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The effect of nutritionally induced hyperlipidaemia on in vitro bovine embryo quality

JMLR Leroy, V Van Hoeck, M Clemente, D Rizos, A Gutierrez-Adan, Ann Van Soom UGent, M Uytterhoeven and PEJ Bols (2010) HUMAN REPRODUCTION. 25(3). p.768-778
abstract
Obesity is associated with female reproductive abnormalities. Hyperlipidaemia might alter the embryonic micro-environment and potentially result in reduced fertility. We aimed to induce hyperlipidaemia nutritionally and investigate the consequences of hyperlipidaemic culture conditions on bovine in vitro embryo development, embryo quality and gene expression patterns. Bovine zygotes (n = 1545) were cultured in synthetic oviductal fluid medium supplemented with serum from heifers (n = 3), each fed three successive dietary treatments: (i) control serum, following a hay-based diet, (ii) hyperlipidaemic serum, following a carbohydrate and protected palm-oil-rich diet (FatCh) or (iii) hyperlipidaemic serum, following a protected palm-oil-rich diet (Fat). Blastocysts were evaluated for development, cell count, picnotic and mitotic indexes and cryotolerance. Selected mRNA transcripts were measured by quantitative RT-PCR. FatCh and Fat diets approximately doubled the total cholesterol concentrations, compared with controls (167.1 +/- 11.9, 150.0 +/- 12.8 versus 83.4 +/- 13.7 mg/dl, respectively, P < 0.05), and fatty acid concentrations (8146.60 +/- 214.61, 6935.56 +/- 1081.04 versus 3944.0 +/- 425.07 mu mol/l, respectively, P < 0.05). Supplementation of culture media with FatCh and Fat serum significantly reduced blastocyst rates, compared with controls (27.8, 23.4% versus 36.2%, respectively, P < 0.01), total cell number (103.3 +/- 30.1, 95.6 +/- 28.2 versus 146.9 +/- 34.2, respectively, P < 0.01), mitotic index (1.3 +/- 1.1, 1.7 +/- 2.4 versus 3.6 +/- 2.2%, respectively, P < 0.01) and hatching rates after vitrification (20.4, 13.8 versus 35.7%, respectively, P = 0.03). Embryos in FatCh and Fat groups exhibited significantly higher mRNA levels for genes related to apoptosis and metabolism, compared with controls. This combined in vivo and in vitro model indicates that the exposure of preimplantation embryos to hyperlipidaemic conditions may result in reduced embryo quality and developmental potential, possibly resulting in poorer fertility.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
journal title
HUMAN REPRODUCTION
Hum. Reprod.
volume
25
issue
3
pages
768 - 778
Web of Science type
Article
Web of Science id
000274490700029
JCR category
OBSTETRICS & GYNECOLOGY
JCR impact factor
4.357 (2010)
JCR rank
3/75 (2010)
JCR quartile
1 (2010)
ISSN
0268-1161
DOI
10.1093/humrep/dep420
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1048321
handle
http://hdl.handle.net/1854/LU-1048321
date created
2010-10-01 14:04:39
date last changed
2016-12-19 15:44:33
@article{1048321,
  abstract     = {Obesity is associated with female reproductive abnormalities. Hyperlipidaemia might alter the embryonic micro-environment and potentially result in reduced fertility. We aimed to induce hyperlipidaemia nutritionally and investigate the consequences of hyperlipidaemic culture conditions on bovine in vitro embryo development, embryo quality and gene expression patterns.
Bovine zygotes (n = 1545) were cultured in synthetic oviductal fluid medium supplemented with serum from heifers (n = 3), each fed three successive dietary treatments: (i) control serum, following a hay-based diet, (ii) hyperlipidaemic serum, following a carbohydrate and protected palm-oil-rich diet (FatCh) or (iii) hyperlipidaemic serum, following a protected palm-oil-rich diet (Fat). Blastocysts were evaluated for development, cell count, picnotic and mitotic indexes and cryotolerance. Selected mRNA transcripts were measured by quantitative RT-PCR.
FatCh and Fat diets approximately doubled the total cholesterol concentrations, compared with controls (167.1 +/- 11.9, 150.0 +/- 12.8 versus 83.4 +/- 13.7 mg/dl, respectively, P {\textlangle} 0.05), and fatty acid concentrations (8146.60 +/- 214.61, 6935.56 +/- 1081.04 versus 3944.0 +/- 425.07 mu mol/l, respectively, P {\textlangle} 0.05). Supplementation of culture media with FatCh and Fat serum significantly reduced blastocyst rates, compared with controls (27.8, 23.4\% versus 36.2\%, respectively, P {\textlangle} 0.01), total cell number (103.3 +/- 30.1, 95.6 +/- 28.2 versus 146.9 +/- 34.2, respectively, P {\textlangle} 0.01), mitotic index (1.3 +/- 1.1, 1.7 +/- 2.4 versus 3.6 +/- 2.2\%, respectively, P {\textlangle} 0.01) and hatching rates after vitrification (20.4, 13.8 versus 35.7\%, respectively, P = 0.03). Embryos in FatCh and Fat groups exhibited significantly higher mRNA levels for genes related to apoptosis and metabolism, compared with controls.
This combined in vivo and in vitro model indicates that the exposure of preimplantation embryos to hyperlipidaemic conditions may result in reduced embryo quality and developmental potential, possibly resulting in poorer fertility.},
  author       = {Leroy, JMLR and Van Hoeck, V and Clemente, M and Rizos, D and Gutierrez-Adan, A and Van Soom, Ann and Uytterhoeven, M and Bols, PEJ},
  issn         = {0268-1161},
  journal      = {HUMAN REPRODUCTION},
  language     = {eng},
  number       = {3},
  pages        = {768--778},
  title        = {The effect of nutritionally induced hyperlipidaemia on in vitro bovine embryo quality},
  url          = {http://dx.doi.org/10.1093/humrep/dep420},
  volume       = {25},
  year         = {2010},
}

Chicago
Leroy, JMLR, V Van Hoeck, M Clemente, D Rizos, A Gutierrez-Adan, Ann Van Soom, M Uytterhoeven, and PEJ Bols. 2010. “The Effect of Nutritionally Induced Hyperlipidaemia on in Vitro Bovine Embryo Quality.” Human Reproduction 25 (3): 768–778.
APA
Leroy, JMLR, Van Hoeck, V., Clemente, M., Rizos, D., Gutierrez-Adan, A., Van Soom, A., Uytterhoeven, M., et al. (2010). The effect of nutritionally induced hyperlipidaemia on in vitro bovine embryo quality. HUMAN REPRODUCTION, 25(3), 768–778.
Vancouver
1.
Leroy J, Van Hoeck V, Clemente M, Rizos D, Gutierrez-Adan A, Van Soom A, et al. The effect of nutritionally induced hyperlipidaemia on in vitro bovine embryo quality. HUMAN REPRODUCTION. 2010;25(3):768–78.
MLA
Leroy, JMLR, V Van Hoeck, M Clemente, et al. “The Effect of Nutritionally Induced Hyperlipidaemia on in Vitro Bovine Embryo Quality.” HUMAN REPRODUCTION 25.3 (2010): 768–778. Print.