Rhodococcus fascians impacts plant development through the dynamic Fas-mediated production of a cytokinin mix
- Author
- Ine Pertry (UGent) , Katerina Václavíková, Markéta Gemrotová, Lukáš Spíchal, Petr Galuszka, Stephen Depuydt (UGent) , Wim Temmerman (UGent) , Elisabeth Stes (UGent) , Annick De Keyser (UGent) , Michael Riefler, Stefania Biondi, Ondrej Novak, Thomas Schmülling, Miroslav Strnad, Petr Tarkowski, Marcella Holsters (UGent) and Danny Vereecke (UGent)
- Organization
- Abstract
- The phytopathogenic actinomycete Rhodococcus fascians D188 relies mainly on the linear plasmid-encoded fas operon for its virulence. The bacteria secrete six cytokinin bases that synergistically redirect the developmental program of the plant to stimulate proliferation of young shoot tissue, thus establishing a leafy gall as a niche. A yeast-based cytokinin bioassay combined with cytokinin profiling of bacterial mutants revealed that the fas operon is essential for the enhanced production of isopentenyladenine, trans-zeatin, cis-zeatin, and the 2-methylthio derivatives of the zeatins. Cytokinin metabolite data and the demonstration of the enzymatic activities of FasD (isopentenyltransferase), FasE (cytokinin oxidase/dehydrogenase), and FasF (phosphoribohydrolase) led us to propose a pathway for the production of the cytokinin spectrum. Further evaluation of the pathogenicity of different fas mutants and of fas gene expression and cytokinin signal transduction upon infection implied that the secretion of the cytokinin mix is a highly dynamic process, with the consecutive production of a tom initiation wave followed by a maintenance flow.
- Keywords
- CORYNEBACTERIUM-FASCIANS, LEAFY GALL FORMATION, ENDOGENOUS CYTOKININS, TRANSFER-RNA, CIS-ZEATIN, ARABIDOPSIS, PROTEIN, GENES, IDENTIFICATION, BIOSYNTHESIS
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-1038865
- MLA
- Pertry, Ine, et al. “Rhodococcus Fascians Impacts Plant Development through the Dynamic Fas-Mediated Production of a Cytokinin Mix.” MOLECULAR PLANT-MICROBE INTERACTIONS, vol. 23, no. 9, 2010, pp. 1164–74, doi:10.1094/MPMI-23-9-1164.
- APA
- Pertry, I., Václavíková, K., Gemrotová, M., Spíchal, L., Galuszka, P., Depuydt, S., … Vereecke, D. (2010). Rhodococcus fascians impacts plant development through the dynamic Fas-mediated production of a cytokinin mix. MOLECULAR PLANT-MICROBE INTERACTIONS, 23(9), 1164–1174. https://doi.org/10.1094/MPMI-23-9-1164
- Chicago author-date
- Pertry, Ine, Katerina Václavíková, Markéta Gemrotová, Lukáš Spíchal, Petr Galuszka, Stephen Depuydt, Wim Temmerman, et al. 2010. “Rhodococcus Fascians Impacts Plant Development through the Dynamic Fas-Mediated Production of a Cytokinin Mix.” MOLECULAR PLANT-MICROBE INTERACTIONS 23 (9): 1164–74. https://doi.org/10.1094/MPMI-23-9-1164.
- Chicago author-date (all authors)
- Pertry, Ine, Katerina Václavíková, Markéta Gemrotová, Lukáš Spíchal, Petr Galuszka, Stephen Depuydt, Wim Temmerman, Elisabeth Stes, Annick De Keyser, Michael Riefler, Stefania Biondi, Ondrej Novak, Thomas Schmülling, Miroslav Strnad, Petr Tarkowski, Marcella Holsters, and Danny Vereecke. 2010. “Rhodococcus Fascians Impacts Plant Development through the Dynamic Fas-Mediated Production of a Cytokinin Mix.” MOLECULAR PLANT-MICROBE INTERACTIONS 23 (9): 1164–1174. doi:10.1094/MPMI-23-9-1164.
- Vancouver
- 1.Pertry I, Václavíková K, Gemrotová M, Spíchal L, Galuszka P, Depuydt S, et al. Rhodococcus fascians impacts plant development through the dynamic Fas-mediated production of a cytokinin mix. MOLECULAR PLANT-MICROBE INTERACTIONS. 2010;23(9):1164–74.
- IEEE
- [1]I. Pertry et al., “Rhodococcus fascians impacts plant development through the dynamic Fas-mediated production of a cytokinin mix,” MOLECULAR PLANT-MICROBE INTERACTIONS, vol. 23, no. 9, pp. 1164–1174, 2010.
@article{1038865, abstract = {{The phytopathogenic actinomycete Rhodococcus fascians D188 relies mainly on the linear plasmid-encoded fas operon for its virulence. The bacteria secrete six cytokinin bases that synergistically redirect the developmental program of the plant to stimulate proliferation of young shoot tissue, thus establishing a leafy gall as a niche. A yeast-based cytokinin bioassay combined with cytokinin profiling of bacterial mutants revealed that the fas operon is essential for the enhanced production of isopentenyladenine, trans-zeatin, cis-zeatin, and the 2-methylthio derivatives of the zeatins. Cytokinin metabolite data and the demonstration of the enzymatic activities of FasD (isopentenyltransferase), FasE (cytokinin oxidase/dehydrogenase), and FasF (phosphoribohydrolase) led us to propose a pathway for the production of the cytokinin spectrum. Further evaluation of the pathogenicity of different fas mutants and of fas gene expression and cytokinin signal transduction upon infection implied that the secretion of the cytokinin mix is a highly dynamic process, with the consecutive production of a tom initiation wave followed by a maintenance flow.}}, author = {{Pertry, Ine and Václavíková, Katerina and Gemrotová, Markéta and Spíchal, Lukáš and Galuszka, Petr and Depuydt, Stephen and Temmerman, Wim and Stes, Elisabeth and De Keyser, Annick and Riefler, Michael and Biondi, Stefania and Novak, Ondrej and Schmülling, Thomas and Strnad, Miroslav and Tarkowski, Petr and Holsters, Marcella and Vereecke, Danny}}, issn = {{0894-0282}}, journal = {{MOLECULAR PLANT-MICROBE INTERACTIONS}}, keywords = {{CORYNEBACTERIUM-FASCIANS,LEAFY GALL FORMATION,ENDOGENOUS CYTOKININS,TRANSFER-RNA,CIS-ZEATIN,ARABIDOPSIS,PROTEIN,GENES,IDENTIFICATION,BIOSYNTHESIS}}, language = {{eng}}, number = {{9}}, pages = {{1164--1174}}, title = {{Rhodococcus fascians impacts plant development through the dynamic Fas-mediated production of a cytokinin mix}}, url = {{http://doi.org/10.1094/MPMI-23-9-1164}}, volume = {{23}}, year = {{2010}}, }
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