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Longitudinal quantification of inflammation in the murine DSS-induced colitis model using μPET/CT

Pieter Hindryckx UGent, Steven Staelens UGent, Lindsey Devisscher UGent, Steven Deleye UGent, Filip De Vos UGent, Louke Delrue UGent, Harald Peeters UGent, Debby Laukens UGent and MARTINE DE VOS UGent (2011) INFLAMMATORY BOWEL DISEASES. 17(10). p.2058-2064
abstract
Background: This study investigates whether deoxy-2-[18F]fluoro-d-glucose (FDG) micro-positron emission tomography (mu PET)/computed tomography (CT) can serve as a tool for monitoring of the commonly used dextran sodium sulfate (DSS)-induced murine model of inflammatory bowel disease (IBD). Methods: DSS-colitis was induced in Sv129 mice. In a first experiment, four animals were serially scanned with CT and FDG-mu PET on days 0, 3, 7, 11, and 14. The ratio of the mean voxel count of the PET images in the colon and the brain was compared with the histological inflammation score and the colonic myeloperoxidase levels. A second experiment was performed to investigate whether FDG-mu PET was able to detect differences in inflammation between two DSS-treated groups, one receiving placebo (n = 4) and one receiving dimethyloxalylglycine (DMOG) (n = 4), a compound that protects against DSS-induced colitis. Results: The progression of the colonic/brain FDG-signal ratio (over days 0-14) agreed with the predicted histological inflammation score, obtained from a parallel DSS-experiment. Moreover, the quantification of normalized colonic FDG-activity at the final timepoint (day 14) showed an excellent correlation with both the MPO levels (Spearman's rho 1) and the histological inflammation score (Spearman's rho = 0.949) of the scanned mice. The protective action of DMOG in DSS colitis was clearly demonstrated with FDG-mu PET/CT (normalized colonic FDG-activity DMOG versus placebo: P < 0.05). Conclusions: FDG-mu PET-CT is a feasible and reliable noninvasive method to monitor murine DSS-induced colitis. The implementation of this technique in this widely used IBD model opens a new window for pathophysiological research and high-throughput screening of potential therapeutic compounds in preclinical IBD research.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
experimental colitis, BOWEL-DISEASE, mu PET/CT, small animal molecular imaging, MICE, IN-SITU
journal title
INFLAMMATORY BOWEL DISEASES
Inflamm. Bowel Dis.
volume
17
issue
10
pages
2058 - 2064
Web of Science type
Article
Web of Science id
000295140800010
JCR category
GASTROENTEROLOGY & HEPATOLOGY
JCR impact factor
4.855 (2011)
JCR rank
12/73 (2011)
JCR quartile
1 (2011)
ISSN
1078-0998
DOI
10.1002/ibd.21578
project
Ghent researchers on unfolded proteins in inflammatory disease (GROUP-ID)
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1078720
handle
http://hdl.handle.net/1854/LU-1078720
date created
2010-11-23 17:36:48
date last changed
2013-02-27 09:08:14
@article{1078720,
  abstract     = {Background: This study investigates whether deoxy-2-[18F]fluoro-d-glucose (FDG) micro-positron emission tomography (mu PET)/computed tomography (CT) can serve as a tool for monitoring of the commonly used dextran sodium sulfate (DSS)-induced murine model of inflammatory bowel disease (IBD). Methods: DSS-colitis was induced in Sv129 mice. In a first experiment, four animals were serially scanned with CT and FDG-mu PET on days 0, 3, 7, 11, and 14. The ratio of the mean voxel count of the PET images in the colon and the brain was compared with the histological inflammation score and the colonic myeloperoxidase levels. A second experiment was performed to investigate whether FDG-mu PET was able to detect differences in inflammation between two DSS-treated groups, one receiving placebo (n = 4) and one receiving dimethyloxalylglycine (DMOG) (n = 4), a compound that protects against DSS-induced colitis. Results: The progression of the colonic/brain FDG-signal ratio (over days 0-14) agreed with the predicted histological inflammation score, obtained from a parallel DSS-experiment. Moreover, the quantification of normalized colonic FDG-activity at the final timepoint (day 14) showed an excellent correlation with both the MPO levels (Spearman's rho 1) and the histological inflammation score (Spearman's rho = 0.949) of the scanned mice. The protective action of DMOG in DSS colitis was clearly demonstrated with FDG-mu PET/CT (normalized colonic FDG-activity DMOG versus placebo: P {\textlangle} 0.05). Conclusions: FDG-mu PET-CT is a feasible and reliable noninvasive method to monitor murine DSS-induced colitis. The implementation of this technique in this widely used IBD model opens a new window for pathophysiological research and high-throughput screening of potential therapeutic compounds in preclinical IBD research.},
  author       = {Hindryckx, Pieter and Staelens, Steven and Devisscher, Lindsey and Deleye, Steven and De Vos, Filip and Delrue, Louke and Peeters, Harald and Laukens, Debby and DE VOS, MARTINE},
  issn         = {1078-0998},
  journal      = {INFLAMMATORY BOWEL DISEASES},
  keyword      = {experimental colitis,BOWEL-DISEASE,mu PET/CT,small animal molecular imaging,MICE,IN-SITU},
  language     = {eng},
  number       = {10},
  pages        = {2058--2064},
  title        = {Longitudinal quantification of inflammation in the murine DSS-induced colitis model using \ensuremath{\mu}PET/CT},
  url          = {http://dx.doi.org/10.1002/ibd.21578},
  volume       = {17},
  year         = {2011},
}

Chicago
HINDRYCKX, PIETER, Steven Staelens, Lindsey Devisscher, Steven Deleye, Filip De Vos, Louke Delrue, Harald Peeters, Debby Laukens, and MARTINE DE VOS. 2011. “Longitudinal Quantification of Inflammation in the Murine DSS-induced Colitis Model Using μPET/CT.” Inflammatory Bowel Diseases 17 (10): 2058–2064.
APA
HINDRYCKX, P., Staelens, S., Devisscher, L., Deleye, S., De Vos, F., Delrue, L., Peeters, H., et al. (2011). Longitudinal quantification of inflammation in the murine DSS-induced colitis model using μPET/CT. INFLAMMATORY BOWEL DISEASES, 17(10), 2058–2064.
Vancouver
1.
HINDRYCKX P, Staelens S, Devisscher L, Deleye S, De Vos F, Delrue L, et al. Longitudinal quantification of inflammation in the murine DSS-induced colitis model using μPET/CT. INFLAMMATORY BOWEL DISEASES. 2011;17(10):2058–64.
MLA
HINDRYCKX, PIETER, Steven Staelens, Lindsey Devisscher, et al. “Longitudinal Quantification of Inflammation in the Murine DSS-induced Colitis Model Using μPET/CT.” INFLAMMATORY BOWEL DISEASES 17.10 (2011): 2058–2064. Print.