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Effect of induction of subacute ruminal acidosis on milk fat profile and rumen parameters

Ellen Colman UGent, WB Fokking, M Craninx, JR Newbold, Bernard De Baets UGent and Veerle Fievez UGent (2010) JOURNAL OF DAIRY SCIENCE. 93(10). p.4759-4773
abstract
High-concentrate diets can lead to subacute ruminal acidosis and are known to result in changes of the ruminal fermentation pattern and mammary secretion of fatty acids. The objective of this paper is to describe modifications in milk fatty acid proportions, particularly odd- and branched-chain fatty acids and rumen biohydrogenation intermediates, associated with rumen parameters during a 6-wk subacute ruminal acidosis induction protocol with 12 ruminally fistulated multiparous cows. The protocol involved a weekly gradual replacement of a standard dairy concentrate with a wheat-based concentrate (610 g of wheat/kg of concentrate) during the first 5 wk and an increase in the total amount of concentrate in wk 6. Before the end of induction wk 6, cows were switched to a control diet because 7 cows showed signs of sickness. The pH was measured continuously by an indwelling pH probe. Milk and rumen samples were taken on d 2 and 7 of each week. Data were analyzed using a linear mixed model and by principal component analysis. A pH decrease occurred after the first concentrate switch but rumen parameters returned to the original values and remained stable until wk 5. In wk 5 and 6, rumen pH values were indicative of increasing acidotic conditions. After switching to the control diet in wk 6; rumen pH values rapidly achieved normal values. Odd- and branched-chain fatty acids and C18:1 trans-10 increased with increasing amount of concentrate in the diet, whereas C18:1 trans-11 decreased. Four fatty acids [C18:1 trans-10, C15:0 and C17:0+C17:1 cis-9 (negative loadings), and iso C14:0 (positive loading)] largely correlated with the first principal component (PC1); with cows spread along the PC1 axis. The first 4 wk of the induction experiment showed variation across the second principal component (PC2) only, with high loadings of anteiso C13:0 (negative loading) and C18:2 cis-9,trans-11 and C18:1 trans-11 (positive loadings). Weeks 5 and 6 deviated from PC2 and tended toward the negative PC1 axis. A discriminant analysis using a stepwise approach indicated the main fatty acids discriminating between the control and acidotic samples as iso C13:0, iso C16:0, and C18:2 cis-9. trans-11 rather than milk fat content. or C18:1 trans-10; which have been used before as indicators of acidosis. This shows that specific milk fatty acids have potential in discriminating acidotic cases.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
CONJUGATED LINOLEIC-ACID, milk fat profile, rumen parameter, subacute ruminal acidosis, DAIRY-COWS, FERMENTATION PATTERN, ODD-CHAIN, STARCH, DIET, PH
journal title
JOURNAL OF DAIRY SCIENCE
J. Dairy Sci.
volume
93
issue
10
pages
4759 - 4773
Web of Science type
Article
Web of Science id
000282139900033
JCR category
AGRICULTURE, DAIRY & ANIMAL SCIENCE
JCR impact factor
2.497 (2010)
JCR rank
2/54 (2010)
JCR quartile
1 (2010)
ISSN
0022-0302
DOI
10.3168/jds.2010-3158
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1083273
handle
http://hdl.handle.net/1854/LU-1083273
date created
2010-12-06 11:02:42
date last changed
2011-10-31 00:31:01
@article{1083273,
  abstract     = {High-concentrate diets can lead to subacute ruminal acidosis and are known to result in changes of the ruminal fermentation pattern and mammary secretion of fatty acids. The objective of this paper is to describe modifications in milk fatty acid proportions, particularly odd- and branched-chain fatty acids and rumen biohydrogenation intermediates, associated with rumen parameters during a 6-wk subacute ruminal acidosis induction protocol with 12 ruminally fistulated multiparous cows. The protocol involved a weekly gradual replacement of a standard dairy concentrate with a wheat-based concentrate (610 g of wheat/kg of concentrate) during the first 5 wk and an increase in the total amount of concentrate in wk 6. Before the end of induction wk 6, cows were switched to a control diet because 7 cows showed signs of sickness. The pH was measured continuously by an indwelling pH probe. Milk and rumen samples were taken on d 2 and 7 of each week. Data were analyzed using a linear mixed model and by principal component analysis. A pH decrease occurred after the first concentrate switch but rumen parameters returned to the original values and remained stable until wk 5. In wk 5 and 6, rumen pH values were indicative of increasing acidotic conditions. After switching to the control diet in wk 6; rumen pH values rapidly achieved normal values. Odd- and branched-chain fatty acids and C18:1 trans-10 increased with increasing amount of concentrate in the diet, whereas C18:1 trans-11 decreased. Four fatty acids [C18:1 trans-10, C15:0 and C17:0+C17:1 cis-9 (negative loadings), and iso C14:0 (positive loading)] largely correlated with the first principal component (PC1); with cows spread along the PC1 axis. The first 4 wk of the induction experiment showed variation across the second principal component (PC2) only, with high loadings of anteiso C13:0 (negative loading) and C18:2 cis-9,trans-11 and C18:1 trans-11 (positive loadings). Weeks 5 and 6 deviated from PC2 and tended toward the negative PC1 axis. A discriminant analysis using a stepwise approach indicated the main fatty acids discriminating between the control and acidotic samples as iso C13:0, iso C16:0, and C18:2 cis-9. trans-11 rather than milk fat content. or C18:1 trans-10; which have been used before as indicators of acidosis. This shows that specific milk fatty acids have potential in discriminating acidotic cases.},
  author       = {Colman, Ellen and Fokking, WB and Craninx, M and Newbold, JR and De Baets, Bernard and Fievez, Veerle},
  issn         = {0022-0302},
  journal      = {JOURNAL OF DAIRY SCIENCE},
  keyword      = {CONJUGATED LINOLEIC-ACID,milk fat profile,rumen parameter,subacute ruminal acidosis,DAIRY-COWS,FERMENTATION PATTERN,ODD-CHAIN,STARCH,DIET,PH},
  language     = {eng},
  number       = {10},
  pages        = {4759--4773},
  title        = {Effect of induction of subacute ruminal acidosis on milk fat profile and rumen parameters},
  url          = {http://dx.doi.org/10.3168/jds.2010-3158},
  volume       = {93},
  year         = {2010},
}

Chicago
Colman, Ellen, WB Fokking, M Craninx, JR Newbold, Bernard De Baets, and Veerle Fievez. 2010. “Effect of Induction of Subacute Ruminal Acidosis on Milk Fat Profile and Rumen Parameters.” Journal of Dairy Science 93 (10): 4759–4773.
APA
Colman, Ellen, Fokking, W., Craninx, M., Newbold, J., De Baets, B., & Fievez, V. (2010). Effect of induction of subacute ruminal acidosis on milk fat profile and rumen parameters. JOURNAL OF DAIRY SCIENCE, 93(10), 4759–4773.
Vancouver
1.
Colman E, Fokking W, Craninx M, Newbold J, De Baets B, Fievez V. Effect of induction of subacute ruminal acidosis on milk fat profile and rumen parameters. JOURNAL OF DAIRY SCIENCE. 2010;93(10):4759–73.
MLA
Colman, Ellen, WB Fokking, M Craninx, et al. “Effect of Induction of Subacute Ruminal Acidosis on Milk Fat Profile and Rumen Parameters.” JOURNAL OF DAIRY SCIENCE 93.10 (2010): 4759–4773. Print.