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Short-term exposure to hydrogen peroxide during oocyte maturation improves bovine embryo development

Leen Vandaele UGent, M Thys, Jo Bijttebier UGent, A Vanlangendonckt, I Donnay, Dominiek Maes UGent, Evelyne Meyer UGent and Ann Van Soom UGent (2010) REPRODUCTION. 139(3). p.505-511
abstract
Recent studies have shown that short-term exposure of oocytes to a stressor such as hydrostatic pressure or osmotic stress might induce stress tolerance in embryos. The aim of the present study was to investigate the consequences of short-term hydrogen peroxide (H2O2) exposure to bovine in vitro matured cumulus-oocyte complexes (COCs) on subsequent preimplantation embryo development and apoptosis. in the first experiment, mature COCs were incubated in H2O2 at concentrations ranging between 0.01 and 100 mu mol/l, and subsequently fertilized and cultured. Oocyte incubation with 50-100 mu mol/l of H2O2 resulted in a significantly higher blastocyst yield (47.3%) in comparison with control medium (31.8%), while apoptotic cell ratio was inversely related with H2O2 concentration. In the second experiment, we showed that the stress tolerance after H2O2 exposure was not mediated by increased glutathione content in treated oocytes nor by enhanced fertilization or penetration. Further research should concentrate on the potential role of players that have been associated with stress tolerance in somatic cell lines. Reproduction (2010) 139 505-511
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
HIGH HYDROSTATIC-PRESSURE, IN-VITRO MATURATION, GROWTH-DIFFERENTIATION FACTOR-9, HYPOXIA-INDUCIBLE FACTOR-1, PROGRAMMED CELL-DEATH, OXIDATIVE STRESS, PREIMPLANTATION EMBRYO, SUPEROXIDE-DISMUTASE, PORCINE OOCYTES, GENE-EXPRESSION
journal title
REPRODUCTION
Reproduction
volume
139
issue
3
pages
505 - 511
Web of Science type
Article
Web of Science id
000275144500003
JCR category
REPRODUCTIVE BIOLOGY
JCR impact factor
3.049 (2010)
JCR rank
8/26 (2010)
JCR quartile
2 (2010)
ISSN
1470-1626
DOI
10.1530/REP-09-0430
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1048354
handle
http://hdl.handle.net/1854/LU-1048354
date created
2010-10-01 14:24:18
date last changed
2010-10-01 16:32:52
@article{1048354,
  abstract     = {Recent studies have shown that short-term exposure of oocytes to a stressor such as hydrostatic pressure or osmotic stress might induce stress tolerance in embryos. The aim of the present study was to investigate the consequences of short-term hydrogen peroxide (H2O2) exposure to bovine in vitro matured cumulus-oocyte complexes (COCs) on subsequent preimplantation embryo development and apoptosis. in the first experiment, mature COCs were incubated in H2O2 at concentrations ranging between 0.01 and 100 mu mol/l, and subsequently fertilized and cultured. Oocyte incubation with 50-100 mu mol/l of H2O2 resulted in a significantly higher blastocyst yield (47.3\%) in comparison with control medium (31.8\%), while apoptotic cell ratio was inversely related with H2O2 concentration. In the second experiment, we showed that the stress tolerance after H2O2 exposure was not mediated by increased glutathione content in treated oocytes nor by enhanced fertilization or penetration. Further research should concentrate on the potential role of players that have been associated with stress tolerance in somatic cell lines. Reproduction (2010) 139 505-511},
  author       = {Vandaele, Leen and Thys, M and Bijttebier, Jo and Vanlangendonckt, A and Donnay, I and Maes, Dominiek and Meyer, Evelyne and Van Soom, Ann},
  issn         = {1470-1626},
  journal      = {REPRODUCTION},
  keyword      = {HIGH HYDROSTATIC-PRESSURE,IN-VITRO MATURATION,GROWTH-DIFFERENTIATION FACTOR-9,HYPOXIA-INDUCIBLE FACTOR-1,PROGRAMMED CELL-DEATH,OXIDATIVE STRESS,PREIMPLANTATION EMBRYO,SUPEROXIDE-DISMUTASE,PORCINE OOCYTES,GENE-EXPRESSION},
  language     = {eng},
  number       = {3},
  pages        = {505--511},
  title        = {Short-term exposure to hydrogen peroxide during oocyte maturation improves bovine embryo development},
  url          = {http://dx.doi.org/10.1530/REP-09-0430},
  volume       = {139},
  year         = {2010},
}

Chicago
Vandaele, Leen, M Thys, Jo Bijttebier, A Vanlangendonckt, I Donnay, Dominiek Maes, Evelyne Meyer, and Ann Van Soom. 2010. “Short-term Exposure to Hydrogen Peroxide During Oocyte Maturation Improves Bovine Embryo Development.” Reproduction 139 (3): 505–511.
APA
Vandaele, L., Thys, M., Bijttebier, J., Vanlangendonckt, A., Donnay, I., Maes, D., Meyer, E., et al. (2010). Short-term exposure to hydrogen peroxide during oocyte maturation improves bovine embryo development. REPRODUCTION, 139(3), 505–511.
Vancouver
1.
Vandaele L, Thys M, Bijttebier J, Vanlangendonckt A, Donnay I, Maes D, et al. Short-term exposure to hydrogen peroxide during oocyte maturation improves bovine embryo development. REPRODUCTION. 2010;139(3):505–11.
MLA
Vandaele, Leen, M Thys, Jo Bijttebier, et al. “Short-term Exposure to Hydrogen Peroxide During Oocyte Maturation Improves Bovine Embryo Development.” REPRODUCTION 139.3 (2010): 505–511. Print.