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The metabolisation of different iodinated peptide species

Bart De Spiegeleer (UGent) , Sylvia Van Dorpe (UGent) and Ewald Pauwels (UGent)
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HPC-UGent: the central High Performance Computing infrastructure of Ghent University
Abstract
Radioiodinated I-123, I-125 or I-131 labelled peptides are often used in biomedical research and diagnostic-therapeutic clinical practice. Moreover, iodine is a bio-essential element with specific physiology and biochemistry. Two enzymatic systems are known to handle iodinated organics: iodotyrosinedeiodinase (IYD) and iodothyroninedeiodinase (ID). However, at this moment, only iodinated amino acids and the thyroid hormones have been studied, but no systematic study on the metabolic fate of iodinated peptides is available. Using HPLC-DAD/MS/MS and HALO-columns, we have characterised different iodinated mouse obestatin peptides towards their in vitro metabolisation. The degradation kinetics and metabolites of the different iodinated obestatin-peptides arising from both enzymatic proteolysis and dehalogenation is studied.

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Citation

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Chicago
De Spiegeleer, Bart, Sylvia Van Dorpe, and Ewald Pauwels. 2010. “The Metabolisation of Different Iodinated Peptide Species.” In Peptides 2010 : Tales of Peptides : Proceedings of the Thirty-first European Peptide Symposium, ed. Michal Lebl, Morten Meldal, Knud J Jensen, and Thomas Høeg-Jensen, 502–503. European Peptide Society.
APA
De Spiegeleer, B., Van Dorpe, S., & Pauwels, E. (2010). The metabolisation of different iodinated peptide species. In M. Lebl, M. Meldal, K. J. Jensen, & T. Høeg-Jensen (Eds.), Peptides 2010 : tales of peptides : proceedings of the thirty-first European Peptide Symposium (pp. 502–503). Presented at the 31st European Peptide Symposium, European Peptide Society.
Vancouver
1.
De Spiegeleer B, Van Dorpe S, Pauwels E. The metabolisation of different iodinated peptide species. In: Lebl M, Meldal M, Jensen KJ, Høeg-Jensen T, editors. Peptides 2010 : tales of peptides : proceedings of the thirty-first European Peptide Symposium. European Peptide Society; 2010. p. 502–3.
MLA
De Spiegeleer, Bart, Sylvia Van Dorpe, and Ewald Pauwels. “The Metabolisation of Different Iodinated Peptide Species.” Peptides 2010 : Tales of Peptides : Proceedings of the Thirty-first European Peptide Symposium. Ed. Michal Lebl et al. European Peptide Society, 2010. 502–503. Print.
@inproceedings{1083649,
  abstract     = {Radioiodinated I-123, I-125 or I-131 labelled peptides are often used in biomedical research and diagnostic-therapeutic clinical practice. Moreover, iodine is a bio-essential element with specific physiology and biochemistry. Two enzymatic systems are known to handle iodinated organics: iodotyrosinedeiodinase (IYD) and iodothyroninedeiodinase (ID). However, at this moment, only iodinated amino acids and the thyroid hormones have been studied, but no systematic study on the metabolic fate of iodinated peptides is available.
Using HPLC-DAD/MS/MS and HALO-columns, we have characterised different iodinated mouse obestatin peptides towards their in vitro metabolisation. The degradation kinetics and metabolites of the different iodinated obestatin-peptides arising from both enzymatic proteolysis and dehalogenation is studied.},
  author       = {De Spiegeleer, Bart and Van Dorpe, Sylvia and Pauwels, Ewald},
  booktitle    = {Peptides 2010 : tales of peptides : proceedings of the thirty-first European Peptide Symposium},
  editor       = {Lebl, Michal and Meldal, Morten and Jensen, Knud J and H{\o}eg-Jensen, Thomas},
  isbn         = {0-9715560-5-9},
  language     = {eng},
  location     = {Copenhagen, Denmark},
  pages        = {502--503},
  publisher    = {European Peptide Society},
  title        = {The metabolisation of different iodinated peptide species},
  year         = {2010},
}