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Complete nucleotide sequence of CTX-M-15-plasmids from clinical Escherichia coli isolates: insertional events of transposons and insertion sequences

Annemieke Smet UGent, Filip Van Nieuwerburgh UGent, Tom Vandekerckhove UGent, An Martel UGent, Dieter Deforce UGent, Patrick Butaye UGent and Freddy Haesebrouck UGent (2010) PLOS ONE. 5(6).
abstract
Background: CTX-M-producing Escherichia coli strains are regarded as major global pathogens. Methodology/Principal Findings: The nucleotide sequence of three plasmids (pEC_B24: 73801-bp; pEC_L8: 118525-bp and pEC_L46: 144871-bp) from Escherichia coli isolates obtained from patients with urinary tract infections and one plasmid (pEC_Bactec: 92970-bp) from an Escherichia coli strain isolated from the joint of a horse with arthritis were determined. Plasmid pEC_Bactec belongs to the Incl1 group and carries two resistance genes: bla(TEM-1) and bla(CTX-M-15). It shares more than 90% homology with a previously published bla(CTX-M)-plasmid from E. coli of human origin. Plasmid pEC_B24 belongs to the IncFII group whereas plasmids pEC_L8 and pEC_L46 represent a fusion of two replicons of type FII and FIA. On the pEC_B24 backbone, two resistance genes, blaTEM-1 and blaCTX-M-15, were found. Six resistance genes, blaTEM-1, blaCTX-M-15, bla(OXA-1), aac6'-lb-cr, tetA and catB4, were detected on the pEC_L8 backbone. The same antimicrobial drug resistance genes, with the exception of tetA, were also identified on the pEC_L46 backbone. Genome analysis of all 4 plasmids studied provides evidence of a seemingly frequent transposition event of the bla(CTX-M-15)-ISEcp1 element. This element seems to have a preferred insertion site at the tnpA gene of a bla(TEM)-carrying Tn3-like transposon, the latter itself being inserted by a transposition event. The IS26-composite transposon, which contains the blaOXA-1, aac6'-lb-cr and catB4 genes, was inserted into plasmids pEC_L8 and pEC_L46 by homologous recombination rather than a transposition event. Results obtained for pEC_L46 indicated that IS26 also plays an important role in structural rearrangements of the plasmid backbone and seems to facilitate the mobilisation of fragments from other plasmids. Conclusions: Collectively, these data suggests that IS26 together with ISEcp1 could play a critical role in the evolution of diverse multiresistant plasmids found in clinical Enterobacteriaceae.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
PLASMID, GENES, RESISTANCE, CTX-M, DNA-SEQUENCE, SPECTRUM-BETA-LACTAMASE, MESSENGER-RNA, ENTEROBACTERIACEAE, DISSEMINATION, SALMONELLA
journal title
PLOS ONE
PLoS One
volume
5
issue
6
article_number
e11202
pages
8 pages
Web of Science type
Article
Web of Science id
000278977200007
JCR category
BIOLOGY
JCR impact factor
4.411 (2010)
JCR rank
12/84 (2010)
JCR quartile
1 (2010)
ISSN
1932-6203
DOI
10.1371/journal.pone.0011202
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
1086016
handle
http://hdl.handle.net/1854/LU-1086016
date created
2010-12-10 10:05:43
date last changed
2010-12-10 12:08:26
@article{1086016,
  abstract     = {Background: CTX-M-producing Escherichia coli strains are regarded as major global pathogens.
Methodology/Principal Findings: The nucleotide sequence of three plasmids (pEC\_B24: 73801-bp; pEC\_L8: 118525-bp and pEC\_L46: 144871-bp) from Escherichia coli isolates obtained from patients with urinary tract infections and one plasmid (pEC\_Bactec: 92970-bp) from an Escherichia coli strain isolated from the joint of a horse with arthritis were determined. Plasmid pEC\_Bactec belongs to the Incl1 group and carries two resistance genes: bla(TEM-1) and bla(CTX-M-15). It shares more than 90\% homology with a previously published bla(CTX-M)-plasmid from E. coli of human origin. Plasmid pEC\_B24 belongs to the IncFII group whereas plasmids pEC\_L8 and pEC\_L46 represent a fusion of two replicons of type FII and FIA. On the pEC\_B24 backbone, two resistance genes, blaTEM-1 and blaCTX-M-15, were found. Six resistance genes, blaTEM-1, blaCTX-M-15, bla(OXA-1), aac6'-lb-cr, tetA and catB4, were detected on the pEC\_L8 backbone. The same antimicrobial drug resistance genes, with the exception of tetA, were also identified on the pEC\_L46 backbone. Genome analysis of all 4 plasmids studied provides evidence of a seemingly frequent transposition event of the bla(CTX-M-15)-ISEcp1 element. This element seems to have a preferred insertion site at the tnpA gene of a bla(TEM)-carrying Tn3-like transposon, the latter itself being inserted by a transposition event. The IS26-composite transposon, which contains the blaOXA-1, aac6'-lb-cr and catB4 genes, was inserted into plasmids pEC\_L8 and pEC\_L46 by homologous recombination rather than a transposition event. Results obtained for pEC\_L46 indicated that IS26 also plays an important role in structural rearrangements of the plasmid backbone and seems to facilitate the mobilisation of fragments from other plasmids.
Conclusions: Collectively, these data suggests that IS26 together with ISEcp1 could play a critical role in the evolution of diverse multiresistant plasmids found in clinical Enterobacteriaceae.},
  articleno    = {e11202},
  author       = {Smet, Annemieke and Van Nieuwerburgh, Filip and Vandekerckhove, Tom and Martel, An and Deforce, Dieter and Butaye, Patrick and Haesebrouck, Freddy},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  keyword      = {PLASMID,GENES,RESISTANCE,CTX-M,DNA-SEQUENCE,SPECTRUM-BETA-LACTAMASE,MESSENGER-RNA,ENTEROBACTERIACEAE,DISSEMINATION,SALMONELLA},
  language     = {eng},
  number       = {6},
  pages        = {8},
  title        = {Complete nucleotide sequence of CTX-M-15-plasmids from clinical Escherichia coli isolates: insertional events of transposons and insertion sequences},
  url          = {http://dx.doi.org/10.1371/journal.pone.0011202},
  volume       = {5},
  year         = {2010},
}

Chicago
Smet, Annemieke, Filip Van Nieuwerburgh, Tom Vandekerckhove, An Martel, Dieter Deforce, Patrick Butaye, and Freddy Haesebrouck. 2010. “Complete Nucleotide Sequence of CTX-M-15-plasmids from Clinical Escherichia Coli Isolates: Insertional Events of Transposons and Insertion Sequences.” Plos One 5 (6).
APA
Smet, A., Van Nieuwerburgh, F., Vandekerckhove, T., Martel, A., Deforce, D., Butaye, P., & Haesebrouck, F. (2010). Complete nucleotide sequence of CTX-M-15-plasmids from clinical Escherichia coli isolates: insertional events of transposons and insertion sequences. PLOS ONE, 5(6).
Vancouver
1.
Smet A, Van Nieuwerburgh F, Vandekerckhove T, Martel A, Deforce D, Butaye P, et al. Complete nucleotide sequence of CTX-M-15-plasmids from clinical Escherichia coli isolates: insertional events of transposons and insertion sequences. PLOS ONE. 2010;5(6).
MLA
Smet, Annemieke, Filip Van Nieuwerburgh, Tom Vandekerckhove, et al. “Complete Nucleotide Sequence of CTX-M-15-plasmids from Clinical Escherichia Coli Isolates: Insertional Events of Transposons and Insertion Sequences.” PLOS ONE 5.6 (2010): n. pag. Print.