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Complete nucleotide sequence of CTX-M-15-plasmids from clinical Escherichia coli isolates: insertional events of transposons and insertion sequences

Annemieke Smet (UGent) , Filip Van Nieuwerburgh (UGent) , Tom Vandekerckhove (UGent) , An Martel (UGent) , Dieter Deforce (UGent) , Patrick Butaye (UGent) and Freddy Haesebrouck (UGent)
(2010) PLOS ONE. 5(6).
Author
Organization
Abstract
Background: CTX-M-producing Escherichia coli strains are regarded as major global pathogens. Methodology/Principal Findings: The nucleotide sequence of three plasmids (pEC_B24: 73801-bp; pEC_L8: 118525-bp and pEC_L46: 144871-bp) from Escherichia coli isolates obtained from patients with urinary tract infections and one plasmid (pEC_Bactec: 92970-bp) from an Escherichia coli strain isolated from the joint of a horse with arthritis were determined. Plasmid pEC_Bactec belongs to the Incl1 group and carries two resistance genes: bla(TEM-1) and bla(CTX-M-15). It shares more than 90% homology with a previously published bla(CTX-M)-plasmid from E. coli of human origin. Plasmid pEC_B24 belongs to the IncFII group whereas plasmids pEC_L8 and pEC_L46 represent a fusion of two replicons of type FII and FIA. On the pEC_B24 backbone, two resistance genes, blaTEM-1 and blaCTX-M-15, were found. Six resistance genes, blaTEM-1, blaCTX-M-15, bla(OXA-1), aac6'-lb-cr, tetA and catB4, were detected on the pEC_L8 backbone. The same antimicrobial drug resistance genes, with the exception of tetA, were also identified on the pEC_L46 backbone. Genome analysis of all 4 plasmids studied provides evidence of a seemingly frequent transposition event of the bla(CTX-M-15)-ISEcp1 element. This element seems to have a preferred insertion site at the tnpA gene of a bla(TEM)-carrying Tn3-like transposon, the latter itself being inserted by a transposition event. The IS26-composite transposon, which contains the blaOXA-1, aac6'-lb-cr and catB4 genes, was inserted into plasmids pEC_L8 and pEC_L46 by homologous recombination rather than a transposition event. Results obtained for pEC_L46 indicated that IS26 also plays an important role in structural rearrangements of the plasmid backbone and seems to facilitate the mobilisation of fragments from other plasmids. Conclusions: Collectively, these data suggests that IS26 together with ISEcp1 could play a critical role in the evolution of diverse multiresistant plasmids found in clinical Enterobacteriaceae.
Keywords
PLASMID, GENES, RESISTANCE, CTX-M, DNA-SEQUENCE, SPECTRUM-BETA-LACTAMASE, MESSENGER-RNA, ENTEROBACTERIACEAE, DISSEMINATION, SALMONELLA

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MLA
Smet, Annemieke et al. “Complete Nucleotide Sequence of CTX-M-15-plasmids from Clinical Escherichia Coli Isolates: Insertional Events of Transposons and Insertion Sequences.” PLOS ONE 5.6 (2010): n. pag. Print.
APA
Smet, Annemieke, Van Nieuwerburgh, F., Vandekerckhove, T., Martel, A., Deforce, D., Butaye, P., & Haesebrouck, F. (2010). Complete nucleotide sequence of CTX-M-15-plasmids from clinical Escherichia coli isolates: insertional events of transposons and insertion sequences. PLOS ONE, 5(6).
Chicago author-date
Smet, Annemieke, Filip Van Nieuwerburgh, Tom Vandekerckhove, An Martel, Dieter Deforce, Patrick Butaye, and Freddy Haesebrouck. 2010. “Complete Nucleotide Sequence of CTX-M-15-plasmids from Clinical Escherichia Coli Isolates: Insertional Events of Transposons and Insertion Sequences.” Plos One 5 (6).
Chicago author-date (all authors)
Smet, Annemieke, Filip Van Nieuwerburgh, Tom Vandekerckhove, An Martel, Dieter Deforce, Patrick Butaye, and Freddy Haesebrouck. 2010. “Complete Nucleotide Sequence of CTX-M-15-plasmids from Clinical Escherichia Coli Isolates: Insertional Events of Transposons and Insertion Sequences.” Plos One 5 (6).
Vancouver
1.
Smet A, Van Nieuwerburgh F, Vandekerckhove T, Martel A, Deforce D, Butaye P, et al. Complete nucleotide sequence of CTX-M-15-plasmids from clinical Escherichia coli isolates: insertional events of transposons and insertion sequences. PLOS ONE. 2010;5(6).
IEEE
[1]
A. Smet et al., “Complete nucleotide sequence of CTX-M-15-plasmids from clinical Escherichia coli isolates: insertional events of transposons and insertion sequences,” PLOS ONE, vol. 5, no. 6, 2010.
@article{1086016,
  abstract     = {Background: CTX-M-producing Escherichia coli strains are regarded as major global pathogens.
Methodology/Principal Findings: The nucleotide sequence of three plasmids (pEC_B24: 73801-bp; pEC_L8: 118525-bp and pEC_L46: 144871-bp) from Escherichia coli isolates obtained from patients with urinary tract infections and one plasmid (pEC_Bactec: 92970-bp) from an Escherichia coli strain isolated from the joint of a horse with arthritis were determined. Plasmid pEC_Bactec belongs to the Incl1 group and carries two resistance genes: bla(TEM-1) and bla(CTX-M-15). It shares more than 90% homology with a previously published bla(CTX-M)-plasmid from E. coli of human origin. Plasmid pEC_B24 belongs to the IncFII group whereas plasmids pEC_L8 and pEC_L46 represent a fusion of two replicons of type FII and FIA. On the pEC_B24 backbone, two resistance genes, blaTEM-1 and blaCTX-M-15, were found. Six resistance genes, blaTEM-1, blaCTX-M-15, bla(OXA-1), aac6'-lb-cr, tetA and catB4, were detected on the pEC_L8 backbone. The same antimicrobial drug resistance genes, with the exception of tetA, were also identified on the pEC_L46 backbone. Genome analysis of all 4 plasmids studied provides evidence of a seemingly frequent transposition event of the bla(CTX-M-15)-ISEcp1 element. This element seems to have a preferred insertion site at the tnpA gene of a bla(TEM)-carrying Tn3-like transposon, the latter itself being inserted by a transposition event. The IS26-composite transposon, which contains the blaOXA-1, aac6'-lb-cr and catB4 genes, was inserted into plasmids pEC_L8 and pEC_L46 by homologous recombination rather than a transposition event. Results obtained for pEC_L46 indicated that IS26 also plays an important role in structural rearrangements of the plasmid backbone and seems to facilitate the mobilisation of fragments from other plasmids.
Conclusions: Collectively, these data suggests that IS26 together with ISEcp1 could play a critical role in the evolution of diverse multiresistant plasmids found in clinical Enterobacteriaceae.},
  articleno    = {e11202},
  author       = {Smet, Annemieke and Van Nieuwerburgh, Filip and Vandekerckhove, Tom and Martel, An and Deforce, Dieter and Butaye, Patrick and Haesebrouck, Freddy},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  keywords     = {PLASMID,GENES,RESISTANCE,CTX-M,DNA-SEQUENCE,SPECTRUM-BETA-LACTAMASE,MESSENGER-RNA,ENTEROBACTERIACEAE,DISSEMINATION,SALMONELLA},
  language     = {eng},
  number       = {6},
  pages        = {8},
  title        = {Complete nucleotide sequence of CTX-M-15-plasmids from clinical Escherichia coli isolates: insertional events of transposons and insertion sequences},
  url          = {http://dx.doi.org/10.1371/journal.pone.0011202},
  volume       = {5},
  year         = {2010},
}

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