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Influence of 5-HT4 receptor activation on acetylcholine release in human large intestine with endometriosis

Romain Lefebvre UGent, S Ferrero, Inge Van Colen UGent, Ingeborg Dhaese UGent, G Camerini, E Fulcheri and V Remorgida (2010) NEUROGASTROENTEROLOGY AND MOTILITY. 22(5). p.557-563
abstract
Background: The 5-HT4 receptor agonist prucalopride enhances large intestinal contractility by facilitating acetylcholine release through activation of 5-HT4 receptors on cholinergic nerves and is effective in patients with constipation. Patients with intestinal endometriosis can present with constipation. We investigated in vitro whether large intestinal endometriotic infiltration influences contractility and facilitation of acetylcholine release by prucalopride. Methods: Sigmoid colon or rectum circular muscle strips were obtained at the level of an endometriotic nodule with infiltration of the Auerbach plexus, and at a macroscopically healthy site at least 5 cm cranially from the nodule, in patients undergoing laparoscopic colorectal resection because of symptomatic bowel endometriosis. Responses to muscarinic receptor stimulation and to electrical field stimulation (EFS), and the facilitating effect of prucalopride on acetylcholine release were evaluated. Key results: The EC50 and E-max of the contractile responses to the muscarinic receptor agonist carbachol did not differ between healthy and lesioned strips. EFS-induced on-contractions were not different between the healthy and lesioned strips, while the non-nitrergic relaxant responses induced by EFS were decreased in the lesioned strips. The facilitating effect of prucalopride on acetylcholine release in healthy strips was similar to that reported before in macroscopically healthy colon tissue of patients with colon cancer; in lesioned strips, the effect of prucalopride was fully maintained in 6/8 patients and absent in two. Conclusions & inferences: Large intestinal endometriosis does not lead to a systematic interference with the cholinergic facilitating effect of prucalopride.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
human, large intestine, cholinergic, endometriosis, prucalopride, IDIOPATHIC CHRONIC CONSTIPATION, COLONIC CIRCULAR MUSCLE, SMOOTH-MUSCLE, NK2 RECEPTORS, CHOLINERGIC NERVES, PRUCALOPRIDE, DISEASE, BOWEL, TRANSMISSION, CONTRACTION
journal title
NEUROGASTROENTEROLOGY AND MOTILITY
Neurogastroenterol. Motil.
volume
22
issue
5
pages
557 - 563
Web of Science type
Article
Web of Science id
000276406700014
JCR category
CLINICAL NEUROLOGY
JCR impact factor
3.349 (2010)
JCR rank
49/185 (2010)
JCR quartile
2 (2010)
ISSN
1350-1925
DOI
10.1111/j.1365-2982.2009.01438.x
language
English
UGent publication?
yes
classification
A1
additional info
concluding pages: e121-e122
copyright statement
I have transferred the copyright for this publication to the publisher
id
1063773
handle
http://hdl.handle.net/1854/LU-1063773
date created
2010-10-25 18:23:18
date last changed
2010-10-26 13:24:38
@article{1063773,
  abstract     = {Background: The 5-HT4 receptor agonist prucalopride enhances large intestinal contractility by facilitating acetylcholine release through activation of 5-HT4 receptors on cholinergic nerves and is effective in patients with constipation. Patients with intestinal endometriosis can present with constipation. We investigated in vitro whether large intestinal endometriotic infiltration influences contractility and facilitation of acetylcholine release by prucalopride.
Methods: Sigmoid colon or rectum circular muscle strips were obtained at the level of an endometriotic nodule with infiltration of the Auerbach plexus, and at a macroscopically healthy site at least 5 cm cranially from the nodule, in patients undergoing laparoscopic colorectal resection because of symptomatic bowel endometriosis. Responses to muscarinic receptor stimulation and to electrical field stimulation (EFS), and the facilitating effect of prucalopride on acetylcholine release were evaluated.
Key results: The EC50 and E-max of the contractile responses to the muscarinic receptor agonist carbachol did not differ between healthy and lesioned strips. EFS-induced on-contractions were not different between the healthy and lesioned strips, while the non-nitrergic relaxant responses induced by EFS were decreased in the lesioned strips. The facilitating effect of prucalopride on acetylcholine release in healthy strips was similar to that reported before in macroscopically healthy colon tissue of patients with colon cancer; in lesioned strips, the effect of prucalopride was fully maintained in 6/8 patients and absent in two.
Conclusions \& inferences: Large intestinal endometriosis does not lead to a systematic interference with the cholinergic facilitating effect of prucalopride.},
  author       = {Lefebvre, Romain and Ferrero, S and Van Colen, Inge and Dhaese, Ingeborg and Camerini, G and Fulcheri, E and Remorgida, V},
  issn         = {1350-1925},
  journal      = {NEUROGASTROENTEROLOGY AND MOTILITY},
  keyword      = {human,large intestine,cholinergic,endometriosis,prucalopride,IDIOPATHIC CHRONIC CONSTIPATION,COLONIC CIRCULAR MUSCLE,SMOOTH-MUSCLE,NK2 RECEPTORS,CHOLINERGIC NERVES,PRUCALOPRIDE,DISEASE,BOWEL,TRANSMISSION,CONTRACTION},
  language     = {eng},
  number       = {5},
  pages        = {557--563},
  title        = {Influence of 5-HT4 receptor activation on acetylcholine release in human large intestine with endometriosis},
  url          = {http://dx.doi.org/10.1111/j.1365-2982.2009.01438.x},
  volume       = {22},
  year         = {2010},
}

Chicago
Lefebvre, Romain, S Ferrero, Inge Van Colen, Ingeborg Dhaese, G Camerini, E Fulcheri, and V Remorgida. 2010. “Influence of 5-HT4 Receptor Activation on Acetylcholine Release in Human Large Intestine with Endometriosis.” Neurogastroenterology and Motility 22 (5): 557–563.
APA
Lefebvre, R., Ferrero, S., Van Colen, I., Dhaese, I., Camerini, G., Fulcheri, E., & Remorgida, V. (2010). Influence of 5-HT4 receptor activation on acetylcholine release in human large intestine with endometriosis. NEUROGASTROENTEROLOGY AND MOTILITY, 22(5), 557–563.
Vancouver
1.
Lefebvre R, Ferrero S, Van Colen I, Dhaese I, Camerini G, Fulcheri E, et al. Influence of 5-HT4 receptor activation on acetylcholine release in human large intestine with endometriosis. NEUROGASTROENTEROLOGY AND MOTILITY. 2010;22(5):557–63.
MLA
Lefebvre, Romain, S Ferrero, Inge Van Colen, et al. “Influence of 5-HT4 Receptor Activation on Acetylcholine Release in Human Large Intestine with Endometriosis.” NEUROGASTROENTEROLOGY AND MOTILITY 22.5 (2010): 557–563. Print.