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Expression of artificial microRNAs in Physcomitrella patens

Isam Fattash, Basel Khraiwesh UGent, M Asif Arif and Wolfgang Frank (2012) Transgenic plants : methods and protocols. In Methods in Molecular Biology 847. p.293-315
abstract
MicroRNAs (miRNAs) are ~21nt long small RNAs transcribed from endogenous MIR genes which form precursor RNAs with a characteristic hairpin structure. MiRNAs control the expression of cognate target genes by binding to reverse complementary sequences resulting in cleavage or translational inhibition of the target RNA. Artificial miRNAs (amiRNAs) can be generated by exchanging the miRNA/miRNA* sequence of endogenous MIR precursor genes, while maintaining the general pattern of matches and mismatches in the foldback. Thus, for functional gene analysis amiRNAs can be designed to target any gene of interest.
Please use this url to cite or link to this publication:
author
organization
year
type
bookChapter
publication status
published
subject
keyword
Physcomitrella patens, artificial microRNA, gene knockdown, microRNA, RNA interference
book title
Transgenic plants : methods and protocols
editor
Jim M Dunwell and Andy C Wetten
series title
Methods in Molecular Biology
volume
847
edition
2nd ed.
pages
293 - 315
publisher
Springer
place of publication
New York, NY, USA
ISSN
1064-3745
ISBN
9781617795589
9781617795572
DOI
10.1007/978-1-61779-558-9_25
language
English
UGent publication?
yes
classification
B2
copyright statement
I have transferred the copyright for this publication to the publisher
id
1057749
handle
http://hdl.handle.net/1854/LU-1057749
date created
2010-10-12 09:57:07
date last changed
2017-03-23 10:08:01
@incollection{1057749,
  abstract     = {MicroRNAs (miRNAs) are {\texttildelow}21nt long small RNAs transcribed from endogenous MIR genes which form precursor RNAs with a characteristic hairpin structure. MiRNAs control the expression of cognate target genes by binding to reverse complementary sequences resulting in cleavage or translational inhibition of the target RNA. Artificial miRNAs (amiRNAs) can be generated by exchanging the miRNA/miRNA* sequence of endogenous MIR precursor genes, while maintaining the general pattern of matches and mismatches in the foldback. Thus, for functional gene analysis amiRNAs can be designed to target any gene of interest.},
  author       = {Fattash, Isam and Khraiwesh, Basel and Arif, M Asif  and Frank, Wolfgang },
  booktitle    = {Transgenic plants : methods and protocols},
  editor       = {Dunwell, Jim  M and Wetten, Andy C},
  isbn         = {9781617795589},
  issn         = {1064-3745},
  keyword      = {Physcomitrella patens,artificial microRNA,gene knockdown,microRNA,RNA interference},
  language     = {eng},
  pages        = {293--315},
  publisher    = {Springer},
  series       = {Methods in Molecular Biology},
  title        = {Expression of artificial microRNAs in Physcomitrella patens},
  url          = {http://dx.doi.org/10.1007/978-1-61779-558-9\_25},
  volume       = {847},
  year         = {2012},
}

Chicago
Fattash, Isam, Basel Khraiwesh, M Asif Arif, and Wolfgang Frank. 2012. “Expression of Artificial microRNAs in Physcomitrella Patens.” In Transgenic Plants : Methods and Protocols, ed. Jim  M Dunwell and Andy C Wetten, 847:293–315. 2nd ed. New York, NY, USA: Springer.
APA
Fattash, I., Khraiwesh, B., Arif, M. A., & Frank, W. (2012). Expression of artificial microRNAs in Physcomitrella patens. In J. M. Dunwell & A. C. Wetten (Eds.), Transgenic plants : methods and protocols (2nd ed., Vol. 847, pp. 293–315). New York, NY, USA: Springer.
Vancouver
1.
Fattash I, Khraiwesh B, Arif MA, Frank W. Expression of artificial microRNAs in Physcomitrella patens. In: Dunwell JM, Wetten AC, editors. Transgenic plants : methods and protocols. 2nd ed. New York, NY, USA: Springer; 2012. p. 293–315.
MLA
Fattash, Isam, Basel Khraiwesh, M Asif Arif, et al. “Expression of Artificial microRNAs in Physcomitrella Patens.” Transgenic Plants : Methods and Protocols. 2nd ed. Ed. Jim  M Dunwell & Andy C Wetten. Vol. 847. New York, NY, USA: Springer, 2012. 293–315. Print.