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Somatic embryogenesis and plant regeneration of tropical maize genotypes

Sylvester Anami UGent, Allan Jalemba Mgutu, Catherine Taracha, Griet Coussens UGent, Mansour Karimi UGent, Pierre Hilson UGent, Maria Van Lijsebettens UGent and Jesse Machuka (2010) PLANT CELL TISSUE AND ORGAN CULTURE. 102(3). p.285-295
abstract
In Latin America and sub-Saharan Africa, tropical maize (Zea mays L.) is a major crop for human consumption. To cope with the increasing population and changing environment, there is a need for improving tropical maize germplasm. As part of a biotechnological approach, efficient in vitro regeneration of two tropical maize inbred lines (CML216 and CML244) was established. A number of parameters were optimized, such as age of the immature embryos, plant media and growth regulator concentration. After 6 weeks of culture, somatic embryos that had already reached the coleoptilar stage produced shoots after light induction and developed into fertile plants after acclimation in the soil. The callus induction frequencies and somatic embryo-derived plantlet formation were higher when cultured with the Linsmaier and Skoog medium than those with the Chu's N6 basal medium. Regeneration of tropical maize shoots depended on the 2,4-dichlorophenoxyacetic acid (2,4-D) concentration at the callus initiation stage from immature embryos. The recalcitrance of the tropical maize inbred line TL26 to in vitro regeneration was overcome in a single-cross hybrid with the CML216 and CML244 genotypes. Remarkably, tropical maize somatic embryos were formed at the abaxial side of the scutellum facing the medium, probably from the axis of the immature embryos, as shown by histological sections. Upon co-cultivation, agrobacteria transiently expressed their intronless beta-glucuronidase-encoding gene at the embryogenic tissue, but not with an intron-containing gene, suggesting that virulence genes are induced in Agrobacterium, but that subsequent steps in the T-DNA transfer are inhibited.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
AGROBACTERIUM-MEDIATED TRANSFORMATION, Somatic embryogenesis, Callus induction, Tropical maize, ZEA-MAYS-L, TOBACCO TISSUE CULTURES, TRANSGENIC MAIZE, HOMOLOGOUS RECOMBINATION, TUMEFACIENS, DNA, EMBRYOS, CALLUS, GENES
journal title
PLANT CELL TISSUE AND ORGAN CULTURE
Plant Cell Tissue Organ Cult.
volume
102
issue
3
pages
285 - 295
Web of Science type
Article
Web of Science id
000281761900003
JCR category
PLANT SCIENCES
JCR impact factor
1.243 (2010)
JCR rank
89/185 (2010)
JCR quartile
2 (2010)
ISSN
0167-6857
DOI
10.1007/s11240-010-9731-7
language
English
UGent publication?
yes
classification
A1
copyright statement
I don't know the status of the copyright for this publication
id
1061908
handle
http://hdl.handle.net/1854/LU-1061908
date created
2010-10-21 14:23:42
date last changed
2016-12-19 15:46:43
@article{1061908,
  abstract     = {In Latin America and sub-Saharan Africa, tropical maize (Zea mays L.) is a major crop for human consumption. To cope with the increasing population and changing environment, there is a need for improving tropical maize germplasm. As part of a biotechnological approach, efficient in vitro regeneration of two tropical maize inbred lines (CML216 and CML244) was established. A number of parameters were optimized, such as age of the immature embryos, plant media and growth regulator concentration. After 6 weeks of culture, somatic embryos that had already reached the coleoptilar stage produced shoots after light induction and developed into fertile plants after acclimation in the soil. The callus induction frequencies and somatic embryo-derived plantlet formation were higher when cultured with the Linsmaier and Skoog medium than those with the Chu's N6 basal medium. Regeneration of tropical maize shoots depended on the 2,4-dichlorophenoxyacetic acid (2,4-D) concentration at the callus initiation stage from immature embryos. The recalcitrance of the tropical maize inbred line TL26 to in vitro regeneration was overcome in a single-cross hybrid with the CML216 and CML244 genotypes. Remarkably, tropical maize somatic embryos were formed at the abaxial side of the scutellum facing the medium, probably from the axis of the immature embryos, as shown by histological sections. Upon co-cultivation, agrobacteria transiently expressed their intronless beta-glucuronidase-encoding gene at the embryogenic tissue, but not with an intron-containing gene, suggesting that virulence genes are induced in Agrobacterium, but that subsequent steps in the T-DNA transfer are inhibited.},
  author       = {Anami, Sylvester and Mgutu, Allan Jalemba and Taracha, Catherine and Coussens, Griet and Karimi, Mansour and Hilson, Pierre and Van Lijsebettens, Maria and Machuka, Jesse},
  issn         = {0167-6857},
  journal      = {PLANT CELL TISSUE AND ORGAN CULTURE},
  keyword      = {AGROBACTERIUM-MEDIATED TRANSFORMATION,Somatic embryogenesis,Callus induction,Tropical maize,ZEA-MAYS-L,TOBACCO TISSUE CULTURES,TRANSGENIC MAIZE,HOMOLOGOUS RECOMBINATION,TUMEFACIENS,DNA,EMBRYOS,CALLUS,GENES},
  language     = {eng},
  number       = {3},
  pages        = {285--295},
  title        = {Somatic embryogenesis and plant regeneration of tropical maize genotypes},
  url          = {http://dx.doi.org/10.1007/s11240-010-9731-7},
  volume       = {102},
  year         = {2010},
}

Chicago
Anami, Sylvester, Allan Jalemba Mgutu, Catherine Taracha, Griet Coussens, Mansour Karimi, Pierre Hilson, Maria Van Lijsebettens, and Jesse Machuka. 2010. “Somatic Embryogenesis and Plant Regeneration of Tropical Maize Genotypes.” Plant Cell Tissue and Organ Culture 102 (3): 285–295.
APA
Anami, S., Mgutu, A. J., Taracha, C., Coussens, G., Karimi, M., Hilson, P., Van Lijsebettens, M., et al. (2010). Somatic embryogenesis and plant regeneration of tropical maize genotypes. PLANT CELL TISSUE AND ORGAN CULTURE, 102(3), 285–295.
Vancouver
1.
Anami S, Mgutu AJ, Taracha C, Coussens G, Karimi M, Hilson P, et al. Somatic embryogenesis and plant regeneration of tropical maize genotypes. PLANT CELL TISSUE AND ORGAN CULTURE. 2010;102(3):285–95.
MLA
Anami, Sylvester, Allan Jalemba Mgutu, Catherine Taracha, et al. “Somatic Embryogenesis and Plant Regeneration of Tropical Maize Genotypes.” PLANT CELL TISSUE AND ORGAN CULTURE 102.3 (2010): 285–295. Print.