Advanced search

Histology and ultrastructure of the midgut of germ-free Artemia franciscana nauplii

Author
Organization
Abstract
The morphology of the midgut of <i>Artemia</i> nauplii was studied using histology and transmission electron microscopy (TEM). Germ-free <i>Artemia</i> nauplii were fed with Baker’s yeast and dead <i>Aeromonas hydrophila</i>. For histology, two live nauplii were fixed at 2, 4 and 6 days of age in 100% ethyl alcohol, 40% formaldehyde and acetic acid for 5 min, dehydrated in graded alcohols and embedded in paraffin. Five µm thick sections were stained with haematoxylin and eosin and subsequently examined by light microscopy. For TEM, nauplii were fixed overnight in Karnovsky’s fixative, post-fixed overnight in 1% osmium tetroxyde and embedded in SPURR’s resin. Blocks were sectioned at 60 nm, sections were mounted on formvar coated grids and post-stained with uranyl acetate and lead citrate. They were examined using a Jeol 1200 EXII TEM at 80kV accelerating voltage. The entire midgut epithelium was composed of columnar to cuboidal cells. A clear brush border was visible in the apical part of the midgut cells. Large, central nuclei were characteristic. The cytoplasm contained numerous mitochondria and an abundant rough endoplasmic reticulum. Golgi complexes were seen in some cells. A basement membrane separates the epithelium from the underlying circular muscle layer. Future studies investigating the influence of microorganisms on germ-free <i>Artemia</i> will exploit the knowledge gathered in the present morphological study. It will allow the identification of potential morphological differences occurring in the midgut of <i>Artemia</i> nauplii when microorganisms are present in the culture compared to germ-free animals.
Keywords
transmission electron microscopy, Light microscopy

Citation

Please use this url to cite or link to this publication:

Chicago
Gunasekara, Rangalle, Anamaria Rekecki, Christophe Casteleyn, Maria Cornelissen, Peter Bossier, and Wim Van Den Broeck. 2010. “Histology and Ultrastructure of the Midgut of Germ-free Artemia Franciscana Nauplii.” In Benelux Congress of Zoology, 17th, Programme and Abstracts, 129–129. Royal Belgian Zoological Society (RBZS) ; Royal Dutch Zoological Society (RDZS).
APA
Gunasekara, R., Rekecki, A., Casteleyn, C., Cornelissen, M., Bossier, P., & Van Den Broeck, W. (2010). Histology and ultrastructure of the midgut of germ-free Artemia franciscana nauplii. Benelux Congress of Zoology, 17th, Programme and abstracts (pp. 129–129). Presented at the 17th Benelux Congress of Zoology : Classic biology in modern times, Royal Belgian Zoological Society (RBZS) ; Royal Dutch Zoological Society (RDZS).
Vancouver
1.
Gunasekara R, Rekecki A, Casteleyn C, Cornelissen M, Bossier P, Van Den Broeck W. Histology and ultrastructure of the midgut of germ-free Artemia franciscana nauplii. Benelux Congress of Zoology, 17th, Programme and abstracts. Royal Belgian Zoological Society (RBZS) ; Royal Dutch Zoological Society (RDZS); 2010. p. 129–129.
MLA
Gunasekara, Rangalle, Anamaria Rekecki, Christophe Casteleyn, et al. “Histology and Ultrastructure of the Midgut of Germ-free Artemia Franciscana Nauplii.” Benelux Congress of Zoology, 17th, Programme and Abstracts. Royal Belgian Zoological Society (RBZS) ; Royal Dutch Zoological Society (RDZS), 2010. 129–129. Print.
@inproceedings{1070974,
  abstract     = {The morphology of the midgut of {\textlangle}i{\textrangle}Artemia{\textlangle}/i{\textrangle} nauplii was studied using histology and transmission electron microscopy (TEM). Germ-free {\textlangle}i{\textrangle}Artemia{\textlangle}/i{\textrangle} nauplii were fed with Baker{\textquoteright}s yeast and dead {\textlangle}i{\textrangle}Aeromonas hydrophila{\textlangle}/i{\textrangle}. For histology, two live nauplii were fixed at 2, 4 and 6 days of age in 100\% ethyl alcohol, 40\% formaldehyde and acetic acid for 5 min, dehydrated in graded alcohols and embedded in paraffin. Five {\textmu}m thick sections were stained with haematoxylin and eosin and subsequently examined by light microscopy. For TEM, nauplii were fixed overnight in Karnovsky{\textquoteright}s fixative, post-fixed overnight in 1\% osmium tetroxyde and embedded in SPURR{\textquoteright}s resin. Blocks were sectioned at 60 nm, sections were mounted on formvar coated grids and post-stained with uranyl acetate and lead citrate. They were examined using a Jeol 1200 EXII TEM at 80kV accelerating voltage. The entire midgut epithelium was composed of columnar to cuboidal cells. A clear brush border was visible in the apical part of the midgut cells. Large, central nuclei were characteristic. The cytoplasm contained numerous mitochondria and an abundant rough endoplasmic reticulum. Golgi complexes were seen in some cells. A basement membrane separates the epithelium from the underlying circular muscle layer. Future studies investigating the influence of microorganisms on germ-free {\textlangle}i{\textrangle}Artemia{\textlangle}/i{\textrangle} will exploit the knowledge gathered in the present morphological study. It will allow the identification of potential morphological differences occurring in the midgut of {\textlangle}i{\textrangle}Artemia{\textlangle}/i{\textrangle} nauplii when microorganisms are present in the culture compared to germ-free animals.},
  author       = {Gunasekara, Rangalle and Rekecki, Anamaria and Casteleyn, Christophe and Cornelissen, Maria and Bossier, Peter and Van Den Broeck, Wim},
  booktitle    = {Benelux Congress of Zoology, 17th, Programme and abstracts},
  keyword      = {transmission electron microscopy,Light microscopy},
  language     = {eng},
  location     = {Ghent, Belgium},
  pages        = {129--129},
  publisher    = {Royal Belgian Zoological Society (RBZS) ; Royal Dutch Zoological Society (RDZS)},
  title        = {Histology and ultrastructure of the midgut of germ-free Artemia franciscana nauplii},
  url          = {http://www.bcz2010.ugent.be/sites/default/files/Programme\%20and\%20Abstracts.pdf},
  year         = {2010},
}