Advanced search
1 file | 1.49 MB

Vegf regulates embryonic erythroid development through Gata1 modulation

(2010) BLOOD. 116(12). p.2141-2151
Author
Organization
Abstract
To determine the role of vascular endothelial growth factor (Vegf) in embryonic erythroid development we have deleted or overexpressed Vegf specifically in the erythroid lineage using the EpoR-iCre transgenic line in combination with Cre/ loxP conditional gain and loss of function Vegf alleles. ROSA26 promoter-based expression of the Vegf164 isoform in the early erythroid lineage resulted in a differentiation block of primitive erythroid progenitor (EryP) development and a partial block in definitive erythropoiesis between the erythroid burst-forming unit and erythroid colony-forming unit stages. Decreased mRNA expression levels of the key erythroid transcription factor Gata1 were causally linked to this phenotype. Conditional deletion of Vegf within the erythroid lineage was associated with increased Gata1 levels and increased erythroid differentiation. Expression of a ROSA26-based GATA2 transgene rescued Gata1 mRNAlevels and target genes and restored erythroid differentiation in our Vegf gain of function model. These results demonstrate that Vegf modulates Gata1 expression levels in vivo and provides new molecular insight into Vegf’s ability to modulate erythropoiesis.
Keywords
HEMATOPOIETIC STEM-CELLS, DEFINITIVE HEMATOPOIESIS, TRANSCRIPTION FACTOR GATA-1, GENE-EXPRESSION, YOLK-SAC, MOUSE, DIFFERENTIATION, ERYTHROPOIETIN, SURVIVAL, LINEAGE

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 1.49 MB

Citation

Please use this url to cite or link to this publication:

Chicago
Drogat, Benjamin, Joanna Kalucka, Laura Gutierrez, Hamida Hammad, Steven Goossens, Morvarid Farhang Ghahremani, Sona Bartunkova, et al. 2010. “Vegf Regulates Embryonic Erythroid Development Through Gata1 Modulation.” Blood 116 (12): 2141–2151.
APA
Drogat, B., Kalucka, J., Gutierrez, L., Hammad, H., Goossens, S., Farhang Ghahremani, M., Bartunkova, S., et al. (2010). Vegf regulates embryonic erythroid development through Gata1 modulation. BLOOD, 116(12), 2141–2151.
Vancouver
1.
Drogat B, Kalucka J, Gutierrez L, Hammad H, Goossens S, Farhang Ghahremani M, et al. Vegf regulates embryonic erythroid development through Gata1 modulation. BLOOD. 2010;116(12):2141–51.
MLA
Drogat, Benjamin, Joanna Kalucka, Laura Gutierrez, et al. “Vegf Regulates Embryonic Erythroid Development Through Gata1 Modulation.” BLOOD 116.12 (2010): 2141–2151. Print.
@article{1060090,
  abstract     = {To determine the role of vascular endothelial growth factor (Vegf) in embryonic erythroid development we have deleted or overexpressed Vegf specifically in the erythroid lineage using the EpoR-iCre transgenic line in combination with Cre/ loxP conditional gain and loss of function Vegf alleles. ROSA26 promoter-based expression of the Vegf164 isoform in the early erythroid lineage resulted in a differentiation block of primitive erythroid progenitor (EryP) development and a partial block in definitive erythropoiesis between the erythroid burst-forming unit and erythroid colony-forming unit stages. Decreased mRNA expression levels of the key erythroid transcription factor Gata1 were causally linked to this phenotype. Conditional deletion of Vegf within the erythroid lineage was associated with increased Gata1 levels and increased erythroid differentiation. Expression of a ROSA26-based GATA2 transgene rescued Gata1 mRNAlevels and target genes and restored erythroid differentiation in our Vegf gain of function model. These results demonstrate that Vegf modulates Gata1 expression levels in vivo and provides new molecular insight into Vegf{\textquoteright}s ability to modulate erythropoiesis.},
  author       = {Drogat, Benjamin and Kalucka, Joanna and Gutierrez, Laura and Hammad, Hamida and Goossens, Steven and Farhang Ghahremani, Morvarid and Bartunkova, Sona and Haigh, Katharina and Deswarte, Kim and Nyabi, Omar and Naessens, Micha{\"e}l and Ferrara, Napoleone and Klingmuller, Uursula and Lambrecht, Bart and Nagy, Andras and Philipsen, Sjaak and Haigh, Jody},
  issn         = {0006-4971},
  journal      = {BLOOD},
  keyword      = {HEMATOPOIETIC STEM-CELLS,DEFINITIVE HEMATOPOIESIS,TRANSCRIPTION FACTOR GATA-1,GENE-EXPRESSION,YOLK-SAC,MOUSE,DIFFERENTIATION,ERYTHROPOIETIN,SURVIVAL,LINEAGE},
  language     = {eng},
  number       = {12},
  pages        = {2141--2151},
  title        = {Vegf regulates embryonic erythroid development through Gata1 modulation},
  url          = {http://dx.doi.org/10.1182/blood-2010-01-264143},
  volume       = {116},
  year         = {2010},
}

Altmetric
View in Altmetric
Web of Science
Times cited: