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Dinucleoside polyphosphates: newly detected uraemic compounds with an impact on leucocyte oxidative burst

Eva Schepers UGent, Griet Glorieux UGent, Vera Jankowski, Annemieke Dhondt UGent, Joachim Jankowski and Raymond Vanholder UGent (2010) NEPHROLOGY DIALYSIS TRANSPLANTATION. 25(8). p.2636-2644
abstract
Background. Dinucleoside polyphosphates (NpnN) have pathophysiologic roles in cardiovascular disease and are newly detected uraemic retention solutes. They were retrieved in human plasma, tissues and cells. Although their impact on several cell systems involved in vascular damage (endothelium, smooth muscle cells and thrombocytes) has been evaluated, their effect on different types of leucocytes has never been studied. Methods. This study evaluates, for the first time, the impact of NpnN on monocyte, granulocyte and lymphocyte oxidative burst activity at baseline and after stimulation with N-formyl-methionine-leucine-phenylalanine (fMLP) and phorbol 12-myristate 13-acetate (PMA) in whole blood. Diadenosine triphosphate (Ap(3)A) to diadenosine hexaphosphate (Ap(6)A) were tested to investigate the effect of the number of phosphate groups on reactive oxygen species (ROS) production. The effect of the type of nucleoside was evaluated by comparing adenosine guanosine tetraphosphate, diguanosine tetraphosphate, uridine adenosine tetraphosphate (Up(4)A) and diadenosine tetraphosphate (Ap(4)A). Results. This study demonstrated that lymphocytes are especially susceptible to intracellular diadenosine polyphosphates. Depending on the phosphate chain length, different effects were observed. At baseline and with fMLP Ap(4)A, Ap(5)A and Ap(6)A enhanced lymphocyted-free radical production. In addition, Ap(3)A, Ap(4)A and Ap5A increased PMA-stimulated ROS production in lymphocytes. Monocytes and granulocytes parallel the lymphocyte response albeit with an inhibition of Ap(6)A on granulocytes. Considering NpnN with four phosphate groups, Up(4)A showed the most important stimulatory effects on monocytes and Ap(4)A on lymphocytes. Conclusions. NpnN mainly have a leucocyte-activating impact, most significant for Ap(4)A, considering phosphate chain length, and for Up(4)A, considering the type of nucleosides. These results suggest that the pro-inflammatory effects of NpnN can contribute to the development of atherosclerosis, probably in the early stages of chronic kidney disease, but their chemical composition affects their activity.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
P2 RECEPTORS, NEUTROPHIL APOPTOSIS, ADENINE-DINUCLEOTIDES, HEMODIALYSIS-PATIENTS, CARDIOVASCULAR-DISEASE, COLONY-STIMULATING FACTOR, URIDINE ADENOSINE-TETRAPHOSPHATE, DIADENOSINE POLYPHOSPHATES, GLOMERULAR-FILTRATION-RATE, CHRONIC KIDNEY-DISEASE, oxidative stress, uraemic toxins, cell activation, cardiovascular disease, chronic kidney disease
journal title
NEPHROLOGY DIALYSIS TRANSPLANTATION
Nephrol. Dial. Transplant.
volume
25
issue
8
pages
2636 - 2644
Web of Science type
Article
Web of Science id
000281483300040
JCR category
UROLOGY & NEPHROLOGY
JCR impact factor
3.564 (2010)
JCR rank
12/68 (2010)
JCR quartile
1 (2010)
ISSN
0931-0509
DOI
10.1093/ndt/gfq080
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1068648
handle
http://hdl.handle.net/1854/LU-1068648
date created
2010-11-03 08:49:03
date last changed
2011-09-01 00:30:54
@article{1068648,
  abstract     = {Background. Dinucleoside polyphosphates (NpnN) have pathophysiologic roles in cardiovascular disease and are newly detected uraemic retention solutes. They were retrieved in human plasma, tissues and cells. Although their impact on several cell systems involved in vascular damage (endothelium, smooth muscle cells and thrombocytes) has been evaluated, their effect on different types of leucocytes has never been studied.
Methods. This study evaluates, for the first time, the impact of NpnN on monocyte, granulocyte and lymphocyte oxidative burst activity at baseline and after stimulation with N-formyl-methionine-leucine-phenylalanine (fMLP) and phorbol 12-myristate 13-acetate (PMA) in whole blood. Diadenosine triphosphate (Ap(3)A) to diadenosine hexaphosphate (Ap(6)A) were tested to investigate the effect of the number of phosphate groups on reactive oxygen species (ROS) production. The effect of the type of nucleoside was evaluated by comparing adenosine guanosine tetraphosphate, diguanosine tetraphosphate, uridine adenosine tetraphosphate (Up(4)A) and diadenosine tetraphosphate (Ap(4)A).
Results. This study demonstrated that lymphocytes are especially susceptible to intracellular diadenosine polyphosphates. Depending on the phosphate chain length, different effects were observed. At baseline and with fMLP Ap(4)A, Ap(5)A and Ap(6)A enhanced lymphocyted-free radical production. In addition, Ap(3)A, Ap(4)A and Ap5A increased PMA-stimulated ROS production in lymphocytes. Monocytes and granulocytes parallel the lymphocyte response albeit with an inhibition of Ap(6)A on granulocytes. Considering NpnN with four phosphate groups, Up(4)A showed the most important stimulatory effects on monocytes and Ap(4)A on lymphocytes.
Conclusions. NpnN mainly have a leucocyte-activating impact, most significant for Ap(4)A, considering phosphate chain length, and for Up(4)A, considering the type of nucleosides. These results suggest that the pro-inflammatory effects of NpnN can contribute to the development of atherosclerosis, probably in the early stages of chronic kidney disease, but their chemical composition affects their activity.},
  author       = {Schepers, Eva and Glorieux, Griet and Jankowski, Vera and Dhondt, Annemieke and Jankowski, Joachim and Vanholder, Raymond},
  issn         = {0931-0509},
  journal      = {NEPHROLOGY DIALYSIS TRANSPLANTATION},
  keyword      = {P2 RECEPTORS,NEUTROPHIL APOPTOSIS,ADENINE-DINUCLEOTIDES,HEMODIALYSIS-PATIENTS,CARDIOVASCULAR-DISEASE,COLONY-STIMULATING FACTOR,URIDINE ADENOSINE-TETRAPHOSPHATE,DIADENOSINE POLYPHOSPHATES,GLOMERULAR-FILTRATION-RATE,CHRONIC KIDNEY-DISEASE,oxidative stress,uraemic toxins,cell activation,cardiovascular disease,chronic kidney disease},
  language     = {eng},
  number       = {8},
  pages        = {2636--2644},
  title        = {Dinucleoside polyphosphates: newly detected uraemic compounds with an impact on leucocyte oxidative burst},
  url          = {http://dx.doi.org/10.1093/ndt/gfq080},
  volume       = {25},
  year         = {2010},
}

Chicago
Schepers, Eva, Griet Glorieux, Vera Jankowski, Annemieke Dhondt, Joachim Jankowski, and Raymond Vanholder. 2010. “Dinucleoside Polyphosphates: Newly Detected Uraemic Compounds with an Impact on Leucocyte Oxidative Burst.” Nephrology Dialysis Transplantation 25 (8): 2636–2644.
APA
Schepers, E., Glorieux, G., Jankowski, V., Dhondt, A., Jankowski, J., & Vanholder, R. (2010). Dinucleoside polyphosphates: newly detected uraemic compounds with an impact on leucocyte oxidative burst. NEPHROLOGY DIALYSIS TRANSPLANTATION, 25(8), 2636–2644.
Vancouver
1.
Schepers E, Glorieux G, Jankowski V, Dhondt A, Jankowski J, Vanholder R. Dinucleoside polyphosphates: newly detected uraemic compounds with an impact on leucocyte oxidative burst. NEPHROLOGY DIALYSIS TRANSPLANTATION. 2010;25(8):2636–44.
MLA
Schepers, Eva, Griet Glorieux, Vera Jankowski, et al. “Dinucleoside Polyphosphates: Newly Detected Uraemic Compounds with an Impact on Leucocyte Oxidative Burst.” NEPHROLOGY DIALYSIS TRANSPLANTATION 25.8 (2010): 2636–2644. Print.