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Influence of damaging and wilting red clover on lipid metabolism during ensiling and in vitro rumen incubation

Gijs Van Ranst UGent, Veerle Fievez UGent, M Vande walle, J Van Waes, J De Riek and Erik Van Bockstaele UGent (2010) ANIMAL. 4(9). p.1528-1540
abstract
This paper describes the relationship between protein-bound phenols in red clover, induced by different degrees of damaging before wilting and varying wilting duration, and in silo lipid metabolism. The ultimate effect of these changes on rumen biohydrogenation is the second focus of this paper For this experiment, red clover, damaged to different degrees (not damaged (ND), crushing or frozen/thawing (FT)) before wilting (4 or 24 h) was ensiled. Different degrees of damaging and wilting duration lead to differences in polyphenol oxidase (PPO) activity, measured as increase in protein-bound phenols. Treatment effects on fatty acid (FA) content and composition, lipid fractions (free FAs, membrane lipids (ML) and neutral fraction) and lipolysis were further studied in the silage. In FT, red clover lipolysis was markedly lower in the first days after ensiling, but this largely disappeared after 60 days of ensiling, regardless of wilting duration. This suggests an inhibition of plant lipases in FT silages. After 60 days of ensiling no differences in lipid fractions could be found between any of the treatments and differences in lipolysis were caused by reduced FA proportions in ML of wilted FT red clover Fresh, wilted (24 h) after damaging (ND or FT) and ensiled (4 or 60 days; wilted 24 h; ND or FT) red clover were also incubated in rumen fluid to study the biohydrogenation of C18:3n-3 and C18:2n-6 in vitro. Silages (both 60 days and to a lower degree 4 days) showed a lower biohydrogenation compared with fresh and wilted forages, regardless of damaging. This suggests that lipids in ensiled red clover were more protected, but this protection was not enhanced by a higher amount of protein-bound phenols in wilted FT compared with ND red clover The reduction of rumen microbial biohydrogenation with duration of red clover ensiling seems in contrast to what is expected, namely a higher biohydrogenation when a higher amount of FFA is present. This merits further investigation in relation to strategies to activate PPO toward the embedding of lipids in phenol protein complexes.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
POLYPHENOL OXIDASE, CONJUGATED LINOLEIC-ACID, lipolysis, polyphenol oxidase, PUFA, FATTY-ACID-COMPOSITION, biohydrogenation, fatty acid, DIACYLGLYCEROL ACYLTRANSFERASE, BIOHYDROGENATION, SILAGE, LIPOLYSIS, FORAGES, MILK, TRIACYLGLYCEROL
journal title
ANIMAL
Animal
volume
4
issue
9
pages
1528 - 1540
Web of Science type
Article
Web of Science id
000281263500010
JCR category
VETERINARY SCIENCES
JCR impact factor
1.458 (2010)
JCR rank
32/145 (2010)
JCR quartile
1 (2010)
ISSN
1751-7311
DOI
10.1017/S1751731110000625
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1083059
handle
http://hdl.handle.net/1854/LU-1083059
date created
2010-12-06 09:15:04
date last changed
2011-10-01 00:31:01
@article{1083059,
  abstract     = {This paper describes the relationship between protein-bound phenols in red clover, induced by different degrees of damaging before wilting and varying wilting duration, and in silo lipid metabolism. The ultimate effect of these changes on rumen biohydrogenation is the second focus of this paper For this experiment, red clover, damaged to different degrees (not damaged (ND), crushing or frozen/thawing (FT)) before wilting (4 or 24 h) was ensiled. Different degrees of damaging and wilting duration lead to differences in polyphenol oxidase (PPO) activity, measured as increase in protein-bound phenols. Treatment effects on fatty acid (FA) content and composition, lipid fractions (free FAs, membrane lipids (ML) and neutral fraction) and lipolysis were further studied in the silage. In FT, red clover lipolysis was markedly lower in the first days after ensiling, but this largely disappeared after 60 days of ensiling, regardless of wilting duration. This suggests an inhibition of plant lipases in FT silages. After 60 days of ensiling no differences in lipid fractions could be found between any of the treatments and differences in lipolysis were caused by reduced FA proportions in ML of wilted FT red clover Fresh, wilted (24 h) after damaging (ND or FT) and ensiled (4 or 60 days; wilted 24 h; ND or FT) red clover were also incubated in rumen fluid to study the biohydrogenation of C18:3n-3 and C18:2n-6 in vitro. Silages (both 60 days and to a lower degree 4 days) showed a lower biohydrogenation compared with fresh and wilted forages, regardless of damaging. This suggests that lipids in ensiled red clover were more protected, but this protection was not enhanced by a higher amount of protein-bound phenols in wilted FT compared with ND red clover The reduction of rumen microbial biohydrogenation with duration of red clover ensiling seems in contrast to what is expected, namely a higher biohydrogenation when a higher amount of FFA is present. This merits further investigation in relation to strategies to activate PPO toward the embedding of lipids in phenol protein complexes.},
  author       = {Van Ranst, Gijs and Fievez, Veerle and Vande walle, M and Van Waes, J and De Riek, J and Van Bockstaele, Erik},
  issn         = {1751-7311},
  journal      = {ANIMAL},
  keyword      = {POLYPHENOL OXIDASE,CONJUGATED LINOLEIC-ACID,lipolysis,polyphenol oxidase,PUFA,FATTY-ACID-COMPOSITION,biohydrogenation,fatty acid,DIACYLGLYCEROL ACYLTRANSFERASE,BIOHYDROGENATION,SILAGE,LIPOLYSIS,FORAGES,MILK,TRIACYLGLYCEROL},
  language     = {eng},
  number       = {9},
  pages        = {1528--1540},
  title        = {Influence of damaging and wilting red clover on lipid metabolism during ensiling and in vitro rumen incubation},
  url          = {http://dx.doi.org/10.1017/S1751731110000625},
  volume       = {4},
  year         = {2010},
}

Chicago
Van Ranst, Gijs, Veerle Fievez, M Vande walle, J Van Waes, J De Riek, and Erik Van Bockstaele. 2010. “Influence of Damaging and Wilting Red Clover on Lipid Metabolism During Ensiling and in Vitro Rumen Incubation.” Animal 4 (9): 1528–1540.
APA
Van Ranst, G., Fievez, V., Vande walle, M., Van Waes, J., De Riek, J., & Van Bockstaele, E. (2010). Influence of damaging and wilting red clover on lipid metabolism during ensiling and in vitro rumen incubation. ANIMAL, 4(9), 1528–1540.
Vancouver
1.
Van Ranst G, Fievez V, Vande walle M, Van Waes J, De Riek J, Van Bockstaele E. Influence of damaging and wilting red clover on lipid metabolism during ensiling and in vitro rumen incubation. ANIMAL. 2010;4(9):1528–40.
MLA
Van Ranst, Gijs, Veerle Fievez, M Vande walle, et al. “Influence of Damaging and Wilting Red Clover on Lipid Metabolism During Ensiling and in Vitro Rumen Incubation.” ANIMAL 4.9 (2010): 1528–1540. Print.