Advanced search
1 file | 3.13 MB Add to list

Prevention and eradication of microbial biofilms

(2010)
Author
Promoter
(UGent) and (UGent)
Organization
Abstract
Medical materials are frequently introduced in the human body. They consist of biocompatible polymers such as PDMS, to which micro-organisms easily adhere, resulting in the formation of biofilms. The first antibiofilm strategy was to try to prevent Candida albicans biofilm formation on PDMS. To this end two approaches were used, i.e. the modification of the PDMS surface by grafting of antimicrobial compounds and the incorporation of antimycotics in the bulk PDMS polymer. DMAEMA was covalently bound to PDMS and subsequently quaternized at the surface with alkyl chains of different length. Shorter alkyl chains yielded substantially lower C. albicans sessile cell counts in a dynamic biofilm model system (MRD) than longer ones. PEI grafted on PMMA or PDMS showed poor efficacy as to the inhibition of C. albicans biofilm development. Finally, PDMS was functionalized with cationic peptides. Dhvar 4-PDMS and Poly-D-lysine-PDMS (active moieties with molecular weight distributions of 1,000-4,000 and 4,000-15,000, respectively) yielded the highest reduction (> 90%) in the number of C. albicans biofilm cells. In a second experimental approach, we incorporated miconazole, nystatin, tea tree oil and zinc pyrithione in the PDMS polymer. Impregnated PDMS disks (prepared by immersion in a solution containing the antimycotic) yielded reductions in sessile cell counts of more than 90%. When the antimycotic was mixed with the PDMS compounds prior to vulcanization, an antibiofilm effect was only observed in a microtiter plate, due to the rapid release (burst effect) of the active compound in the small volume of a well. In the MRD, the antimycotic released from either an impregnated or medicated disk is washed away by the liquid flow. In a second antibiofilm strategy entitled “biofilm eradication”, the aim was to study the ability of NitrAdineTM, a disinfectant for oral hygiene to remove biofilms from polymeric materials. Under various conditions NitrAdineTM showed high activity (> 3 log units reduction) against biofilms of C. albicans and S. aureus, grown on PMMA disks in the MRD.
Keywords
antibiofilm strategy, Candida albicans, biofilm

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 3.13 MB

Citation

Please use this url to cite or link to this publication:

MLA
De Prijck, Kristof. Prevention and Eradication of Microbial Biofilms. Ghent University. Faculty of Pharmaceutical Sciences, 2010.
APA
De Prijck, K. (2010). Prevention and eradication of microbial biofilms. Ghent University. Faculty of Pharmaceutical Sciences, Ghent, Belgium.
Chicago author-date
De Prijck, Kristof. 2010. “Prevention and Eradication of Microbial Biofilms.” Ghent, Belgium: Ghent University. Faculty of Pharmaceutical Sciences.
Chicago author-date (all authors)
De Prijck, Kristof. 2010. “Prevention and Eradication of Microbial Biofilms.” Ghent, Belgium: Ghent University. Faculty of Pharmaceutical Sciences.
Vancouver
1.
De Prijck K. Prevention and eradication of microbial biofilms. [Ghent, Belgium]: Ghent University. Faculty of Pharmaceutical Sciences; 2010.
IEEE
[1]
K. De Prijck, “Prevention and eradication of microbial biofilms,” Ghent University. Faculty of Pharmaceutical Sciences, Ghent, Belgium, 2010.
@phdthesis{1059292,
  abstract     = {{Medical materials are frequently introduced in the human body. They consist of biocompatible polymers such as PDMS, to which micro-organisms easily adhere, resulting in the formation of biofilms. 
The first antibiofilm strategy was to try to prevent Candida albicans biofilm formation on PDMS. To this end two approaches were used, i.e. the modification of the PDMS surface by grafting of antimicrobial compounds and the incorporation of antimycotics in the bulk PDMS polymer. DMAEMA was covalently bound to PDMS and subsequently quaternized at the surface with alkyl chains of different length. Shorter alkyl chains yielded substantially lower C. albicans sessile cell counts in a dynamic biofilm model system (MRD) than longer ones. PEI grafted on PMMA or PDMS showed poor efficacy as to the inhibition of C. albicans biofilm development. Finally, PDMS was functionalized with cationic peptides. Dhvar 4-PDMS and Poly-D-lysine-PDMS (active moieties with molecular weight distributions of 1,000-4,000 and 4,000-15,000, respectively) yielded the highest reduction (> 90%) in the number of C. albicans biofilm cells. In a second experimental approach, we incorporated miconazole, nystatin, tea tree oil and zinc pyrithione in the PDMS polymer. Impregnated PDMS disks (prepared by immersion in a solution containing the antimycotic) yielded reductions in sessile cell counts of more than 90%. When the antimycotic was mixed with the PDMS compounds prior to vulcanization, an antibiofilm effect was only observed in a microtiter plate, due to the rapid release (burst effect) of the active compound in the small volume of a well. In the MRD, the antimycotic released from either an impregnated or medicated disk is washed away by the liquid flow. 
In a second antibiofilm strategy entitled “biofilm eradication”, the aim was to study the ability of NitrAdineTM, a disinfectant for oral hygiene to remove biofilms from polymeric materials. Under various conditions NitrAdineTM showed high activity (> 3 log units reduction) against biofilms of C. albicans and S. aureus, grown on PMMA disks in the MRD.}},
  author       = {{De Prijck, Kristof}},
  keywords     = {{antibiofilm strategy,Candida albicans,biofilm}},
  language     = {{eng}},
  pages        = {{156 + annexes}},
  publisher    = {{Ghent University. Faculty of Pharmaceutical Sciences}},
  school       = {{Ghent University}},
  title        = {{Prevention and eradication of microbial biofilms}},
  url          = {{http://lib.ugent.be/fulltxt/RUG01/001/431/090/RUG01-001431090_2010_0001_AC.pdf}},
  year         = {{2010}},
}