Histogenic analysis of chemically induced mixoploids in Spathiphyllum wallisii
- Author
- Ives Vanstechelman (UGent) , Tom Eeckhaut, Johan Van Huylenbroeck and Marie-Christine Van Labeke (UGent)
- Organization
- Abstract
- Tetraploids and mixoploids were induced in several Spathiphyllum wallisii genotypes through in vitro application of mitosis inhibitors. Flow cytometry of leaves enabled the identification of sectorial hybrids, whereas microscopic nuclei research combined with root flow cytometry was required to provide insights in the histogenic composition of the mixoploids and to identify periclinal chimeras. Microscopic observation of epidermal or parenchymatic cell areas or the average cell thickness did not allow unequivocal characterization. However, direct visualization of diploid and tetraploid cells was enabled by analysis of nuclear areas. Root analysis was performed by classical fluorescence of metaphase chromosome spreads. This study showed the necessity of microscopic techniques for a thorough characterization of mixoploids. It also demonstrated the potential of the combination of these techniques with flow cytometry to unravel the exact effect of mitosis inhibitors on monocot plants. Finally, it might help to optimize the tetraploidization efficiency.
- Keywords
- ANGIOSPERMS, COLCHICINE, TETRAPLOIDS, ARABIDOPSIS, INDUCTION, INVITRO, ROSA, POLYPLOIDIZATION, Ploidy chimeras, Periclinal, In vitro, Histogenic layers, Fluorescence, Sectorial, CYTOMETRIC ANALYSIS, CELL-SIZE
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-1070404
- MLA
- Vanstechelman, Ives, et al. “Histogenic Analysis of Chemically Induced Mixoploids in Spathiphyllum Wallisii.” EUPHYTICA, vol. 174, no. 1, 2010, pp. 61–72, doi:10.1007/s10681-010-0133-z.
- APA
- Vanstechelman, I., Eeckhaut, T., Van Huylenbroeck, J., & Van Labeke, M.-C. (2010). Histogenic analysis of chemically induced mixoploids in Spathiphyllum wallisii. EUPHYTICA, 174(1), 61–72. https://doi.org/10.1007/s10681-010-0133-z
- Chicago author-date
- Vanstechelman, Ives, Tom Eeckhaut, Johan Van Huylenbroeck, and Marie-Christine Van Labeke. 2010. “Histogenic Analysis of Chemically Induced Mixoploids in Spathiphyllum Wallisii.” EUPHYTICA 174 (1): 61–72. https://doi.org/10.1007/s10681-010-0133-z.
- Chicago author-date (all authors)
- Vanstechelman, Ives, Tom Eeckhaut, Johan Van Huylenbroeck, and Marie-Christine Van Labeke. 2010. “Histogenic Analysis of Chemically Induced Mixoploids in Spathiphyllum Wallisii.” EUPHYTICA 174 (1): 61–72. doi:10.1007/s10681-010-0133-z.
- Vancouver
- 1.Vanstechelman I, Eeckhaut T, Van Huylenbroeck J, Van Labeke M-C. Histogenic analysis of chemically induced mixoploids in Spathiphyllum wallisii. EUPHYTICA. 2010;174(1):61–72.
- IEEE
- [1]I. Vanstechelman, T. Eeckhaut, J. Van Huylenbroeck, and M.-C. Van Labeke, “Histogenic analysis of chemically induced mixoploids in Spathiphyllum wallisii,” EUPHYTICA, vol. 174, no. 1, pp. 61–72, 2010.
@article{1070404,
abstract = {{Tetraploids and mixoploids were induced in several Spathiphyllum wallisii genotypes through in vitro application of mitosis inhibitors. Flow cytometry of leaves enabled the identification of sectorial hybrids, whereas microscopic nuclei research combined with root flow cytometry was required to provide insights in the histogenic composition of the mixoploids and to identify periclinal chimeras. Microscopic observation of epidermal or parenchymatic cell areas or the average cell thickness did not allow unequivocal characterization. However, direct visualization of diploid and tetraploid cells was enabled by analysis of nuclear areas. Root analysis was performed by classical fluorescence of metaphase chromosome spreads. This study showed the necessity of microscopic techniques for a thorough characterization of mixoploids. It also demonstrated the potential of the combination of these techniques with flow cytometry to unravel the exact effect of mitosis inhibitors on monocot plants. Finally, it might help to optimize the tetraploidization efficiency.}},
author = {{Vanstechelman, Ives and Eeckhaut, Tom and Van Huylenbroeck, Johan and Van Labeke, Marie-Christine}},
issn = {{0014-2336}},
journal = {{EUPHYTICA}},
keywords = {{ANGIOSPERMS,COLCHICINE,TETRAPLOIDS,ARABIDOPSIS,INDUCTION,INVITRO,ROSA,POLYPLOIDIZATION,Ploidy chimeras,Periclinal,In vitro,Histogenic layers,Fluorescence,Sectorial,CYTOMETRIC ANALYSIS,CELL-SIZE}},
language = {{eng}},
number = {{1}},
pages = {{61--72}},
title = {{Histogenic analysis of chemically induced mixoploids in Spathiphyllum wallisii}},
url = {{http://dx.doi.org/10.1007/s10681-010-0133-z}},
volume = {{174}},
year = {{2010}},
}
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