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Expression of artificial microRNAs in Physcomitrella patens

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Abstract
MicroRNAs (miRNAs) are ~21nt long small RNAs transcribed from endogenous MIR genes which form precursor RNAs with a characteristic hairpin structure. MiRNAs control the expression of cognate target genes by binding to reverse complementary sequences resulting in cleavage or translational inhibition of the target RNA. Artificial miRNAs (amiRNAs) can be generated by exchanging the miRNA/miRNA* sequence of endogenous MIR precursor genes, while maintaining the general pattern of matches and mismatches in the foldback. Thus, for functional gene analysis amiRNAs can be designed to target any gene of interest.
Keywords
Physcomitrella patens, artificial microRNA, gene knockdown, microRNA, RNA interference

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Chicago
Fattash, Isam, Basel Khraiwesh, M Asif Arif, and Wolfgang Frank. 2012. “Expression of Artificial microRNAs in Physcomitrella Patens.” In Transgenic Plants : Methods and Protocols, ed. Jim  M Dunwell and Andy C Wetten, 847:293–315. 2nd ed. New York, NY, USA: Springer.
APA
Fattash, I., Khraiwesh, B., Arif, M. A., & Frank, W. (2012). Expression of artificial microRNAs in Physcomitrella patens. In J. M. Dunwell & A. C. Wetten (Eds.), Transgenic plants : methods and protocols (2nd ed., Vol. 847, pp. 293–315). New York, NY, USA: Springer.
Vancouver
1.
Fattash I, Khraiwesh B, Arif MA, Frank W. Expression of artificial microRNAs in Physcomitrella patens. In: Dunwell JM, Wetten AC, editors. Transgenic plants : methods and protocols. 2nd ed. New York, NY, USA: Springer; 2012. p. 293–315.
MLA
Fattash, Isam, Basel Khraiwesh, M Asif Arif, et al. “Expression of Artificial microRNAs in Physcomitrella Patens.” Transgenic Plants : Methods and Protocols. 2nd ed. Ed. Jim  M Dunwell & Andy C Wetten. Vol. 847. New York, NY, USA: Springer, 2012. 293–315. Print.
@incollection{1057749,
  abstract     = {MicroRNAs (miRNAs) are {\texttildelow}21nt long small RNAs transcribed from endogenous MIR genes which form precursor RNAs with a characteristic hairpin structure. MiRNAs control the expression of cognate target genes by binding to reverse complementary sequences resulting in cleavage or translational inhibition of the target RNA. Artificial miRNAs (amiRNAs) can be generated by exchanging the miRNA/miRNA* sequence of endogenous MIR precursor genes, while maintaining the general pattern of matches and mismatches in the foldback. Thus, for functional gene analysis amiRNAs can be designed to target any gene of interest.},
  author       = {Fattash, Isam and Khraiwesh, Basel and Arif, M Asif  and Frank, Wolfgang },
  booktitle    = {Transgenic plants : methods and protocols},
  editor       = {Dunwell, Jim  M and Wetten, Andy C},
  isbn         = {9781617795589},
  issn         = {1064-3745},
  keyword      = {Physcomitrella patens,artificial microRNA,gene knockdown,microRNA,RNA interference},
  language     = {eng},
  pages        = {293--315},
  publisher    = {Springer},
  series       = {Methods in Molecular Biology},
  title        = {Expression of artificial microRNAs in Physcomitrella patens},
  url          = {http://dx.doi.org/10.1007/978-1-61779-558-9\_25},
  volume       = {847},
  year         = {2012},
}

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