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PHB-degrading bacteria isolated from the gastrointestinal tract of aquatic animals as protective actors against luminescent vibriosis

Yiying Liu (UGent) , Peter De Schryver (UGent) , Bart Van Delsen (UGent) , Loïs Maignien (UGent) , Nico Boon (UGent) , Patrick Sorgeloos (UGent) , Willy Verstraete (UGent) , Peter Bossier (UGent) and Tom Defoirdt (UGent)
(2010) FEMS MICROBIOLOGY ECOLOGY. 74(1). p.196-204
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Abstract
The use of poly-beta-hydroxybutyrate (PHB) was shown to be successful in increasing the resistance of brine shrimp against pathogenic infections. In this study, we isolated for the first time PHB-degrading bacteria from a gastrointestinal environment. Pure strains of PHB-degrading bacteria were isolated from Siberian sturgeon, European sea bass and giant river prawn. The capability of selected isolates to degrade PHB was confirmed in at least two of three setups: (1) growth in minimal medium containing PHB as the sole carbon (C) source, (2) production of clearing zones on minimal agar containing PHB as the sole C source and (3) degradation of PHB (as determined by HPLC analysis) in 10% Luria-Bertani medium containing PHB. Challenge tests showed that the PHB-degrading activity of the selected isolates increased the survival of brine shrimp larvae challenged to a pathogenic Vibrio campbellii strain by a factor 2-3. Finally, one of the PHB-degrading isolates from sturgeon showed a double biocontrol effect because it was also able to inactivate acylhomoserine lactones, a type of quorum-sensing molecule that regulates the virulence of different pathogenic bacteria. Thus, the combined supplementation of a PHB-degrading bacterium and PHB as a synbioticum provides perspectives for improving the gastrointestinal health of aquatic animals.
Keywords
PHB, polyhydroxyalkanoate, depolymerization, brine shrimp, probiotic, aquaculture, HYDROXYBUTYRATE-ACCUMULATING BACTERIA, GRADIENT GEL-ELECTROPHORESIS, QUORUM-SENSING SIGNALS, ARTEMIA-FRANCISCANA, MICROBIAL-DEGRADATION, AQUACULTURE, ANTIBIOTICS, INFECTIONS, CAMPBELLII, HARVEYI

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Citation

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Chicago
Liu, Yiying, Peter De Schryver, Bart Van Delsen, Loïs Maignien, Nico Boon, Patrick Sorgeloos, Willy Verstraete, Peter Bossier, and Tom Defoirdt. 2010. “PHB-degrading Bacteria Isolated from the Gastrointestinal Tract of Aquatic Animals as Protective Actors Against Luminescent Vibriosis.” Fems Microbiology Ecology 74 (1): 196–204.
APA
Liu, Yiying, De Schryver, P., Van Delsen, B., Maignien, L., Boon, N., Sorgeloos, P., Verstraete, W., et al. (2010). PHB-degrading bacteria isolated from the gastrointestinal tract of aquatic animals as protective actors against luminescent vibriosis. FEMS MICROBIOLOGY ECOLOGY, 74(1), 196–204.
Vancouver
1.
Liu Y, De Schryver P, Van Delsen B, Maignien L, Boon N, Sorgeloos P, et al. PHB-degrading bacteria isolated from the gastrointestinal tract of aquatic animals as protective actors against luminescent vibriosis. FEMS MICROBIOLOGY ECOLOGY. 2010;74(1):196–204.
MLA
Liu, Yiying, Peter De Schryver, Bart Van Delsen, et al. “PHB-degrading Bacteria Isolated from the Gastrointestinal Tract of Aquatic Animals as Protective Actors Against Luminescent Vibriosis.” FEMS MICROBIOLOGY ECOLOGY 74.1 (2010): 196–204. Print.
@article{1056758,
  abstract     = {The use of poly-beta-hydroxybutyrate (PHB) was shown to be successful in increasing the resistance of brine shrimp against pathogenic infections. In this study, we isolated for the first time PHB-degrading bacteria from a gastrointestinal environment. Pure strains of PHB-degrading bacteria were isolated from Siberian sturgeon, European sea bass and giant river prawn. The capability of selected isolates to degrade PHB was confirmed in at least two of three setups: (1) growth in minimal medium containing PHB as the sole carbon (C) source, (2) production of clearing zones on minimal agar containing PHB as the sole C source and (3) degradation of PHB (as determined by HPLC analysis) in 10\% Luria-Bertani medium containing PHB. Challenge tests showed that the PHB-degrading activity of the selected isolates increased the survival of brine shrimp larvae challenged to a pathogenic Vibrio campbellii strain by a factor 2-3. Finally, one of the PHB-degrading isolates from sturgeon showed a double biocontrol effect because it was also able to inactivate acylhomoserine lactones, a type of quorum-sensing molecule that regulates the virulence of different pathogenic bacteria. Thus, the combined supplementation of a PHB-degrading bacterium and PHB as a synbioticum provides perspectives for improving the gastrointestinal health of aquatic animals.},
  author       = {Liu, Yiying and De Schryver, Peter and Van Delsen, Bart and Maignien, Lo{\"i}s and Boon, Nico and Sorgeloos, Patrick and Verstraete, Willy and Bossier, Peter and Defoirdt, Tom},
  issn         = {0168-6496},
  journal      = {FEMS MICROBIOLOGY ECOLOGY},
  keyword      = {PHB,polyhydroxyalkanoate,depolymerization,brine shrimp,probiotic,aquaculture,HYDROXYBUTYRATE-ACCUMULATING BACTERIA,GRADIENT GEL-ELECTROPHORESIS,QUORUM-SENSING SIGNALS,ARTEMIA-FRANCISCANA,MICROBIAL-DEGRADATION,AQUACULTURE,ANTIBIOTICS,INFECTIONS,CAMPBELLII,HARVEYI},
  language     = {eng},
  number       = {1},
  pages        = {196--204},
  title        = {PHB-degrading bacteria isolated from the gastrointestinal tract of aquatic animals as protective actors against luminescent vibriosis},
  url          = {http://dx.doi.org/10.1111/j.1574-6941.2010.00926.x},
  volume       = {74},
  year         = {2010},
}
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