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Use of Northern blotting for specific detection of small RNA molecules in transgenic plants

Basel Khraiwesh (UGent)
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Abstract
Small RNAs (20-24 nucleotides long and non protein coding) have been increasingly investigated. They were responsible for phenomena described as RNA interference (RNAi), co-suppression, gene silencing or quelling. Major classes of small RNAs include MicroRNAs (miRNAs) and small interfering RNAs (siRNAs), which differ in their biosynthesis. MiRNAs control the expression of cognate target genes by binding to reverse complementary sequences resulting in cleavage or translational inhibition of the target RNA. SiRNAs have similar structure, function and biogenesis as miRNAs, siRNAs derive from long double stranded RNA of transgenes, endogenous repeat sequences or transposons. Understanding these fundamental processes requires the sensitive and specific detection of small RNA species. In this report, we present a simple northern blot protocol for small RNAs in transgenic plants.
Keywords
small RNA, miRNA, siRNA, blotting, northern, transgenic plants.

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Please use this url to cite or link to this publication:

Chicago
Khraiwesh, Basel. 2012. “Use of Northern Blotting for Specific Detection of Small RNA Molecules in Transgenic Plants.” In Transgenic Plants : Methods and Protocols, ed. Jim  M Dunwell and Andy C Wetten, 847:25–32. 2nd ed. New York, NY, USA: Springer.
APA
Khraiwesh, B. (2012). Use of Northern blotting for specific detection of small RNA molecules in transgenic plants. In J. M. Dunwell & A. C. Wetten (Eds.), Transgenic plants : methods and protocols (2nd ed., Vol. 847, pp. 25–32). New York, NY, USA: Springer.
Vancouver
1.
Khraiwesh B. Use of Northern blotting for specific detection of small RNA molecules in transgenic plants. In: Dunwell JM, Wetten AC, editors. Transgenic plants : methods and protocols. 2nd ed. New York, NY, USA: Springer; 2012. p. 25–32.
MLA
Khraiwesh, Basel. “Use of Northern Blotting for Specific Detection of Small RNA Molecules in Transgenic Plants.” Transgenic Plants : Methods and Protocols. 2nd ed. Ed. Jim  M Dunwell & Andy C Wetten. Vol. 847. New York, NY, USA: Springer, 2012. 25–32. Print.
@incollection{1057691,
  abstract     = {Small RNAs (20-24 nucleotides long and non protein coding) have been increasingly investigated. They were responsible for phenomena described as RNA interference (RNAi), co-suppression, gene silencing or quelling. Major classes of small RNAs include MicroRNAs (miRNAs) and small interfering RNAs (siRNAs), which differ in their biosynthesis. MiRNAs control the expression of cognate target genes by binding to reverse complementary sequences resulting in cleavage or translational inhibition of the target RNA. SiRNAs have similar structure, function and biogenesis as miRNAs, siRNAs derive from long double stranded RNA of transgenes, endogenous repeat sequences or transposons. Understanding these fundamental processes requires the sensitive and specific detection of small RNA species. In this report, we present a simple northern blot protocol for small RNAs in transgenic plants.},
  author       = {Khraiwesh, Basel},
  booktitle    = {Transgenic plants : methods and protocols},
  editor       = {Dunwell, Jim  M and Wetten, Andy C},
  isbn         = {9781617795572},
  issn         = {1064-3745},
  keywords     = {small RNA,miRNA,siRNA,blotting,northern,transgenic plants.},
  language     = {eng},
  pages        = {25--32},
  publisher    = {Springer},
  series       = {Methods in Molecular Biology},
  title        = {Use of Northern blotting for specific detection of small RNA molecules in transgenic plants},
  url          = {http://dx.doi.org/10.1007/978-1-61779-558-9_3},
  volume       = {847},
  year         = {2012},
}

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