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Comparative gut morphology of mnn9 yeast and wild type yeast fed Artemia franciscana nauplii

Rangalle Gunasekara UGent, Anamaria Rekecki UGent, Christophe Casteleyn UGent, Maria Cornelissen UGent, Peter Bossier UGent and Wim Van Den Broeck UGent (2010) Benelux Congress of Zoology, 17th, Programme and abstracts. p.66-66
abstract
Germ-free <i>Artemia </i> nauplii were fed daily with Baker’s yeast (wild type strain) and dead <i>Aeromonas hydrophila </i> and named group WTY. The other group was fed daily with yeast mutant strain, mann9, and dead <i>Aeromonas hydrophila </i> (group mnY). For histology, two live nauplii per group were fixed at days 2, 4 and 6 in 100% ethyl alcohol, 40% formaldehyde and acetic acid for 5 min, dehydrated in graded alcohols and embedded in paraffin. Five µm thick sections were stained with haematoxylin and eosin and subsequently examined by light microscopy. For TEM, nauplii were fixed overnight in Karnovsky’s fixative, post-fixed overnight in 1% osmium tetroxyde and embedded in SPURR’s resin. Blocks were sectioned at 60 nm and sections were mounted on formvar coated grids and post-stained with uranyl acetate and lead citrate. They were examined using a Jeol 1200 EXII TEM at 80kV accelerating voltage. On day 2 no significant morphological differences in the mid- and hindgut could be observed between both groups. However, on days 4 and 6, significantly longer mid- and hindguts with larger diameters were seen in nauplii of the MnY group. Mnn9 yeast mutant strain sustains better growth under the described culture conditions. This could be due to high amounts of cell-wall chitin and glucans in combination with low amounts of mannose present in mnn9 yeast strain. Since <i>Artemia </i> exhibits low mannase activity in the digestive tract, diets with less mannose content make digestion easy. Furthermore, high levels of chitin and glucan make the diet more nutritious.
Please use this url to cite or link to this publication:
author
organization
year
type
conference
publication status
published
subject
keyword
yeast, histology, transmission electron microscopy
in
Benelux Congress of Zoology, 17th, Programme and abstracts
pages
66 - 66
publisher
Royal Belgian Zoological Society (RBZS) ; Royal Dutch Zoological Society (RDZS)
conference name
17th Benelux Congress of Zoology : Classic biology in modern times
conference location
Ghent, Belgium
conference start
2010-10-22
conference end
2010-10-23
language
English
UGent publication?
yes
classification
C3
id
1070745
handle
http://hdl.handle.net/1854/LU-1070745
alternative location
http://www.bcz2010.ugent.be/sites/default/files/Programme%20and%20Abstracts.pdf
date created
2010-11-05 11:33:28
date last changed
2010-11-05 13:36:40
@inproceedings{1070745,
  abstract     = {Germ-free {\textlangle}i{\textrangle}Artemia {\textlangle}/i{\textrangle}  nauplii were fed daily with Baker{\textquoteright}s yeast (wild type strain) and dead {\textlangle}i{\textrangle}Aeromonas hydrophila {\textlangle}/i{\textrangle} and named group WTY. The other group was fed daily with yeast mutant strain, mann9, and dead {\textlangle}i{\textrangle}Aeromonas hydrophila {\textlangle}/i{\textrangle} (group mnY). For histology, two live nauplii per group were fixed at days 2, 4 and 6 in 100\% ethyl alcohol, 40\% formaldehyde and acetic acid for 5 min, dehydrated in graded alcohols and embedded in paraffin. Five {\textmu}m thick sections were stained with haematoxylin and eosin and subsequently examined by light microscopy. For TEM, nauplii were fixed overnight in Karnovsky{\textquoteright}s fixative, post-fixed overnight in 1\% osmium tetroxyde and embedded in SPURR{\textquoteright}s resin. Blocks were sectioned at 60 nm and sections were mounted on formvar coated grids and post-stained with uranyl acetate and lead citrate. They were examined using a Jeol 1200 EXII TEM at 80kV accelerating voltage. On day 2 no significant morphological differences in the mid- and hindgut could be observed between both groups. However, on days 4 and 6, significantly longer mid- and hindguts with larger diameters were seen in nauplii of the MnY group.  Mnn9 yeast mutant strain sustains better growth under the described culture conditions. This could be due to high amounts of cell-wall chitin and glucans in combination with low amounts of mannose present in mnn9 yeast strain. Since {\textlangle}i{\textrangle}Artemia {\textlangle}/i{\textrangle} exhibits low mannase activity in the digestive tract, diets with less mannose content make digestion easy. Furthermore, high levels of chitin and glucan make the diet more nutritious.},
  author       = {Gunasekara, Rangalle and Rekecki, Anamaria and Casteleyn, Christophe and Cornelissen, Maria and Bossier, Peter and Van Den Broeck, Wim},
  booktitle    = {Benelux Congress of Zoology, 17th, Programme and abstracts},
  keyword      = {yeast,histology,transmission electron microscopy},
  language     = {eng},
  location     = {Ghent, Belgium},
  pages        = {66--66},
  publisher    = {Royal Belgian Zoological Society (RBZS) ; Royal Dutch Zoological Society (RDZS)},
  title        = {Comparative gut morphology of mnn9 yeast and wild type yeast fed Artemia franciscana nauplii},
  url          = {http://www.bcz2010.ugent.be/sites/default/files/Programme\%20and\%20Abstracts.pdf},
  year         = {2010},
}

Chicago
Gunasekara, Rangalle, Anamaria Rekecki, Christophe Casteleyn, Maria Cornelissen, Peter Bossier, and Wim Van Den Broeck. 2010. “Comparative Gut Morphology of Mnn9 Yeast and Wild Type Yeast Fed Artemia Franciscana Nauplii.” In Benelux Congress of Zoology, 17th, Programme and Abstracts, 66–66. Royal Belgian Zoological Society (RBZS) ; Royal Dutch Zoological Society (RDZS).
APA
Gunasekara, R., Rekecki, A., Casteleyn, C., Cornelissen, M., Bossier, P., & Van Den Broeck, W. (2010). Comparative gut morphology of mnn9 yeast and wild type yeast fed Artemia franciscana nauplii. Benelux Congress of Zoology, 17th, Programme and abstracts (pp. 66–66). Presented at the 17th Benelux Congress of Zoology : Classic biology in modern times, Royal Belgian Zoological Society (RBZS) ; Royal Dutch Zoological Society (RDZS).
Vancouver
1.
Gunasekara R, Rekecki A, Casteleyn C, Cornelissen M, Bossier P, Van Den Broeck W. Comparative gut morphology of mnn9 yeast and wild type yeast fed Artemia franciscana nauplii. Benelux Congress of Zoology, 17th, Programme and abstracts. Royal Belgian Zoological Society (RBZS) ; Royal Dutch Zoological Society (RDZS); 2010. p. 66–66.
MLA
Gunasekara, Rangalle, Anamaria Rekecki, Christophe Casteleyn, et al. “Comparative Gut Morphology of Mnn9 Yeast and Wild Type Yeast Fed Artemia Franciscana Nauplii.” Benelux Congress of Zoology, 17th, Programme and Abstracts. Royal Belgian Zoological Society (RBZS) ; Royal Dutch Zoological Society (RDZS), 2010. 66–66. Print.