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Multi locus sequence typing of Chlamydia reveals an association between Chlamydia psittaci genotypes and host species

Yvonne Pannekoek, Veerle Dickx UGent, Delphine Sylvie Anne Beeckman UGent, Keith A Jolley, Wendy C Keijzers, Evangelia Vretou, Martin CJ Maiden, Daisy Vanrompay UGent and Arie van der Ende (2010) PLOS ONE. 5(12).
abstract
Chlamydia comprises a group of obligate intracellular bacterial parasites responsible for a variety of diseases in humans and animals, including several zoonoses. Chlamydia trachomatis causes diseases such as trachoma, urogenital infection and lymphogranuloma venereum with severe morbidity. Chlamydia pneumoniae is a common cause of community-acquired respiratory tract infections. Chlamydia psittaci, causing zoonotic pneumonia in humans, is usually hosted by birds, while Chlamydia abortus, causing abortion and fetal death in mammals, including humans, is mainly hosted by goats and sheep. We used multi-locus sequence typing to asses the population structure of Chlamydia. In total, 132 Chlamydia isolates were analyzed, including 60 C. trachomatis, 18 C. pneumoniae, 16 C. abortus, 34 C. psittaci and one of each of C. pecorum, C. caviae, C. muridarum and C. felis. Cluster analyses utilizing the Neighbour-Joining algorithm with the maximum composite likelihood model of concatenated sequences of 7 housekeeping fragments showed that C. psittaci 84/2334 isolated from a parrot grouped together with the C. abortus isolates from goats and sheep. Cluster analyses of the individual alleles showed that in all instances C. psittaci 84/2334 formed one group with C. abortus. Moving 84/2334 from the C. psittaci group to the C. abortus group resulted in a significant increase in the number of fixed differences and elimination of the number of shared mutations between C. psittaci and C. abortus. C. psittaci M56 from a muskrat branched separately from the main group of C. psittaci isolates. C. psittaci genotypes appeared to be associated with host species. The phylogentic tree of C. psittaci did not follow that of its host bird species, suggesting host species jumps. In conclusion, we report for the first time an association between C. psittaci genotypes with host species.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
OUTER-MEMBRANE PROTEIN, CHLAMYDOPHILA-PSITTACI, MONOCLONAL-ANTIBODIES, STRAINS, ABORTUS, PET BIRDS, VNTR ANALYSIS, INFECTION, MICROIMMUNOFLUORESCENCE TEST, ORDER CHLAMYDIALES
journal title
PLOS ONE
PLoS One
volume
5
issue
12
article_number
e14179
pages
9 pages
Web of Science type
Article
Web of Science id
000284868000005
JCR category
BIOLOGY
JCR impact factor
4.411 (2010)
JCR rank
12/84 (2010)
JCR quartile
1 (2010)
ISSN
1932-6203
DOI
10.1371/journal.pone.0014179
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
1083090
handle
http://hdl.handle.net/1854/LU-1083090
date created
2010-12-06 09:40:29
date last changed
2011-07-12 14:57:37
@article{1083090,
  abstract     = {Chlamydia comprises a group of obligate intracellular bacterial parasites responsible for a variety of diseases in humans and animals, including several zoonoses. Chlamydia trachomatis causes diseases such as trachoma, urogenital infection and lymphogranuloma venereum with severe morbidity. Chlamydia pneumoniae is a common cause of community-acquired respiratory tract infections. Chlamydia psittaci, causing zoonotic pneumonia in humans, is usually hosted by birds, while Chlamydia abortus, causing abortion and fetal death in mammals, including humans, is mainly hosted by goats and sheep. We used multi-locus sequence typing to asses the population structure of Chlamydia. In total, 132 Chlamydia isolates were analyzed, including 60 C. trachomatis, 18 C. pneumoniae, 16 C. abortus, 34 C. psittaci and one of each of C. pecorum, C. caviae, C. muridarum and C. felis. Cluster analyses utilizing the Neighbour-Joining algorithm with the maximum composite likelihood model of concatenated sequences of 7 housekeeping fragments showed that C. psittaci 84/2334 isolated from a parrot grouped together with the C. abortus isolates from goats and sheep. Cluster analyses of the individual alleles showed that in all instances C. psittaci 84/2334 formed one group with C. abortus. Moving 84/2334 from the C. psittaci group to the C. abortus group resulted in a significant increase in the number of fixed differences and elimination of the number of shared mutations between C. psittaci and C. abortus. C. psittaci M56 from a muskrat branched separately from the main group of C. psittaci isolates. C. psittaci genotypes appeared to be associated with host species. The phylogentic tree of C. psittaci did not follow that of its host bird species, suggesting host species jumps. In conclusion, we report for the first time an association between C. psittaci genotypes with host species.},
  articleno    = {e14179},
  author       = {Pannekoek, Yvonne and Dickx, Veerle and Beeckman, Delphine Sylvie Anne and Jolley, Keith A and Keijzers, Wendy C and Vretou, Evangelia and Maiden, Martin CJ and Vanrompay, Daisy and van der Ende, Arie},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  keyword      = {OUTER-MEMBRANE PROTEIN,CHLAMYDOPHILA-PSITTACI,MONOCLONAL-ANTIBODIES,STRAINS,ABORTUS,PET BIRDS,VNTR ANALYSIS,INFECTION,MICROIMMUNOFLUORESCENCE TEST,ORDER CHLAMYDIALES},
  language     = {eng},
  number       = {12},
  pages        = {9},
  title        = {Multi locus sequence typing of Chlamydia reveals an association between Chlamydia psittaci genotypes and host species},
  url          = {http://dx.doi.org/10.1371/journal.pone.0014179},
  volume       = {5},
  year         = {2010},
}

Chicago
Pannekoek, Yvonne, Veerle Dickx, Delphine Sylvie Anne Beeckman, Keith A Jolley, Wendy C Keijzers, Evangelia Vretou, Martin CJ Maiden, Daisy Vanrompay, and Arie van der Ende. 2010. “Multi Locus Sequence Typing of Chlamydia Reveals an Association Between Chlamydia Psittaci Genotypes and Host Species.” Plos One 5 (12).
APA
Pannekoek, Y., Dickx, V., Beeckman, D. S. A., Jolley, K. A., Keijzers, W. C., Vretou, E., Maiden, M. C., et al. (2010). Multi locus sequence typing of Chlamydia reveals an association between Chlamydia psittaci genotypes and host species. PLOS ONE, 5(12).
Vancouver
1.
Pannekoek Y, Dickx V, Beeckman DSA, Jolley KA, Keijzers WC, Vretou E, et al. Multi locus sequence typing of Chlamydia reveals an association between Chlamydia psittaci genotypes and host species. PLOS ONE. 2010;5(12).
MLA
Pannekoek, Yvonne, Veerle Dickx, Delphine Sylvie Anne Beeckman, et al. “Multi Locus Sequence Typing of Chlamydia Reveals an Association Between Chlamydia Psittaci Genotypes and Host Species.” PLOS ONE 5.12 (2010): n. pag. Print.