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Genotypic and phenotypic characterization of the European A2 isolates of Phytophthora ramorum

Annelies Vercauteren UGent, Isabelle De Dobbelaere UGent, Erik Van Bockstaele UGent, Martine Maes and Kurt Heungens (2011) EUROPEAN JOURNAL OF PLANT PATHOLOGY. 129(4). p.621-635
abstract
Phytophthora ramorum, a recently described North American and European pathogen, has three clonal lineages. The NA1 and NA2 lineages are found in North American forests and nurseries, while the EU1 lineage appears mainly in European nurseries. P. ramorum is heterothallic, having two mating types A1 and A2. All NA1 and NA2 isolates are of A2 mating type. When first collected, all EU1 isolates were of A1 mating type, with the exception of one A2 isolate collected in Belgium in 2002. Screening 410 other Belgian isolates for mating type revealed two additional EU1-A2 isolates collected in 2002 and 2003. PCR-RFLP, AFLP and SSR markers were used to determine the nature of the mating type change. The three isolates show no indications of sexual recombination or mitotic crossing over, indicating that mutation or mitotic gene conversion is the most likely explanation for the mating type change. We compared the pathogenicity and sporulation characteristics of the EU1-A2 isolates to those of EU1-A1 and NA1-A2 isolates on four host plants. Despite small differences in pathogenicity on some hosts, the EU1-A2 isolates were similarly aggressive to each other and to the EU1-A1 isolates and more aggressive than the NA1-A2 isolates. Sporulation characteristics were also comparable among EU1-A2 isolates and between EU1-A1 and EU1-A2 isolates, except for EU1-A2 isolate BBA 26/02. The limited genotypic and phenotypic differences between EU1-A2 isolates probably evolved after the mating type change, which may have occurred several years before the isolates were detected. There are strong indications that the EU1-A2 population has been eradicated from Belgium.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Aggressiveness, Mating type, Oomycetes, Sporulation, Sudden Oak Death, SUDDEN OAK DEATH, IN-VITRO, NORTH-AMERICAN, MATING-TYPE, PATHOGEN, SUSCEPTIBILITY, CALIFORNIA, RHODODENDRON, POPULATIONS, VIBURNUM
journal title
EUROPEAN JOURNAL OF PLANT PATHOLOGY
Eur. J. Plant Pathol.
volume
129
issue
4
pages
621 - 635
Web of Science type
Article
Web of Science id
000287967400011
JCR category
HORTICULTURE
JCR impact factor
1.413 (2011)
JCR rank
9/31 (2011)
JCR quartile
2 (2011)
ISSN
0929-1873
DOI
10.1007/s10658-010-9727-5
project
Biotechnology for a sustainable economy (Bio-Economy)
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1086258
handle
http://hdl.handle.net/1854/LU-1086258
date created
2010-12-11 13:29:49
date last changed
2013-02-27 09:35:00
@article{1086258,
  abstract     = {Phytophthora ramorum, a recently described North American and European pathogen, has three clonal lineages. The NA1 and NA2 lineages are found in North American forests and nurseries, while the EU1 lineage appears mainly in European nurseries. P. ramorum is heterothallic, having two mating types A1 and A2. All NA1 and NA2 isolates are of A2 mating type. When first collected, all EU1 isolates were of A1 mating type, with the exception of one A2 isolate collected in Belgium in 2002. Screening 410 other Belgian isolates for mating type revealed two additional EU1-A2 isolates collected in 2002 and 2003. PCR-RFLP, AFLP and SSR markers were used to determine the nature of the mating type change. The three isolates show no indications of sexual recombination or mitotic crossing over, indicating that mutation or mitotic gene conversion is the most likely explanation for the mating type change. We compared the pathogenicity and sporulation characteristics of the EU1-A2 isolates to those of EU1-A1 and NA1-A2 isolates on four host plants. Despite small differences in pathogenicity on some hosts, the EU1-A2 isolates were similarly aggressive to each other and to the EU1-A1 isolates and more aggressive than the NA1-A2 isolates. Sporulation characteristics were also comparable among EU1-A2 isolates and between EU1-A1 and EU1-A2 isolates, except for EU1-A2 isolate BBA 26/02. The limited genotypic and phenotypic differences between EU1-A2 isolates probably evolved after the mating type change, which may have occurred several years before the isolates were detected. There are strong indications that the EU1-A2 population has been eradicated from Belgium.},
  author       = {Vercauteren, Annelies and De Dobbelaere, Isabelle and Van Bockstaele, Erik and Maes, Martine and Heungens, Kurt},
  issn         = {0929-1873},
  journal      = {EUROPEAN JOURNAL OF PLANT PATHOLOGY},
  keyword      = {Aggressiveness,Mating type,Oomycetes,Sporulation,Sudden Oak Death,SUDDEN OAK DEATH,IN-VITRO,NORTH-AMERICAN,MATING-TYPE,PATHOGEN,SUSCEPTIBILITY,CALIFORNIA,RHODODENDRON,POPULATIONS,VIBURNUM},
  language     = {eng},
  number       = {4},
  pages        = {621--635},
  title        = {Genotypic and phenotypic characterization of the European A2 isolates of Phytophthora ramorum},
  url          = {http://dx.doi.org/10.1007/s10658-010-9727-5},
  volume       = {129},
  year         = {2011},
}

Chicago
Vercauteren, Annelies, Isabelle De Dobbelaere, Erik Van Bockstaele, Martine Maes, and Kurt Heungens. 2011. “Genotypic and Phenotypic Characterization of the European A2 Isolates of Phytophthora Ramorum.” European Journal of Plant Pathology 129 (4): 621–635.
APA
Vercauteren, Annelies, De Dobbelaere, I., Van Bockstaele, E., Maes, M., & Heungens, K. (2011). Genotypic and phenotypic characterization of the European A2 isolates of Phytophthora ramorum. EUROPEAN JOURNAL OF PLANT PATHOLOGY, 129(4), 621–635.
Vancouver
1.
Vercauteren A, De Dobbelaere I, Van Bockstaele E, Maes M, Heungens K. Genotypic and phenotypic characterization of the European A2 isolates of Phytophthora ramorum. EUROPEAN JOURNAL OF PLANT PATHOLOGY. 2011;129(4):621–35.
MLA
Vercauteren, Annelies, Isabelle De Dobbelaere, Erik Van Bockstaele, et al. “Genotypic and Phenotypic Characterization of the European A2 Isolates of Phytophthora Ramorum.” EUROPEAN JOURNAL OF PLANT PATHOLOGY 129.4 (2011): 621–635. Print.