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High efficiency protoplast isolation from in vitro cultures and hairy roots of Maesa lanceolata

Ellen Lambert UGent and Danny Geelen UGent (2010) AFRICAN JOURNAL OF BIOTECHNOLOGY. 9(42). p.7071-7078
abstract
In vitro cultures of the medicinal plant Maesa lanceolata were established to enable the cultivation of plant material for the production of protoplasts. Callus cultures were initiated using leaves collected from shoot cultures and the root tips from hairy root cultures obtained upon Agrobacterium rhizogenes transformation. For the isolation of protoplast, the different explant material of M. lanceolata was exposed to an enzyme mixture consisting of 1.5% cellulase, 0.5% macerozyme R-10 and 0.5 M mannitol. About 6 x 106 protoplasts g-1 fresh weight were obtained from leaf material and 5 x 105 protoplasts g-1 fresh weight from callus. To obtain high amounts of hairy root protoplasts, the cultures were pretreated with the auxin indole-3-butyric acid (IBA) that stimulated the formation of novel root tips. Using the dissected root tips as starting material, 8 x 105 protoplasts g-1 fresh weight were obtained per preparation. The protoplast isolation method will enable further studies on the transformation and fusion of protoplasts from M. lanceolata.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
protoplast isolation., Maesa lanceolata, tissue culture, in vitro conservation, TRITERPENOID SAPONINS, BIOLOGICAL-ACTIVITIES, HYOSCYAMUS MUTICUS, PLANT-REGENERATION, TRANSGENIC PLANTS, TISSUE CULTURES, SOMATIC HYBRIDS, TRANSFORMATION, CALLUS, GENE
journal title
AFRICAN JOURNAL OF BIOTECHNOLOGY
Afr. J. Biotechnol.
volume
9
issue
42
pages
7071 - 7078
Web of Science type
Article
Web of Science id
000283197400009
JCR category
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
JCR impact factor
0.573 (2010)
JCR rank
137/158 (2010)
JCR quartile
4 (2010)
ISSN
1684-5315
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1057768
handle
http://hdl.handle.net/1854/LU-1057768
date created
2010-10-12 10:06:05
date last changed
2016-12-19 15:46:27
@article{1057768,
  abstract     = {In vitro cultures of the medicinal plant Maesa lanceolata were established to enable the cultivation of plant material for the production of protoplasts. Callus cultures were initiated using leaves collected from shoot cultures and the root tips from hairy root cultures obtained upon Agrobacterium rhizogenes transformation. For the isolation of protoplast, the different explant material of M. lanceolata was exposed to an enzyme mixture consisting of 1.5\% cellulase, 0.5\% macerozyme R-10 and 0.5 M mannitol.
About 6 x 106 protoplasts g-1 fresh weight were obtained from leaf material and 5 x 105 protoplasts g-1 fresh weight from callus. To obtain high amounts of hairy root protoplasts, the cultures were pretreated with the auxin indole-3-butyric acid (IBA) that stimulated the formation of novel root tips. Using the dissected root tips as starting material, 8 x 105 protoplasts g-1 fresh weight were obtained per preparation. The protoplast isolation method will enable further studies on the transformation and fusion of protoplasts from M. lanceolata.},
  author       = {Lambert, Ellen and Geelen, Danny},
  issn         = {1684-5315},
  journal      = {AFRICAN JOURNAL OF BIOTECHNOLOGY},
  keyword      = {protoplast isolation.,Maesa lanceolata,tissue culture,in vitro conservation,TRITERPENOID SAPONINS,BIOLOGICAL-ACTIVITIES,HYOSCYAMUS MUTICUS,PLANT-REGENERATION,TRANSGENIC PLANTS,TISSUE CULTURES,SOMATIC HYBRIDS,TRANSFORMATION,CALLUS,GENE},
  language     = {eng},
  number       = {42},
  pages        = {7071--7078},
  title        = {High efficiency protoplast isolation from in vitro cultures and hairy roots of Maesa lanceolata},
  volume       = {9},
  year         = {2010},
}

Chicago
Lambert, Ellen, and Danny Geelen. 2010. “High Efficiency Protoplast Isolation from in Vitro Cultures and Hairy Roots of Maesa Lanceolata.” African Journal of Biotechnology 9 (42): 7071–7078.
APA
Lambert, Ellen, & Geelen, D. (2010). High efficiency protoplast isolation from in vitro cultures and hairy roots of Maesa lanceolata. AFRICAN JOURNAL OF BIOTECHNOLOGY, 9(42), 7071–7078.
Vancouver
1.
Lambert E, Geelen D. High efficiency protoplast isolation from in vitro cultures and hairy roots of Maesa lanceolata. AFRICAN JOURNAL OF BIOTECHNOLOGY. 2010;9(42):7071–8.
MLA
Lambert, Ellen, and Danny Geelen. “High Efficiency Protoplast Isolation from in Vitro Cultures and Hairy Roots of Maesa Lanceolata.” AFRICAN JOURNAL OF BIOTECHNOLOGY 9.42 (2010): 7071–7078. Print.