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Immunolocalization of proteins in plants

Michael Sauer and Jiri Friml UGent (2010) Plant developmental biology : methods and protocols. In Methods in Molecular Biology 655. p.253-263
abstract
Rapid advances in the field of plant biology, especially in plant cell biology, have created the need for methods that allow the localization of proteins in situ at subcellular resolution. Although in many cases recombinant proteins with fluorescent proteins can fulfill this task, antibody-based immunological detection of proteins is a complementary technique, which avoids the risk of inducing side effects by a fusion protein, such as misexpression, mistargeting, altered stability, or toxicity. Moreover, recombinant protein techniques are applicable only to a rather limited set of model plants. The immunolocalization protocols presented here can be used to display protein localization patterns in different tissues of various plant species. This chapter describes a whole mount immunolocalization protocol, which has been extensively used in Arabidopsis roots and some above-ground tissues, and that also works in other species. Additionally, for bulky or hard tissue types, a variation of this protocol for paraffin-embedded sections is given.
Please use this url to cite or link to this publication:
author
organization
year
type
bookChapter
publication status
published
subject
keyword
antibody-based detection, Immunocytochemistry, immunolocalization, fluorescence microscopy
book title
Plant developmental biology : methods and protocols
editor
Lars Hennig and Claudia Köhler
series title
Methods in Molecular Biology
volume
655
pages
253 - 263
publisher
Springer
place of publication
New York, NY, USA
ISBN
9781607617648
DOI
10.1007/978-1-60761-765-5_17
language
English
UGent publication?
yes
classification
B2
copyright statement
I have transferred the copyright for this publication to the publisher
id
1040417
handle
http://hdl.handle.net/1854/LU-1040417
date created
2010-09-09 17:23:19
date last changed
2012-09-19 14:04:00
@incollection{1040417,
  abstract     = {Rapid advances in the field of plant biology, especially in plant cell biology, have created the need for methods that allow the localization of proteins in situ at subcellular resolution. Although in many cases recombinant proteins with fluorescent proteins can fulfill this task, antibody-based immunological detection of proteins is a complementary technique, which avoids the risk of inducing side effects by a fusion protein, such as misexpression, mistargeting, altered stability, or toxicity. Moreover, recombinant protein techniques are applicable only to a rather limited set of model plants. The immunolocalization protocols presented here can be used to display protein localization patterns in different tissues of various plant species. This chapter describes a whole mount immunolocalization protocol, which has been extensively used in Arabidopsis roots and some above-ground tissues, and that also works in other species. Additionally, for bulky or hard tissue types, a variation of this protocol for paraffin-embedded sections is given.},
  author       = {Sauer, Michael and Friml, Jiri},
  booktitle    = {Plant developmental biology : methods and protocols},
  editor       = {Hennig, Lars and K{\"o}hler, Claudia},
  isbn         = {9781607617648},
  keyword      = {antibody-based detection,Immunocytochemistry,immunolocalization,fluorescence microscopy},
  language     = {eng},
  pages        = {253--263},
  publisher    = {Springer},
  series       = {Methods in Molecular Biology},
  title        = {Immunolocalization of proteins in plants},
  url          = {http://dx.doi.org/10.1007/978-1-60761-765-5\_17},
  volume       = {655},
  year         = {2010},
}

Chicago
Sauer, Michael, and Jiri Friml. 2010. “Immunolocalization of Proteins in Plants.” In Plant Developmental Biology : Methods and Protocols, ed. Lars Hennig and Claudia Köhler, 655:253–263. New York, NY, USA: Springer.
APA
Sauer, M., & Friml, J. (2010). Immunolocalization of proteins in plants. In L. Hennig & C. Köhler (Eds.), Plant developmental biology : methods and protocols (Vol. 655, pp. 253–263). New York, NY, USA: Springer.
Vancouver
1.
Sauer M, Friml J. Immunolocalization of proteins in plants. In: Hennig L, Köhler C, editors. Plant developmental biology : methods and protocols. New York, NY, USA: Springer; 2010. p. 253–63.
MLA
Sauer, Michael, and Jiri Friml. “Immunolocalization of Proteins in Plants.” Plant Developmental Biology : Methods and Protocols. Ed. Lars Hennig & Claudia Köhler. Vol. 655. New York, NY, USA: Springer, 2010. 253–263. Print.