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Kinematic analysis of cell division and expansion

Bart Rymen (UGent) , Frederik Coppens (UGent) , Stijn Dhondt (UGent) , Fabio Fiorani (UGent) and Gerrit Beemster (UGent)
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Abstract
Plant growth is readily analysed at the macroscopic level by measuring size and/or mass. Although it is commonly known that the rate of growth is determined by cell division and subsequent cell expansion, relatively few studies describing growth phenotypes include studies of the dynamics of these processes. Kinematic analyses provide a powerful and rigorous framework to perform such studies and have been adapted to the specific characteristics of various plant organs. Here we describe in detail how to perform these analyses in root tips and leaves of the model species Arabidopsis thaliana and in the leaves of the monocotyledonous crop species, Zea mays. These methods can be readily used and adapted to suit other species in most laboratories.
Keywords
leaf growth, Arabidopsis, epidermal cells, image analysis, kinematic analysis, Cell division, cell expansion, root growth, maize

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Citation

Please use this url to cite or link to this publication:

Chicago
Rymen, Bart, Frederik Coppens, Stijn Dhondt, Fabio Fiorani, and Gerrit Beemster. 2010. “Kinematic Analysis of Cell Division and Expansion.” In Plant Developmental Biology : Methods and Protocols, ed. Lars Hennig and Claudia Köhler, 655:203–227. New York, NY, USA: Springer.
APA
Rymen, B., Coppens, F., Dhondt, S., Fiorani, F., & Beemster, G. (2010). Kinematic analysis of cell division and expansion. In L. Hennig & C. Köhler (Eds.), Plant developmental biology : methods and protocols (Vol. 655, pp. 203–227). New York, NY, USA: Springer.
Vancouver
1.
Rymen B, Coppens F, Dhondt S, Fiorani F, Beemster G. Kinematic analysis of cell division and expansion. In: Hennig L, Köhler C, editors. Plant developmental biology : methods and protocols. New York, NY, USA: Springer; 2010. p. 203–27.
MLA
Rymen, Bart, Frederik Coppens, Stijn Dhondt, et al. “Kinematic Analysis of Cell Division and Expansion.” Plant Developmental Biology : Methods and Protocols. Ed. Lars Hennig & Claudia Köhler. Vol. 655. New York, NY, USA: Springer, 2010. 203–227. Print.
@incollection{1040408,
  abstract     = {Plant growth is readily analysed at the macroscopic level by measuring size and/or mass. Although it is commonly known that the rate of growth is determined by cell division and subsequent cell expansion, relatively few studies describing growth phenotypes include studies of the dynamics of these processes. Kinematic analyses provide a powerful and rigorous framework to perform such studies and have been adapted to the specific characteristics of various plant organs. Here we describe in detail how to perform these analyses in root tips and leaves of the model species Arabidopsis thaliana and in the leaves of the monocotyledonous crop species, Zea mays. These methods can be readily used and adapted to suit other species in most laboratories.},
  author       = {Rymen, Bart and Coppens, Frederik and Dhondt, Stijn and Fiorani, Fabio and Beemster, Gerrit},
  booktitle    = {Plant developmental biology : methods and protocols},
  editor       = {Hennig, Lars  and K{\"o}hler, Claudia},
  isbn         = {9781607617648},
  keyword      = {leaf growth,Arabidopsis,epidermal cells,image analysis,kinematic analysis,Cell division,cell expansion,root growth,maize},
  language     = {eng},
  pages        = {203--227},
  publisher    = {Springer},
  series       = {Methods in Molecular Biology},
  title        = {Kinematic analysis of cell division and expansion},
  url          = {http://dx.doi.org/10.1007/978-1-60761-765-5\_14},
  volume       = {655},
  year         = {2010},
}

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