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The small heat-shock protein HSP27 shows decreased expression in OA-chondrocytes and mediates IL-6 secretion in human articular chondrocytes

Stijn Lambrecht (UGent) , Karl Almqvist (UGent) , Peter Verdonk (UGent) , August Verbruggen (UGent) , Dieter Deforce (UGent) and Dirk Elewaut (UGent)
(2010) OSTEOARTHRITIS AND CARTILAGE. 8(suppl. 2). p.S107-S107
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Abstract
Background: HSP27 (HSPb1) is one of the small heat-shock proteins (sHSP), a protein family characterized by a molecular weight below 30 kDa. Recently, we reported the differential expression of alphaBcrystallin, another sHSP (Lambrecht et al, Arthritis Rheum, 2009). Based on the functional and structural relationship between alphaBcrystallin and HSP27, we further performed differential expression analysis on HSP27 Objectives: We aimed to achieve further insights in the involvement of small heat-shock proteins, more specific HSP27, in the biology of chondrocytes. Methods: Western blot and real-time RT-PCR analysis were performed to determine the expression levels of HSP27 in healthy and OA chondrocytes cultured in alginate beads. RNA-interference mediated gene knock-down was used to explore the role of this small heat shock protein in IL-1b activated pathways by transfecting low concentrations of siRNA in cultured chondrocytes. Upon knock-down of HSP27, cells were stimulated by IL-1b and IL-6 concentrations were determined by ELISA. Results: Western blot of healthy and OA chondrocyte lysates showed a decreased abundance of HSP27 in OA. Moreover, real-time RT-PCR confirmed the differential expression at the mRNA-level between chondrocytes isolated from visually intact and visually damaged zones of OA cartilage. The pro-inflammatory cytokines IL-1beta and TNF-alpha, both down regulated HP27 expression. Transfection of low concentrations siRNA in cultured chondrocytes resulted in an efficient knock down of HSP27 gene expression. This decreased HSP27 expression results in a reduced secretion of IL-6, in response to IL-1b. Conclusion: This study adds to the evidence that small heat-shock proteins may be important mediators in chondrocyte biology during the development of OA. Our study showed the involvement of HSP27 in IL-1b induced IL-6 secretion in human articular chondrocytes.

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MLA
Lambrecht, Stijn, Karl Almqvist, Peter Verdonk, et al. “The Small Heat-shock Protein HSP27 Shows Decreased Expression in OA-chondrocytes and Mediates IL-6 Secretion in Human Articular Chondrocytes.” Osteoarthritis and Cartilage. Vol. 8. 2010. S107–S107. Print.
APA
Lambrecht, Stijn, Almqvist, K., Verdonk, P., Verbruggen, A., Deforce, D., & Elewaut, D. (2010). The small heat-shock protein HSP27 shows decreased expression in OA-chondrocytes and mediates IL-6 secretion in human articular chondrocytes. OSTEOARTHRITIS AND CARTILAGE (Vol. 8, pp. S107–S107). Presented at the 2010 World Congress on Osteoarthitis.
Chicago author-date
Lambrecht, Stijn, Karl Almqvist, Peter Verdonk, August Verbruggen, Dieter Deforce, and Dirk Elewaut. 2010. “The Small Heat-shock Protein HSP27 Shows Decreased Expression in OA-chondrocytes and Mediates IL-6 Secretion in Human Articular Chondrocytes.” In Osteoarthritis and Cartilage, 8:S107–S107.
Chicago author-date (all authors)
Lambrecht, Stijn, Karl Almqvist, Peter Verdonk, August Verbruggen, Dieter Deforce, and Dirk Elewaut. 2010. “The Small Heat-shock Protein HSP27 Shows Decreased Expression in OA-chondrocytes and Mediates IL-6 Secretion in Human Articular Chondrocytes.” In Osteoarthritis and Cartilage, 8:S107–S107.
Vancouver
1.
Lambrecht S, Almqvist K, Verdonk P, Verbruggen A, Deforce D, Elewaut D. The small heat-shock protein HSP27 shows decreased expression in OA-chondrocytes and mediates IL-6 secretion in human articular chondrocytes. OSTEOARTHRITIS AND CARTILAGE. 2010. p. S107–S107.
IEEE
[1]
S. Lambrecht, K. Almqvist, P. Verdonk, A. Verbruggen, D. Deforce, and D. Elewaut, “The small heat-shock protein HSP27 shows decreased expression in OA-chondrocytes and mediates IL-6 secretion in human articular chondrocytes,” in OSTEOARTHRITIS AND CARTILAGE, Brussels, Belgium, 2010, vol. 8, no. suppl. 2, pp. S107–S107.
@inproceedings{1048627,
  abstract     = {Background: HSP27 (HSPb1) is one of the small heat-shock proteins (sHSP), a protein family characterized by a molecular weight below 30 kDa. Recently, we reported the differential expression of alphaBcrystallin, another sHSP (Lambrecht et al, Arthritis Rheum, 2009). Based on the functional and structural relationship between alphaBcrystallin and HSP27, we further performed differential expression analysis on HSP27
Objectives: We aimed to achieve further insights in the involvement of small heat-shock proteins, more specific HSP27,  in the biology of chondrocytes.
Methods: Western blot and real-time RT-PCR analysis were performed  to determine the expression levels of HSP27 in healthy and OA chondrocytes cultured in alginate beads. RNA-interference mediated gene knock-down was used to explore the role of this small heat shock protein in IL-1b activated pathways by transfecting low concentrations of siRNA in cultured chondrocytes. Upon knock-down of HSP27, cells were stimulated by IL-1b and IL-6 concentrations were determined by ELISA.
Results: Western blot of  healthy and OA chondrocyte lysates showed a decreased abundance of HSP27 in OA. Moreover, real-time RT-PCR confirmed the differential expression at the mRNA-level between chondrocytes isolated from visually intact and visually damaged zones of OA cartilage. The pro-inflammatory cytokines IL-1beta and TNF-alpha, both down regulated HP27 expression. Transfection of low concentrations siRNA in cultured chondrocytes resulted in an efficient knock down of HSP27 gene expression. This decreased HSP27 expression results in a reduced secretion of IL-6, in response to IL-1b.
Conclusion: This study adds to the evidence that small heat-shock proteins may be important mediators in chondrocyte biology during the development of OA. Our study showed the involvement of HSP27 in IL-1b  induced IL-6 secretion  in human articular chondrocytes.},
  articleno    = {abstract no. 231},
  author       = {Lambrecht, Stijn and Almqvist, Karl and Verdonk, Peter and Verbruggen, August and Deforce, Dieter and Elewaut, Dirk},
  booktitle    = {OSTEOARTHRITIS AND CARTILAGE},
  issn         = {1063-4584},
  language     = {eng},
  location     = {Brussels, Belgium},
  number       = {suppl. 2},
  pages        = {abstract no. 231:S107--abstract no. 231:S107},
  title        = {The small heat-shock protein HSP27 shows decreased expression in OA-chondrocytes and mediates IL-6 secretion in human articular chondrocytes},
  url          = {http://dx.doi.org/10.1016/S1063-4584(10)60258-0},
  volume       = {8},
  year         = {2010},
}

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