polluted ecosystems (e. g. soil and water) represents an eco-sustainable and powerful alternative to traditional remediation processes. Recent developments in molecular-biology-based techniques have led to rapid and accurate strategies for monitoring and identification of bacteria and catabolic genes involved in the degradation of xenobiotics, key processes to follow up the activities in situ.
Results: We report the characterization of the response of an enriched bacterial community of a 1,2-dichloroethane (1,2-DCA) contaminated aquifer to the spiking with 5 mM lactate as electron donor in microcosm studies. After 15 days of incubation, the microbial community structure was analyzed. The bacterial 16S rRNA gene clone library showed that the most represented phylogenetic group within the consortium was affiliated with the phylum Firmicutes. Among them, known degraders of chlorinated compounds were identified. A reductive dehalogenase genes clone library showed that the community held four phylogenetically-distinct catalytic enzymes, all conserving signature residues previously shown to be linked to 1,2-DCA dehalogenation.
Conclusions: The overall data indicate that the enriched bacterial consortium shares the metabolic functionality between different members of the microbial community and is characterized by a high functional redundancy. These are fundamental features for the maintenance of the community's functionality, especially under stress conditions and suggest the feasibility of a bioremediation treatment with a potential prompt dehalogenation and a process stability over time.},
  articleno    = {12},
  author       = {Marzorati, Massimo and Balloi, Annalisa and de Ferra, Francesca and Corallo, Lorenzo and Carpani, Giovanna and Wittebolle, Lieven and Verstraete, Willy and Daffonchio, Daniele},
  issn         = {1475-2859},
  journal      = {MICROBIAL CELL FACTORIES},
  keyword      = {GEN. NOV.,MOLECULAR CHARACTERIZATION,MICROBIAL ECOLOGY,MULTIPLE SEQUENCE ALIGNMENT,DICHLOROELIMINANS STRAIN DCA1,SITU BIOSTIMULATION,GROUNDWATER,DECHLORINATION,TETRACHLOROETHENE,BIOAUGMENTATION},
  language     = {eng},
  pages        = {9},
  title        = {Bacterial diversity and reductive dehalogenase redundancy in a 1,2-dichloroethane-degrading bacterial consortium enriched from a contaminated aquifer},
  url          = {http://dx.doi.org/10.1186/1475-2859-9-12},
  volume       = {9},
  year         = {2010},
}

Chicago
Marzorati, Massimo, Annalisa Balloi, Francesca de Ferra, Lorenzo Corallo, Giovanna Carpani, Lieven Wittebolle, Willy Verstraete, and Daniele Daffonchio. 2010. “Bacterial Diversity and Reductive Dehalogenase Redundancy in a 1,2-dichloroethane-degrading Bacterial Consortium Enriched from a Contaminated Aquifer.” Microbial Cell Factories 9.
APA
Marzorati, M., Balloi, A., de Ferra, F., Corallo, L., Carpani, G., Wittebolle, L., Verstraete, W., et al. (2010). Bacterial diversity and reductive dehalogenase redundancy in a 1,2-dichloroethane-degrading bacterial consortium enriched from a contaminated aquifer. MICROBIAL CELL FACTORIES, 9.
Vancouver
1.
Marzorati M, Balloi A, de Ferra F, Corallo L, Carpani G, Wittebolle L, et al. Bacterial diversity and reductive dehalogenase redundancy in a 1,2-dichloroethane-degrading bacterial consortium enriched from a contaminated aquifer. MICROBIAL CELL FACTORIES. 2010;9.
MLA
Marzorati, Massimo, Annalisa Balloi, Francesca de Ferra, et al. “Bacterial Diversity and Reductive Dehalogenase Redundancy in a 1,2-dichloroethane-degrading Bacterial Consortium Enriched from a Contaminated Aquifer.” MICROBIAL CELL FACTORIES 9 (2010): n. pag. Print.