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P-cadherin counteracts myosin II-B function: implications in melanoma progression

Koen Jacobs UGent, Mireille Van Gele UGent, Ramses Forsyth UGent, Lieve Brochez UGent, Barbara Vanhoecke UGent, Olivier De Wever UGent and Marc Bracke UGent (2010) MOLECULAR CANCER. 9.
abstract
BACKGROUND: Malignant transformation of melanocytes is frequently attended by a switch in cadherin expression profile as shown for E- and N-cadherin. For P-cadherin, downregulation in metastasizing melanoma has been demonstrated, and over-expression of P-cadherin in melanoma cell lines has been shown to inhibit invasion. The strong invasive and metastatic nature of cutaneous melanoma implies a deregulated interplay between intercellular adhesion and migration-related molecules RESULTS: In this study we performed a microarray analysis to compare the mRNA expression profile of an invasive BLM melanoma cell line (BLM LIE) and the non-invasive P-cadherin over-expression variant (BLM P-cad). Results indicate that nonmuscle myosin II-B is downregulated in BLM P-cad. Moreover, myosin II-B plays a major role in melanoma migration and invasiveness by retracting the tail during the migratory cycle, as shown by the localization of myosin II-B stress fibers relative to Golgi and the higher levels of phosphorylated myosin light chain. Analysis of P-cadherin and myosin II-B in nodular melanoma sections and in a panel of melanoma cell lines further confirmed that there is an inverse relationship between both molecules. CONCLUSIONS: Therefore, we conclude that P-cadherin counteracts the expression and function of myosin II-B, resulting in the suppression of the invasive and migratory behaviour of BLM melanoma cells.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
RHO-FAMILY GTPASES, CULTURED-CELLS, BREAST-CANCER, EXPRESSION, INVASION, MIGRATION, PHOSPHORYLATION, LOCALIZATION, ACTIVATION, MORPHOLOGY
journal title
MOLECULAR CANCER
Mol. Cancer
volume
9
article_number
255
pages
12 pages
Web of Science type
Article
Web of Science id
000282464700001
JCR category
ONCOLOGY
JCR impact factor
3.779 (2010)
JCR rank
54/181 (2010)
JCR quartile
2 (2010)
ISSN
1476-4598
DOI
10.1186/1476-4598-9-255
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
1073043
handle
http://hdl.handle.net/1854/LU-1073043
date created
2010-11-10 14:42:56
date last changed
2010-11-10 16:58:30
@article{1073043,
  abstract     = {BACKGROUND: Malignant transformation of melanocytes is frequently attended by a switch in cadherin expression profile as shown for E- and N-cadherin. For P-cadherin, downregulation in metastasizing melanoma has been demonstrated, and over-expression of P-cadherin in melanoma cell lines has been shown to inhibit invasion. The strong invasive and metastatic nature of cutaneous melanoma implies a deregulated interplay between intercellular adhesion and migration-related molecules
RESULTS: In this study we performed a microarray analysis to compare the mRNA expression profile of an invasive BLM melanoma cell line (BLM LIE) and the non-invasive P-cadherin over-expression variant (BLM P-cad). Results indicate that nonmuscle myosin II-B is downregulated in BLM P-cad. Moreover, myosin II-B plays a major role in melanoma migration and invasiveness by retracting the tail during the migratory cycle, as shown by the localization of myosin II-B stress fibers relative to Golgi and the higher levels of phosphorylated myosin light chain. Analysis of P-cadherin and myosin II-B in nodular melanoma sections and in a panel of melanoma cell lines further confirmed that there is an inverse relationship between both molecules.
CONCLUSIONS: Therefore, we conclude that P-cadherin counteracts the expression and function of myosin II-B, resulting in the suppression of the invasive and migratory behaviour of BLM melanoma cells.},
  articleno    = {255},
  author       = {Jacobs, Koen and Van Gele, Mireille and Forsyth, Ramses and Brochez, Lieve and Vanhoecke, Barbara and De Wever, Olivier and Bracke, Marc},
  issn         = {1476-4598},
  journal      = {MOLECULAR CANCER},
  keyword      = {RHO-FAMILY GTPASES,CULTURED-CELLS,BREAST-CANCER,EXPRESSION,INVASION,MIGRATION,PHOSPHORYLATION,LOCALIZATION,ACTIVATION,MORPHOLOGY},
  language     = {eng},
  pages        = {12},
  title        = {P-cadherin counteracts myosin II-B function: implications in melanoma progression},
  url          = {http://dx.doi.org/10.1186/1476-4598-9-255},
  volume       = {9},
  year         = {2010},
}

Chicago
Jacobs, Koen, Mireille Van Gele, Ramses Forsyth, Lieve Brochez, Barbara Vanhoecke, Olivier De Wever, and Marc Bracke. 2010. “P-cadherin Counteracts Myosin II-B Function: Implications in Melanoma Progression.” Molecular Cancer 9.
APA
Jacobs, Koen, Van Gele, M., Forsyth, R., Brochez, L., Vanhoecke, B., De Wever, O., & Bracke, M. (2010). P-cadherin counteracts myosin II-B function: implications in melanoma progression. MOLECULAR CANCER, 9.
Vancouver
1.
Jacobs K, Van Gele M, Forsyth R, Brochez L, Vanhoecke B, De Wever O, et al. P-cadherin counteracts myosin II-B function: implications in melanoma progression. MOLECULAR CANCER. 2010;9.
MLA
Jacobs, Koen, Mireille Van Gele, Ramses Forsyth, et al. “P-cadherin Counteracts Myosin II-B Function: Implications in Melanoma Progression.” MOLECULAR CANCER 9 (2010): n. pag. Print.