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Insights into the venom composition of the ectoparasitoid wasp Nasonia vitripennis from bioinformatic and proteomic studies

Dirk de Graaf UGent, Maarten Aerts UGent, Marleen Brunain UGent, CA Desjardins, Franciscus Jacobs UGent, JH Werren and Bart Devreese UGent (2010) INSECT MOLECULAR BIOLOGY. 19(suppl. 1). p.11-26
abstract
With the Nasonia vitripennis genome sequences available, we attempted to determine the proteins present in venom by two different approaches. First, we searched for the transcripts of venom proteins by a bioinformatic approach using amino acid sequences of known hymenopteran venom proteins. Second, we performed proteomic analyses of crude N. vitripennis venom removed from the venom reservoir, implementing both an off-line two-dimensional liquid chromatography matrix-assisted laser desorption/ionization time-of-flight (2D-LC-MALDI-TOF) mass spectrometry (MS) and a two-dimensional liquid chromatography electrospray ionization Founer transform ion cyclotron resonance (2D-LC-ESI-FT-ICR) MS setup. This combination of bioinformatic and proteomic studies resulted in an extraordinary richness of identified venom constituents. Moreover, half of the 79 identified proteins were not yet associated with insect venoms: 16 proteins showed similarity only to known proteins from other tissues or secretions, and an additional 23 did not show similarity to any known protein. Serine proteases and their inhibitors were the most represented. Fifteen nonsecretory proteins were also identified by proteomic means and probably represent so-called 'venom trace elements'. The present study contributes greatly to the understanding of the biological diversity of the venom of parasitoid wasps at the molecular level.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
venom, proteomics, Nasonia vitripennis, Hymenoptera, genome mining, KAZAL-TYPE INHIBITOR, BULLATA-PARKER DIPTERA, PIMPLA-HYPOCHONDRIACA, WALKER HYMENOPTERA, SARCOPHAGA-BULLATA, MOLECULAR-CLONING, HONEY-BEE, FLESH FLY, EVOLUTIONARY FAMILIES, ENDOPARASITOID VENOM
journal title
INSECT MOLECULAR BIOLOGY
Insect Mol. Biol.
volume
19
issue
suppl. 1
pages
11 - 26
Web of Science type
Article
Web of Science id
000273947400004
JCR category
ENTOMOLOGY
JCR impact factor
2.669 (2010)
JCR rank
5/83 (2010)
JCR quartile
1 (2010)
ISSN
0962-1075
DOI
10.1111/j.1365-2583.2009.00914.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1080951
handle
http://hdl.handle.net/1854/LU-1080951
date created
2010-11-30 13:45:43
date last changed
2010-12-20 16:06:35
@article{1080951,
  abstract     = {With the Nasonia vitripennis genome sequences available, we attempted to determine the proteins present in venom by two different approaches. First, we searched for the transcripts of venom proteins by a bioinformatic approach using amino acid sequences of known hymenopteran venom proteins. Second, we performed proteomic analyses of crude N. vitripennis venom removed from the venom reservoir, implementing both an off-line two-dimensional liquid chromatography matrix-assisted laser desorption/ionization time-of-flight (2D-LC-MALDI-TOF) mass spectrometry (MS) and a two-dimensional liquid chromatography electrospray ionization Founer transform ion cyclotron resonance (2D-LC-ESI-FT-ICR) MS setup. This combination of bioinformatic and proteomic studies resulted in an extraordinary richness of identified venom constituents. Moreover, half of the 79 identified proteins were not yet associated with insect venoms: 16 proteins showed similarity only to known proteins from other tissues or secretions, and an additional 23 did not show similarity to any known protein. Serine proteases and their inhibitors were the most represented. Fifteen nonsecretory proteins were also identified by proteomic means and probably represent so-called 'venom trace elements'. The present study contributes greatly to the understanding of the biological diversity of the venom of parasitoid wasps at the molecular level.},
  author       = {de Graaf, Dirk and Aerts, Maarten and Brunain, Marleen and Desjardins, CA and Jacobs, Franciscus and Werren, JH and Devreese, Bart},
  issn         = {0962-1075},
  journal      = {INSECT MOLECULAR BIOLOGY},
  keyword      = {venom,proteomics,Nasonia vitripennis,Hymenoptera,genome mining,KAZAL-TYPE INHIBITOR,BULLATA-PARKER DIPTERA,PIMPLA-HYPOCHONDRIACA,WALKER HYMENOPTERA,SARCOPHAGA-BULLATA,MOLECULAR-CLONING,HONEY-BEE,FLESH FLY,EVOLUTIONARY FAMILIES,ENDOPARASITOID VENOM},
  language     = {eng},
  number       = {suppl. 1},
  pages        = {11--26},
  title        = {Insights into the venom composition of the ectoparasitoid wasp Nasonia vitripennis from bioinformatic and proteomic studies},
  url          = {http://dx.doi.org/10.1111/j.1365-2583.2009.00914.x},
  volume       = {19},
  year         = {2010},
}

Chicago
de Graaf, Dirk, Maarten Aerts, Marleen Brunain, CA Desjardins, Franciscus Jacobs, JH Werren, and Bart Devreese. 2010. “Insights into the Venom Composition of the Ectoparasitoid Wasp Nasonia Vitripennis from Bioinformatic and Proteomic Studies.” Insect Molecular Biology 19 (suppl. 1): 11–26.
APA
de Graaf, Dirk, Aerts, M., Brunain, M., Desjardins, C., Jacobs, F., Werren, J., & Devreese, B. (2010). Insights into the venom composition of the ectoparasitoid wasp Nasonia vitripennis from bioinformatic and proteomic studies. INSECT MOLECULAR BIOLOGY, 19(suppl. 1), 11–26.
Vancouver
1.
de Graaf D, Aerts M, Brunain M, Desjardins C, Jacobs F, Werren J, et al. Insights into the venom composition of the ectoparasitoid wasp Nasonia vitripennis from bioinformatic and proteomic studies. INSECT MOLECULAR BIOLOGY. 2010;19(suppl. 1):11–26.
MLA
de Graaf, Dirk, Maarten Aerts, Marleen Brunain, et al. “Insights into the Venom Composition of the Ectoparasitoid Wasp Nasonia Vitripennis from Bioinformatic and Proteomic Studies.” INSECT MOLECULAR BIOLOGY 19.suppl. 1 (2010): 11–26. Print.