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Computational efficient segmentation of cell nuclei in 2D and 3D fluorescent micrographs

Jonas De Vylder UGent and Wilfried Philips UGent (2011) Proceedings of SPIE, the International Society for Optical Engineering. 7902.
abstract
This paper proposes a new segmentation technique developed for the segmentation of cell nuclei in both 2D and 3D fluorescent micrographs. The proposed method can deal with both blurred edges as with touching nuclei. Using a dual scan line algorithm its both memory as computational efficient, making it interesting for the analysis of images coming from high throughput systems or the analysis of 3D microscopic images. Experiments show good results, i.e. recall of over 0.98.
Please use this url to cite or link to this publication:
author
organization
year
type
conference
publication status
published
subject
in
Proceedings of SPIE, the International Society for Optical Engineering
Proc. SPIE Int. Soc. Opt. Eng.
editor
Daniel L Farkas, Dan V Nicolau and Robert C Leif
volume
7902
issue title
Imaging, manipulation, and analysis of biomolecules, cells, and tissues IX
article_number
79021P
pages
7 pages
publisher
SPIE, the International Society for Optical Engineering
place of publication
Bellingham, WA, USA
conference name
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX (2011 SPIE Photonics West)
conference location
San Francisco, CA, USA
conference start
2011-01-22
conference end
2011-01-25
Web of Science type
Proceedings Paper
Web of Science id
000297677500040
ISSN
0277-786X
1605-7422
ISBN
9780819484390
DOI
10.1117/12.872725
language
English
UGent publication?
yes
classification
P1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1048620
handle
http://hdl.handle.net/1854/LU-1048620
date created
2010-10-04 09:44:16
date last changed
2012-08-08 11:13:39
@inproceedings{1048620,
  abstract     = {This paper proposes a new segmentation technique developed for the segmentation of cell nuclei in both 2D and 3D fluorescent micrographs. The proposed method can deal with both blurred edges as with touching nuclei. Using a dual scan line algorithm its both memory as computational efficient, making it interesting for the analysis of images coming from high throughput systems or the analysis of 3D microscopic images. Experiments show good results, i.e. recall of over 0.98.},
  articleno    = {79021P},
  author       = {De Vylder, Jonas and Philips, Wilfried},
  booktitle    = {Proceedings of SPIE, the International Society for Optical Engineering},
  editor       = {Farkas, Daniel L and Nicolau, Dan V and Leif, Robert C},
  isbn         = {9780819484390},
  issn         = {0277-786X},
  language     = {eng},
  location     = {San Francisco, CA, USA},
  pages        = {7},
  publisher    = {SPIE, the International Society for Optical Engineering},
  title        = {Computational efficient segmentation of cell nuclei in 2D and 3D fluorescent micrographs},
  url          = {http://dx.doi.org/10.1117/12.872725},
  volume       = {7902},
  year         = {2011},
}

Chicago
De Vylder, Jonas, and Wilfried Philips. 2011. “Computational Efficient Segmentation of Cell Nuclei in 2D and 3D Fluorescent Micrographs.” In Proceedings of SPIE, the International Society for Optical Engineering, ed. Daniel L Farkas, Dan V Nicolau, and Robert C Leif. Vol. 7902. Bellingham, WA, USA: SPIE, the International Society for Optical Engineering.
APA
De Vylder, J., & Philips, W. (2011). Computational efficient segmentation of cell nuclei in 2D and 3D fluorescent micrographs. In D. L. Farkas, D. V. Nicolau, & R. C. Leif (Eds.), Proceedings of SPIE, the International Society for Optical Engineering (Vol. 7902). Presented at the Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX (2011 SPIE Photonics West), Bellingham, WA, USA: SPIE, the International Society for Optical Engineering.
Vancouver
1.
De Vylder J, Philips W. Computational efficient segmentation of cell nuclei in 2D and 3D fluorescent micrographs. In: Farkas DL, Nicolau DV, Leif RC, editors. Proceedings of SPIE, the International Society for Optical Engineering. Bellingham, WA, USA: SPIE, the International Society for Optical Engineering; 2011.
MLA
De Vylder, Jonas, and Wilfried Philips. “Computational Efficient Segmentation of Cell Nuclei in 2D and 3D Fluorescent Micrographs.” Proceedings of SPIE, the International Society for Optical Engineering. Ed. Daniel L Farkas, Dan V Nicolau, & Robert C Leif. Vol. 7902. Bellingham, WA, USA: SPIE, the International Society for Optical Engineering, 2011. Print.