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Stereology and computer assisted three-dimensional reconstruction as tools to study probiotic effects of Aeromonas hydrophila on the digestive tract of germ-free Artemia franciscana nauplii

Rangalle Gunasekara UGent, Pieter Cornillie UGent, Christophe Casteleyn UGent, Ward De Spiegelaere UGent, Patrick Sorgeloos UGent, Paul Simoens UGent, Peter Bossier UGent and Wim Van Den Broeck UGent (2011) JOURNAL OF APPLIED MICROBIOLOGY. 110(1). p.98-105
abstract
Aims: Validation of stereology and three-dimensional reconstruction for monitoring the probiotic effect of Aeromonas hydrophila on the gut development of germ-free Artemia franciscana nauplii. Methods and Results: Germ-free Artemia nauplii were cultured using Baker’s yeast and dead Aer. hydrophila. Live Aer. hydrophila were added on the first day to the treatment group. The gut length and volume were monitored on days two and four using stereology and three-dimensional reconstruction. Both methods showed comparable results. Stereology was least labour intensive to estimate volumes, while three-dimensional reconstructions rendered architectural and topographical data of the gut. Moreover, a positive effect of probiotic bacterium, Aer. hydrophila is likely. Conclusion: Slight increment in the growth of the digestive tract of A. franciscana nauplii exerted by probiotic bacteria could be detected using stereology and three-dimensional reconstruction. Significance and Impact of the Study: The gnotobiotic Artemia rearing system is unique to investigate the effects of micro-organisms on the development of nauplii. However, in the base of this model system, only survival counts and length measurements exist as monitoring tools. Therefore, additional tools such as stereology and three-dimensional reconstruction are prerequisite to obtain more powerful analysis.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
probiotic bacteria, three-dimensional reconstruction, digestive tract, germ-free, SPP., PERFORMANCE, YEAST, BACTERIAL STRAINS, CELL-WALL MUTANTS, GNOTOBIOTICALLY-GROWN ARTEMIA, stereology
journal title
JOURNAL OF APPLIED MICROBIOLOGY
J. Appl. Microbiol.
volume
110
issue
1
pages
98 - 105
Web of Science type
Article
Web of Science id
000285207600011
JCR category
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
JCR impact factor
2.337 (2011)
JCR rank
71/157 (2011)
JCR quartile
2 (2011)
ISSN
1364-5072
DOI
10.1111/j.1365-2672.2010.04862.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1070718
handle
http://hdl.handle.net/1854/LU-1070718
date created
2010-11-05 11:12:23
date last changed
2011-07-06 16:48:16
@article{1070718,
  abstract     = {Aims: Validation of stereology and three-dimensional reconstruction for monitoring the probiotic effect of Aeromonas hydrophila on the gut development of germ-free Artemia franciscana nauplii.
Methods and Results: Germ-free Artemia nauplii were cultured using Baker{\textquoteright}s yeast and dead Aer. hydrophila. Live Aer. hydrophila were added on the first day to the treatment group. The gut length and volume were monitored on days two and four using stereology and three-dimensional reconstruction. Both methods showed comparable results. Stereology was least labour intensive to estimate volumes, while three-dimensional reconstructions rendered architectural and topographical data of the gut. Moreover, a positive effect of probiotic bacterium, Aer. hydrophila is likely.
Conclusion: Slight increment in the growth of the digestive tract of A. franciscana nauplii exerted by probiotic bacteria could be detected using stereology and three-dimensional reconstruction.
Significance and Impact of the Study: The gnotobiotic Artemia rearing system is unique to investigate the effects of micro-organisms on the development of nauplii. However, in the base of this model system, only survival counts and length measurements exist as monitoring tools. Therefore, additional tools such as stereology and three-dimensional reconstruction are prerequisite to obtain more powerful analysis.},
  author       = {Gunasekara, Rangalle and Cornillie, Pieter and Casteleyn, Christophe and De Spiegelaere, Ward and Sorgeloos, Patrick and Simoens, Paul and Bossier, Peter and Van Den Broeck, Wim},
  issn         = {1364-5072},
  journal      = {JOURNAL OF APPLIED MICROBIOLOGY},
  keyword      = {probiotic bacteria,three-dimensional reconstruction,digestive tract,germ-free,SPP.,PERFORMANCE,YEAST,BACTERIAL STRAINS,CELL-WALL MUTANTS,GNOTOBIOTICALLY-GROWN ARTEMIA,stereology},
  language     = {eng},
  number       = {1},
  pages        = {98--105},
  title        = {Stereology and computer assisted three-dimensional reconstruction as tools to study probiotic effects of Aeromonas hydrophila on the digestive tract of germ-free Artemia franciscana nauplii},
  url          = {http://dx.doi.org/10.1111/j.1365-2672.2010.04862.x},
  volume       = {110},
  year         = {2011},
}

Chicago
Gunasekara, Rangalle, Pieter Cornillie, Christophe Casteleyn, WARD DE SPIEGELAERE, Patrick Sorgeloos, Paul Simoens, Peter Bossier, and Wim Van Den Broeck. 2011. “Stereology and Computer Assisted Three-dimensional Reconstruction as Tools to Study Probiotic Effects of Aeromonas Hydrophila on the Digestive Tract of Germ-free Artemia Franciscana Nauplii.” Journal of Applied Microbiology 110 (1): 98–105.
APA
Gunasekara, R., Cornillie, P., Casteleyn, C., DE SPIEGELAERE, W., Sorgeloos, P., Simoens, P., Bossier, P., et al. (2011). Stereology and computer assisted three-dimensional reconstruction as tools to study probiotic effects of Aeromonas hydrophila on the digestive tract of germ-free Artemia franciscana nauplii. JOURNAL OF APPLIED MICROBIOLOGY, 110(1), 98–105.
Vancouver
1.
Gunasekara R, Cornillie P, Casteleyn C, DE SPIEGELAERE W, Sorgeloos P, Simoens P, et al. Stereology and computer assisted three-dimensional reconstruction as tools to study probiotic effects of Aeromonas hydrophila on the digestive tract of germ-free Artemia franciscana nauplii. JOURNAL OF APPLIED MICROBIOLOGY. 2011;110(1):98–105.
MLA
Gunasekara, Rangalle, Pieter Cornillie, Christophe Casteleyn, et al. “Stereology and Computer Assisted Three-dimensional Reconstruction as Tools to Study Probiotic Effects of Aeromonas Hydrophila on the Digestive Tract of Germ-free Artemia Franciscana Nauplii.” JOURNAL OF APPLIED MICROBIOLOGY 110.1 (2011): 98–105. Print.