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Affinity study of novel gelatin cell carriers for fibronectin

Sandra Van Vlierberghe (UGent) , Els Vanderleyden (UGent) , Peter Dubruel (UGent) , Filip De Vos (UGent) and Etienne Schacht (UGent)
(2009) MACROMOLECULAR BIOSCIENCE. 9(11). p.1105-1115
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Abstract
In the present work, the gelatin/fibronectin affinity was evaluated using SPR, QCM and radiolabelling. The results indicate that type A gelatin films possess a higher affinity for Fn compared to type B gelatin. This is due to a combined hydrophobic and electrostatic interaction between gelatin type A and Fn. In a second part, the affinity of Fn for porous gelatin scaffolds was evaluated. The scaffolds were prepared by a cryogenic treatment and subsequent freeze-drying yielding type I and type II scaffolds which possess different pore geometries/sizes. The results indicate that the Fn density on the scaffolds can be fine-tuned by varying the Fn concentration, the gelatin type (A vs. 13), the pore size/geometry (type I vs. type II scaffolds).
Keywords
CONTROLLED DELIVERY, PROTEIN ADSORPTION, CHEMICAL-MODIFICATION, HUMAN-PLASMA FIBRONECTIN, surface plasmon resonance, radiolabeling, quartz crystal microbalance, hydrogels, proteins, TITANIUM SURFACES, IN-VITRO, BINDING, ATTACHMENT, HYDROGELS, MIGRATION

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Please use this url to cite or link to this publication:

Chicago
Van Vlierberghe, Sandra, Els Vanderleyden, Peter Dubruel, Filip De Vos, and Etienne Schacht. 2009. “Affinity Study of Novel Gelatin Cell Carriers for Fibronectin.” Macromolecular Bioscience 9 (11): 1105–1115.
APA
Van Vlierberghe, Sandra, Vanderleyden, E., Dubruel, P., De Vos, F., & Schacht, E. (2009). Affinity study of novel gelatin cell carriers for fibronectin. MACROMOLECULAR BIOSCIENCE, 9(11), 1105–1115.
Vancouver
1.
Van Vlierberghe S, Vanderleyden E, Dubruel P, De Vos F, Schacht E. Affinity study of novel gelatin cell carriers for fibronectin. MACROMOLECULAR BIOSCIENCE. 2009;9(11):1105–15.
MLA
Van Vlierberghe, Sandra, Els Vanderleyden, Peter Dubruel, et al. “Affinity Study of Novel Gelatin Cell Carriers for Fibronectin.” MACROMOLECULAR BIOSCIENCE 9.11 (2009): 1105–1115. Print.
@article{1046358,
  abstract     = {In the present work, the gelatin/fibronectin affinity was evaluated using SPR, QCM and radiolabelling. The results indicate that type A gelatin films possess a higher affinity for Fn compared to type B gelatin. This is due to a combined hydrophobic and electrostatic interaction between gelatin type A and Fn. In a second part, the affinity of Fn for porous gelatin scaffolds was evaluated. The scaffolds were prepared by a cryogenic treatment and subsequent freeze-drying yielding type I and type II scaffolds which possess different pore geometries/sizes. The results indicate that the Fn density on the scaffolds can be fine-tuned by varying the Fn concentration, the gelatin type (A vs. 13), the pore size/geometry (type I vs. type II scaffolds).},
  author       = {Van Vlierberghe, Sandra and Vanderleyden, Els and Dubruel, Peter and De Vos, Filip and Schacht, Etienne},
  issn         = {1616-5187},
  journal      = {MACROMOLECULAR BIOSCIENCE},
  keywords     = {CONTROLLED DELIVERY,PROTEIN ADSORPTION,CHEMICAL-MODIFICATION,HUMAN-PLASMA FIBRONECTIN,surface plasmon resonance,radiolabeling,quartz crystal microbalance,hydrogels,proteins,TITANIUM SURFACES,IN-VITRO,BINDING,ATTACHMENT,HYDROGELS,MIGRATION},
  language     = {eng},
  number       = {11},
  pages        = {1105--1115},
  title        = {Affinity study of novel gelatin cell carriers for fibronectin},
  url          = {http://dx.doi.org/10.1002/mabi.200900043},
  volume       = {9},
  year         = {2009},
}

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