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Comparison of culture and qPCR for the detection of Pseudomonas aeruginosa in not chronically infected cystic fibrosis patients

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Abstract
Background: Pseudomonas aeruginosa is the major respiratory pathogen causing severe lung infections among CF patients, leading to high morbidity and mortality. Once infection is established, early antibiotic treatment is able to postpone the transition to chronic lung infection. In order to optimize the early detection, we compared the sensitivity of microbiological culture and quantitative PCR (qPCR) for the detection of P. aeruginosa in respiratory samples of not chronically infected CF patients. Results: In this national study, we followed CF patients during periods between 1 to 15 months. For a total of 852 samples, 729 (86%) remained P. aeruginosa negative by both culture and qPCR, whereas 89 samples (10%) were positive by both culture and qPCR. Twenty-six samples were negative by culture but positive by qPCR, and 10 samples were positive by culture but remained negative by qPCR. Five of the 26 patients with a culture negative, qPCR positive sample became later P. aeruginosa positive both by culture and qPCR. Conclusion: Based on the results of this study, it can be concluded that qPCR may have a predictive value for impending P. aeruginosa infection for only a limited number of patients.
Keywords
MELTING CURVE ANALYSIS, POLYMERASE-CHAIN-REACTION, REAL-TIME PCR, BURKHOLDERIA-CEPACIA, PULMONARY-FUNCTION, CLINICAL-SAMPLES, RISK-FACTORS, IDENTIFICATION, COLONIZATION, DIAGNOSIS

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Chicago
Deschaght, Pieter, Petra Schelstraete, Guido Lopes dos Santos Santiago, Leen Van Simaey, Filomeen Haerynck, Van daeleSabine, Elke De Wachter, et al. 2010. “Comparison of Culture and qPCR for the Detection of Pseudomonas Aeruginosa in Not Chronically Infected Cystic Fibrosis Patients.” Bmc Microbiology 10.
APA
Deschaght, P., Schelstraete, P., Lopes dos Santos Santiago, G., Van Simaey, L., Haerynck, F., Van daeleSabine, De Wachter, E., et al. (2010). Comparison of culture and qPCR for the detection of Pseudomonas aeruginosa in not chronically infected cystic fibrosis patients. BMC MICROBIOLOGY, 10.
Vancouver
1.
Deschaght P, Schelstraete P, Lopes dos Santos Santiago G, Van Simaey L, Haerynck F, Van daeleSabine, et al. Comparison of culture and qPCR for the detection of Pseudomonas aeruginosa in not chronically infected cystic fibrosis patients. BMC MICROBIOLOGY. 2010;10.
MLA
Deschaght, Pieter, Petra Schelstraete, Guido Lopes dos Santos Santiago, et al. “Comparison of Culture and qPCR for the Detection of Pseudomonas Aeruginosa in Not Chronically Infected Cystic Fibrosis Patients.” BMC MICROBIOLOGY 10 (2010): n. pag. Print.
@article{1047010,
  abstract     = {Background: Pseudomonas aeruginosa is the major respiratory pathogen causing severe lung infections among CF patients, leading to high morbidity and mortality. Once infection is established, early antibiotic treatment is able to postpone the transition to chronic lung infection. In order to optimize the early detection, we compared the sensitivity of microbiological culture and quantitative PCR (qPCR) for the detection of P. aeruginosa in respiratory samples of not chronically infected CF patients.
Results: In this national study, we followed CF patients during periods between 1 to 15 months. For a total of 852 samples, 729 (86\%) remained P. aeruginosa negative by both culture and qPCR, whereas 89 samples (10\%) were positive by both culture and qPCR. Twenty-six samples were negative by culture but positive by qPCR, and 10 samples were positive by culture but remained negative by qPCR. Five of the 26 patients with a culture negative, qPCR positive sample became later P. aeruginosa positive both by culture and qPCR.
Conclusion: Based on the results of this study, it can be concluded that qPCR may have a predictive value for impending P. aeruginosa infection for only a limited number of patients.},
  articleno    = {245},
  author       = {Deschaght, Pieter and Schelstraete, Petra and Lopes dos Santos Santiago, Guido and Van Simaey, Leen and Haerynck, Filomeen and Van daele, Sabine and  De Wachter, Elke and Malfroot, Anne  and Lebecque, Patrick  and Knoop, Christiane  and Casimir, Georges  and Boboli, Hedwige  and Pierart, Fr{\'e}d{\'e}ric  and Desager, Kristine  and Vaneechoutte, Mario and De Baets, Frans},
  issn         = {1471-2180},
  journal      = {BMC MICROBIOLOGY},
  keyword      = {MELTING CURVE ANALYSIS,POLYMERASE-CHAIN-REACTION,REAL-TIME PCR,BURKHOLDERIA-CEPACIA,PULMONARY-FUNCTION,CLINICAL-SAMPLES,RISK-FACTORS,IDENTIFICATION,COLONIZATION,DIAGNOSIS},
  language     = {eng},
  pages        = {6},
  title        = {Comparison of culture and qPCR for the detection of Pseudomonas aeruginosa in not chronically infected cystic fibrosis patients},
  url          = {http://dx.doi.org/10.1186/1471-2180-10-245},
  volume       = {10},
  year         = {2010},
}

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