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P-cadherin counteracts myosin II-B function: implications in melanoma progression

Koen Jacobs (UGent) , Mireille Van Gele (UGent) , Ramses Forsyth (UGent) , Lieve Brochez (UGent) , Barbara Vanhoecke (UGent) , Olivier De Wever (UGent) and Marc Bracke (UGent)
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Abstract
BACKGROUND: Malignant transformation of melanocytes is frequently attended by a switch in cadherin expression profile as shown for E- and N-cadherin. For P-cadherin, downregulation in metastasizing melanoma has been demonstrated, and over-expression of P-cadherin in melanoma cell lines has been shown to inhibit invasion. The strong invasive and metastatic nature of cutaneous melanoma implies a deregulated interplay between intercellular adhesion and migration-related molecules RESULTS: In this study we performed a microarray analysis to compare the mRNA expression profile of an invasive BLM melanoma cell line (BLM LIE) and the non-invasive P-cadherin over-expression variant (BLM P-cad). Results indicate that nonmuscle myosin II-B is downregulated in BLM P-cad. Moreover, myosin II-B plays a major role in melanoma migration and invasiveness by retracting the tail during the migratory cycle, as shown by the localization of myosin II-B stress fibers relative to Golgi and the higher levels of phosphorylated myosin light chain. Analysis of P-cadherin and myosin II-B in nodular melanoma sections and in a panel of melanoma cell lines further confirmed that there is an inverse relationship between both molecules. CONCLUSIONS: Therefore, we conclude that P-cadherin counteracts the expression and function of myosin II-B, resulting in the suppression of the invasive and migratory behaviour of BLM melanoma cells.
Keywords
RHO-FAMILY GTPASES, CULTURED-CELLS, BREAST-CANCER, EXPRESSION, INVASION, MIGRATION, PHOSPHORYLATION, LOCALIZATION, ACTIVATION, MORPHOLOGY

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Chicago
Jacobs, Koen, Mireille Van Gele, Ramses Forsyth, Lieve Brochez, Barbara Vanhoecke, Olivier De Wever, and Marc Bracke. 2010. “P-cadherin Counteracts Myosin II-B Function: Implications in Melanoma Progression.” Molecular Cancer 9.
APA
Jacobs, Koen, Van Gele, M., Forsyth, R., Brochez, L., Vanhoecke, B., De Wever, O., & Bracke, M. (2010). P-cadherin counteracts myosin II-B function: implications in melanoma progression. MOLECULAR CANCER, 9.
Vancouver
1.
Jacobs K, Van Gele M, Forsyth R, Brochez L, Vanhoecke B, De Wever O, et al. P-cadherin counteracts myosin II-B function: implications in melanoma progression. MOLECULAR CANCER. 2010;9.
MLA
Jacobs, Koen, Mireille Van Gele, Ramses Forsyth, et al. “P-cadherin Counteracts Myosin II-B Function: Implications in Melanoma Progression.” MOLECULAR CANCER 9 (2010): n. pag. Print.
@article{1073043,
  abstract     = {BACKGROUND: Malignant transformation of melanocytes is frequently attended by a switch in cadherin expression profile as shown for E- and N-cadherin. For P-cadherin, downregulation in metastasizing melanoma has been demonstrated, and over-expression of P-cadherin in melanoma cell lines has been shown to inhibit invasion. The strong invasive and metastatic nature of cutaneous melanoma implies a deregulated interplay between intercellular adhesion and migration-related molecules
RESULTS: In this study we performed a microarray analysis to compare the mRNA expression profile of an invasive BLM melanoma cell line (BLM LIE) and the non-invasive P-cadherin over-expression variant (BLM P-cad). Results indicate that nonmuscle myosin II-B is downregulated in BLM P-cad. Moreover, myosin II-B plays a major role in melanoma migration and invasiveness by retracting the tail during the migratory cycle, as shown by the localization of myosin II-B stress fibers relative to Golgi and the higher levels of phosphorylated myosin light chain. Analysis of P-cadherin and myosin II-B in nodular melanoma sections and in a panel of melanoma cell lines further confirmed that there is an inverse relationship between both molecules.
CONCLUSIONS: Therefore, we conclude that P-cadherin counteracts the expression and function of myosin II-B, resulting in the suppression of the invasive and migratory behaviour of BLM melanoma cells.},
  articleno    = {255},
  author       = {Jacobs, Koen and Van Gele, Mireille and Forsyth, Ramses and Brochez, Lieve and Vanhoecke, Barbara and De Wever, Olivier and Bracke, Marc},
  issn         = {1476-4598},
  journal      = {MOLECULAR CANCER},
  keyword      = {RHO-FAMILY GTPASES,CULTURED-CELLS,BREAST-CANCER,EXPRESSION,INVASION,MIGRATION,PHOSPHORYLATION,LOCALIZATION,ACTIVATION,MORPHOLOGY},
  language     = {eng},
  pages        = {12},
  title        = {P-cadherin counteracts myosin II-B function: implications in melanoma progression},
  url          = {http://dx.doi.org/10.1186/1476-4598-9-255},
  volume       = {9},
  year         = {2010},
}

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