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Flexible nanosomes (SECosomes) enable efficient siRNA delivery in cultured primary skin cells and in the viable epidermis of ex vivo human skin

(2010) ADVANCED FUNCTIONAL MATERIALS. 20(23). p.4077-4090
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Abstract
The extent to which nanoscale-engineered systems cross intact human skin and can exert pharmacological effects in viable epidermis is controversial. This research seeks to develop a new lipid-based nanosome that enables the effective delivery of siRNA into human skin. The major finding is that an ultrafl exible siRNA-containing nanosome—prepared using DOTAP, cholesterol, sodium cholate, and 30% ethanol penetrates into the epidermis of freshly excised intact human skin and is able to enter into the keratinocytes. The nanosomes, called surfactant-ethanolcholesterolosomes (SECosomes), show excellent size, surface charge, morphology, deformability, transfection effi ciency, stability, and skin penetration capacity after complexation with siRNA. Importantly, these nanosomes have ideal characteristics for siRNA encapsulation, in that the siRNA is stable for at least 4 weeks, they enable highly efficient transfection of in vitro cultured cells, and are shown to transport siRNA delivery through intact human skin where changes in the keratinocyte cell state are demonstrated. It is concluded that increasing flexibility in nanosomes greatly enhances their ability to cross the intact human epidermal membrane and to unload their payload into targeted epidermal cells.
Keywords
PICOSECOND TIME RESOLUTION, ULTRADEFORMABLE DRUG CARRIERS, IN-VIVO, LIPID VESICLES, TRANSDERMAL DELIVERY, TOPICAL DELIVERY, MULTIPHOTON MICROSCOPY, STANDARD LIPOSOMES, SPATIAL-RESOLUTION, CATIONIC LIPOSOME

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Citation

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Chicago
Geusens, Barbara, Mireille Van Gele, Sien Braat, Stefaan De Smedt, Marc CA Stuart, Tarl W Prow, Washington Sanchez, Michael S Roberts, Niek Sanders, and Jo Lambert. 2010. “Flexible Nanosomes (SECosomes) Enable Efficient siRNA Delivery in Cultured Primary Skin Cells and in the Viable Epidermis of Ex Vivo Human Skin.” Advanced Functional Materials 20 (23): 4077–4090.
APA
Geusens, B., Van Gele, M., Braat, S., De Smedt, S., Stuart, M. C., Prow, T. W., Sanchez, W., et al. (2010). Flexible nanosomes (SECosomes) enable efficient siRNA delivery in cultured primary skin cells and in the viable epidermis of ex vivo human skin. ADVANCED FUNCTIONAL MATERIALS, 20(23), 4077–4090.
Vancouver
1.
Geusens B, Van Gele M, Braat S, De Smedt S, Stuart MC, Prow TW, et al. Flexible nanosomes (SECosomes) enable efficient siRNA delivery in cultured primary skin cells and in the viable epidermis of ex vivo human skin. ADVANCED FUNCTIONAL MATERIALS. 2010;20(23):4077–90.
MLA
Geusens, Barbara, Mireille Van Gele, Sien Braat, et al. “Flexible Nanosomes (SECosomes) Enable Efficient siRNA Delivery in Cultured Primary Skin Cells and in the Viable Epidermis of Ex Vivo Human Skin.” ADVANCED FUNCTIONAL MATERIALS 20.23 (2010): 4077–4090. Print.
@article{1046715,
  abstract     = {The extent to which nanoscale-engineered systems cross intact human skin and can exert pharmacological effects in viable epidermis is controversial. This research seeks to develop a new lipid-based nanosome that enables the effective delivery of siRNA into human skin. The major finding is that an ultrafl exible siRNA-containing nanosome---prepared using DOTAP, cholesterol, sodium cholate, and 30\% ethanol penetrates into the epidermis of freshly excised intact human skin and is able to enter into the keratinocytes. The nanosomes, called surfactant-ethanolcholesterolosomes (SECosomes), show excellent size, surface charge, morphology, deformability, transfection effi ciency, stability, and skin penetration capacity after complexation with siRNA. Importantly, these nanosomes have ideal characteristics for siRNA encapsulation, in that the siRNA is stable for at least 4 weeks, they enable highly efficient transfection of in vitro cultured cells, and are shown to transport siRNA delivery through intact human skin where changes in the keratinocyte cell state are demonstrated. It is concluded that increasing flexibility in nanosomes greatly enhances their ability to cross the intact human epidermal membrane and to unload their payload into targeted epidermal cells.},
  author       = {Geusens, Barbara and Van Gele, Mireille and Braat, Sien and De Smedt, Stefaan and Stuart, Marc CA  and Prow, Tarl W and Sanchez, Washington and Roberts, Michael S and Sanders, Niek and Lambert, Jo},
  issn         = {1616-301X},
  journal      = {ADVANCED FUNCTIONAL MATERIALS},
  keyword      = {PICOSECOND TIME RESOLUTION,ULTRADEFORMABLE DRUG CARRIERS,IN-VIVO,LIPID VESICLES,TRANSDERMAL DELIVERY,TOPICAL DELIVERY,MULTIPHOTON MICROSCOPY,STANDARD LIPOSOMES,SPATIAL-RESOLUTION,CATIONIC LIPOSOME},
  language     = {eng},
  number       = {23},
  pages        = {4077--4090},
  title        = {Flexible nanosomes (SECosomes) enable efficient siRNA delivery in cultured primary skin cells and in the viable epidermis of ex vivo human skin},
  url          = {http://dx.doi.org/10.1002/adfm.201000484},
  volume       = {20},
  year         = {2010},
}

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