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The substrate specificity profile of human granzyme A

(2010) BIOLOGICAL CHEMISTRY. 391(8). p.983-997
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Abstract
The exact biological function of granzyme A, a granule-associated serine protease belonging to the tryptase family of proteases, is still a matter of debate because conflicting roles have been suggested, such as initiation of caspase-independent apoptosis-like cell death and endogenous modulation of inflammatory processes. In contrast to its well-studied family member, granzyme B, far less is known about the physiological targets of granzyme A. Using an N-terminal peptide-centric proteomics technology, the substrate specificity of human granzyme A was extensively characterized at the level of macromolecular protein substrates. Overall, more than 260 cleavage sites, almost exclusively favoring basic residues at the P1 position, in approximately 200 unique protein substrates, including the well-known in vitro substrates APEX-endonuclease 1 and different histones, were identified. Further substrate characterization was used to delineate physical properties in the substrate specificity profiles, which further highlights important aspects in protease/substrate biology.
Keywords
proteolytic hotspots, protease degradomics, granzyme A, COFRADIC, proteomics, LYMPHOCYTE PROTEASE GRANZYME, INDEPENDENT CELL-DEATH, N-TERMINAL PEPTIDES, AMINO-ACID-RESIDUES, SECONDARY STRUCTURE, MEDIATED APOPTOSIS, ACTIVATED DNASE, CLEAVES, PREDICTION, PROTEINS

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Chicago
Van Damme, Petra, Sebastian Maurer-Stroh, Han Hao, Niklaas Colaert, Evy Timmerman, Frank Eisenhaber, Joël Vandekerckhove, and Kris Gevaert. 2010. “The Substrate Specificity Profile of Human Granzyme A.” Biological Chemistry 391 (8): 983–997.
APA
Van Damme, Petra, Maurer-Stroh, S., Hao, H., Colaert, N., Timmerman, E., Eisenhaber, F., Vandekerckhove, J., et al. (2010). The substrate specificity profile of human granzyme A. BIOLOGICAL CHEMISTRY, 391(8), 983–997. Presented at the 6th General Meeting of the International-Proteolysis-Society.
Vancouver
1.
Van Damme P, Maurer-Stroh S, Hao H, Colaert N, Timmerman E, Eisenhaber F, et al. The substrate specificity profile of human granzyme A. BIOLOGICAL CHEMISTRY. 2010;391(8):983–97.
MLA
Van Damme, Petra, Sebastian Maurer-Stroh, Han Hao, et al. “The Substrate Specificity Profile of Human Granzyme A.” BIOLOGICAL CHEMISTRY 391.8 (2010): 983–997. Print.
@article{1064461,
  abstract     = {The exact biological function of granzyme A, a granule-associated serine protease belonging to the tryptase family of proteases, is still a matter of debate because conflicting roles have been suggested, such as initiation of caspase-independent apoptosis-like cell death and endogenous modulation of inflammatory processes. In contrast to its well-studied family member, granzyme B, far less is known about the physiological targets of granzyme A. Using an N-terminal peptide-centric proteomics technology, the substrate specificity of human granzyme A was extensively characterized at the level of macromolecular protein substrates. Overall, more than 260 cleavage sites, almost exclusively favoring basic residues at the P1 position, in approximately 200 unique protein substrates, including the well-known in vitro substrates APEX-endonuclease 1 and different histones, were identified. Further substrate characterization was used to delineate physical properties in the substrate specificity profiles, which further highlights important aspects in protease/substrate biology.},
  author       = {Van Damme, Petra and Maurer-Stroh, Sebastian and Hao, Han and Colaert, Niklaas and Timmerman, Evy and Eisenhaber, Frank and Vandekerckhove, Jo{\"e}l and Gevaert, Kris},
  issn         = {1431-6730},
  journal      = {BIOLOGICAL CHEMISTRY},
  keyword      = {proteolytic hotspots,protease degradomics,granzyme A,COFRADIC,proteomics,LYMPHOCYTE PROTEASE GRANZYME,INDEPENDENT CELL-DEATH,N-TERMINAL PEPTIDES,AMINO-ACID-RESIDUES,SECONDARY STRUCTURE,MEDIATED APOPTOSIS,ACTIVATED DNASE,CLEAVES,PREDICTION,PROTEINS},
  language     = {eng},
  location     = {Gold Coast, Australia},
  number       = {8},
  pages        = {983--997},
  title        = {The substrate specificity profile of human granzyme A},
  url          = {http://dx.doi.org/10.1515/BC.2010.096},
  volume       = {391},
  year         = {2010},
}

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