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Vegf regulates embryonic erythroid development through Gata1 modulation

Benjamin Drogat UGent, Joanna Kalucka, Laura Gutierrez, Hamida Hammad UGent, Steven Goossens UGent, Morvarid Farhang Ghahremani UGent, Sona Bartunkova UGent, Katharina Haigh UGent, Kim Deswarte UGent and Omar Nyabi, et al. (2010) BLOOD. 116(12). p.2141-2151
abstract
To determine the role of vascular endothelial growth factor (Vegf) in embryonic erythroid development we have deleted or overexpressed Vegf specifically in the erythroid lineage using the EpoR-iCre transgenic line in combination with Cre/ loxP conditional gain and loss of function Vegf alleles. ROSA26 promoter-based expression of the Vegf164 isoform in the early erythroid lineage resulted in a differentiation block of primitive erythroid progenitor (EryP) development and a partial block in definitive erythropoiesis between the erythroid burst-forming unit and erythroid colony-forming unit stages. Decreased mRNA expression levels of the key erythroid transcription factor Gata1 were causally linked to this phenotype. Conditional deletion of Vegf within the erythroid lineage was associated with increased Gata1 levels and increased erythroid differentiation. Expression of a ROSA26-based GATA2 transgene rescued Gata1 mRNAlevels and target genes and restored erythroid differentiation in our Vegf gain of function model. These results demonstrate that Vegf modulates Gata1 expression levels in vivo and provides new molecular insight into Vegf’s ability to modulate erythropoiesis.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
HEMATOPOIETIC STEM-CELLS, DEFINITIVE HEMATOPOIESIS, TRANSCRIPTION FACTOR GATA-1, GENE-EXPRESSION, YOLK-SAC, MOUSE, DIFFERENTIATION, ERYTHROPOIETIN, SURVIVAL, LINEAGE
journal title
BLOOD
Blood
volume
116
issue
12
pages
2141 - 2151
Web of Science type
Article
Web of Science id
000282137300023
JCR category
HEMATOLOGY
JCR impact factor
10.558 (2010)
JCR rank
2/65 (2010)
JCR quartile
1 (2010)
ISSN
0006-4971
DOI
10.1182/blood-2010-01-264143
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1060090
handle
http://hdl.handle.net/1854/LU-1060090
date created
2010-10-18 14:30:21
date last changed
2012-06-26 14:32:09
@article{1060090,
  abstract     = {To determine the role of vascular endothelial growth factor (Vegf) in embryonic erythroid development we have deleted or overexpressed Vegf specifically in the erythroid lineage using the EpoR-iCre transgenic line in combination with Cre/ loxP conditional gain and loss of function Vegf alleles. ROSA26 promoter-based expression of the Vegf164 isoform in the early erythroid lineage resulted in a differentiation block of primitive erythroid progenitor (EryP) development and a partial block in definitive erythropoiesis between the erythroid burst-forming unit and erythroid colony-forming unit stages. Decreased mRNA expression levels of the key erythroid transcription factor Gata1 were causally linked to this phenotype. Conditional deletion of Vegf within the erythroid lineage was associated with increased Gata1 levels and increased erythroid differentiation. Expression of a ROSA26-based GATA2 transgene rescued Gata1 mRNAlevels and target genes and restored erythroid differentiation in our Vegf gain of function model. These results demonstrate that Vegf modulates Gata1 expression levels in vivo and provides new molecular insight into Vegf{\textquoteright}s ability to modulate erythropoiesis.},
  author       = {Drogat, Benjamin and Kalucka, Joanna and Gutierrez, Laura and Hammad, Hamida and Goossens, Steven and Farhang Ghahremani, Morvarid and Bartunkova, Sona and Haigh, Katharina and Deswarte, Kim and Nyabi, Omar and Naessens, Micha{\"e}l and Ferrara, Napoleone and Klingmuller, Uursula and Lambrecht, Bart and Nagy, Andras and Philipsen, Sjaak and Haigh, Jody},
  issn         = {0006-4971},
  journal      = {BLOOD},
  keyword      = {HEMATOPOIETIC STEM-CELLS,DEFINITIVE HEMATOPOIESIS,TRANSCRIPTION FACTOR GATA-1,GENE-EXPRESSION,YOLK-SAC,MOUSE,DIFFERENTIATION,ERYTHROPOIETIN,SURVIVAL,LINEAGE},
  language     = {eng},
  number       = {12},
  pages        = {2141--2151},
  title        = {Vegf regulates embryonic erythroid development through Gata1 modulation},
  url          = {http://dx.doi.org/10.1182/blood-2010-01-264143},
  volume       = {116},
  year         = {2010},
}

Chicago
Drogat, Benjamin, Joanna Kalucka, Laura Gutierrez, Hamida Hammad, Steven Goossens, Morvarid Farhang Ghahremani, Sona Bartunkova, et al. 2010. “Vegf Regulates Embryonic Erythroid Development Through Gata1 Modulation.” Blood 116 (12): 2141–2151.
APA
Drogat, B., Kalucka, J., Gutierrez, L., Hammad, H., Goossens, S., Farhang Ghahremani, M., Bartunkova, S., et al. (2010). Vegf regulates embryonic erythroid development through Gata1 modulation. BLOOD, 116(12), 2141–2151.
Vancouver
1.
Drogat B, Kalucka J, Gutierrez L, Hammad H, Goossens S, Farhang Ghahremani M, et al. Vegf regulates embryonic erythroid development through Gata1 modulation. BLOOD. 2010;116(12):2141–51.
MLA
Drogat, Benjamin, Joanna Kalucka, Laura Gutierrez, et al. “Vegf Regulates Embryonic Erythroid Development Through Gata1 Modulation.” BLOOD 116.12 (2010): 2141–2151. Print.