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Rapid and direct quantification of viable Candida species in whole blood by use of immunomagnetic separation and solid-phase cytometry

Lies Vanhee UGent, Wouter Meersseman, Katrien Lagrou, Johan Maertens, Hans Nelis UGent and Tom Coenye UGent (2010) JOURNAL OF CLINICAL MICROBIOLOGY. 48(4). p.1126-1131
abstract
Candida species are a common source of nosocomial bloodstream infections in critically ill patients. The sensitivity of the traditional diagnostic procedure based on blood culture is variable, and it usually takes 2 to 4 days before growth of Candida species is detected. We developed a 4-h method for the quantification of Candida species in blood, combining immunomagnetic separation (IMS) with solid-phase cytometry (SPC) using viability labeling. Additionally, Candida albicans cells could be identified in real time by using fluorescent in situ hybridization. By analysis of spiked blood samples, our method was shown to be sensitive and specific, with a low detection limit (1 cell/ml of blood). In a proof-of-concept study, we applied the IMS/SPC method to 16 clinical samples and compared it to traditional blood culture. Our method proved more sensitive than culture (seven samples were positive with IMS/SPC but negative with blood culture), and identification results were in agreement. The IMS/SPC data also suggest that mixed infections might occur frequently, as C. albicans and at least one other Candida species were found in five samples. Additionally, in two cases, high numbers of cells (175 to 480 cells/ml of blood) were associated with an endovascular source of infection.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
DIAGNOSIS, WATER, IDENTIFICATION, BACTERIA, STREAM INFECTIONS, LIGHT-CYCLER SYSTEM, ENUMERATION, MICROSCOPY, CULTURES, FUNGI
journal title
JOURNAL OF CLINICAL MICROBIOLOGY
J. Clin. Microbiol.
volume
48
issue
4
pages
1126 - 1131
Web of Science type
Article
Web of Science id
000276153200015
JCR category
MICROBIOLOGY
JCR impact factor
4.22 (2010)
JCR rank
20/103 (2010)
JCR quartile
1 (2010)
ISSN
0095-1137
DOI
10.1128/JCM.00035-10
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1039007
handle
http://hdl.handle.net/1854/LU-1039007
date created
2010-09-09 10:11:27
date last changed
2016-12-19 15:46:28
@article{1039007,
  abstract     = {Candida species are a common source of nosocomial bloodstream infections in critically ill patients. The sensitivity of the traditional diagnostic procedure based on blood culture is variable, and it usually takes 2 to 4 days before growth of Candida species is detected. We developed a 4-h method for the quantification of Candida species in blood, combining immunomagnetic separation (IMS) with solid-phase cytometry (SPC) using viability labeling. Additionally, Candida albicans cells could be identified in real time by using fluorescent in situ hybridization. By analysis of spiked blood samples, our method was shown to be sensitive and specific, with a low detection limit (1 cell/ml of blood). In a proof-of-concept study, we applied the IMS/SPC method to 16 clinical samples and compared it to traditional blood culture. Our method proved more sensitive than culture (seven samples were positive with IMS/SPC but negative with blood culture), and identification results were in agreement. The IMS/SPC data also suggest that mixed infections might occur frequently, as C. albicans and at least one other Candida species were found in five samples. Additionally, in two cases, high numbers of cells (175 to 480 cells/ml of blood) were associated with an endovascular source of infection.},
  author       = {Vanhee, Lies and Meersseman, Wouter and Lagrou, Katrien and Maertens, Johan and Nelis, Hans and Coenye, Tom},
  issn         = {0095-1137},
  journal      = {JOURNAL OF CLINICAL MICROBIOLOGY},
  keyword      = {DIAGNOSIS,WATER,IDENTIFICATION,BACTERIA,STREAM INFECTIONS,LIGHT-CYCLER SYSTEM,ENUMERATION,MICROSCOPY,CULTURES,FUNGI},
  language     = {eng},
  number       = {4},
  pages        = {1126--1131},
  title        = {Rapid and direct quantification of viable Candida species in whole blood by use of immunomagnetic separation and solid-phase cytometry},
  url          = {http://dx.doi.org/10.1128/JCM.00035-10},
  volume       = {48},
  year         = {2010},
}

Chicago
Vanhee, Lies, Wouter Meersseman, Katrien Lagrou, Johan Maertens, Hans Nelis, and Tom Coenye. 2010. “Rapid and Direct Quantification of Viable Candida Species in Whole Blood by Use of Immunomagnetic Separation and Solid-phase Cytometry.” Journal of Clinical Microbiology 48 (4): 1126–1131.
APA
Vanhee, L., Meersseman, W., Lagrou, K., Maertens, J., Nelis, H., & Coenye, T. (2010). Rapid and direct quantification of viable Candida species in whole blood by use of immunomagnetic separation and solid-phase cytometry. JOURNAL OF CLINICAL MICROBIOLOGY, 48(4), 1126–1131.
Vancouver
1.
Vanhee L, Meersseman W, Lagrou K, Maertens J, Nelis H, Coenye T. Rapid and direct quantification of viable Candida species in whole blood by use of immunomagnetic separation and solid-phase cytometry. JOURNAL OF CLINICAL MICROBIOLOGY. 2010;48(4):1126–31.
MLA
Vanhee, Lies, Wouter Meersseman, Katrien Lagrou, et al. “Rapid and Direct Quantification of Viable Candida Species in Whole Blood by Use of Immunomagnetic Separation and Solid-phase Cytometry.” JOURNAL OF CLINICAL MICROBIOLOGY 48.4 (2010): 1126–1131. Print.