Ghent University Academic Bibliography

Advanced

Rhodococcus fascians impacts plant development through the dynamic Fas-mediated production of a cytokinin mix

Ine Pertry UGent, Katerina Václavíková, Markéta Gemrotová, Lukáš Spíchal, Petr Galuszka, Stephen Depuydt UGent, Wim Temmerman UGent, Elisabeth Stes, Annick De Keyser UGent, Michael Riefler, et al. (2010) MOLECULAR PLANT-MICROBE INTERACTIONS. 23(9). p.1164-1174
abstract
The phytopathogenic actinomycete Rhodococcus fascians D188 relies mainly on the linear plasmid-encoded fas operon for its virulence. The bacteria secrete six cytokinin bases that synergistically redirect the developmental program of the plant to stimulate proliferation of young shoot tissue, thus establishing a leafy gall as a niche. A yeast-based cytokinin bioassay combined with cytokinin profiling of bacterial mutants revealed that the fas operon is essential for the enhanced production of isopentenyladenine, trans-zeatin, cis-zeatin, and the 2-methylthio derivatives of the zeatins. Cytokinin metabolite data and the demonstration of the enzymatic activities of FasD (isopentenyltransferase), FasE (cytokinin oxidase/dehydrogenase), and FasF (phosphoribohydrolase) led us to propose a pathway for the production of the cytokinin spectrum. Further evaluation of the pathogenicity of different fas mutants and of fas gene expression and cytokinin signal transduction upon infection implied that the secretion of the cytokinin mix is a highly dynamic process, with the consecutive production of a tom initiation wave followed by a maintenance flow.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
CORYNEBACTERIUM-FASCIANS, LEAFY GALL FORMATION, ENDOGENOUS CYTOKININS, TRANSFER-RNA, CIS-ZEATIN, ARABIDOPSIS, PROTEIN, GENES, IDENTIFICATION, BIOSYNTHESIS
journal title
MOLECULAR PLANT-MICROBE INTERACTIONS
Mol. Plant-Microbe Interact.
volume
23
issue
9
pages
1164 - 1174
Web of Science type
Article
Web of Science id
000281013600006
JCR category
PLANT SCIENCES
JCR impact factor
4.01 (2010)
JCR rank
18/185 (2010)
JCR quartile
1 (2010)
ISSN
0894-0282
DOI
10.1094/MPMI-23-9-1164
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1038865
handle
http://hdl.handle.net/1854/LU-1038865
date created
2010-09-08 19:13:12
date last changed
2016-12-19 15:44:33
@article{1038865,
  abstract     = {The phytopathogenic actinomycete Rhodococcus fascians D188 relies mainly on the linear plasmid-encoded fas operon for its virulence. The bacteria secrete six cytokinin bases that synergistically redirect the developmental program of the plant to stimulate proliferation of young shoot tissue, thus establishing a leafy gall as a niche. A yeast-based cytokinin bioassay combined with cytokinin profiling of bacterial mutants revealed that the fas operon is essential for the enhanced production of isopentenyladenine, trans-zeatin, cis-zeatin, and the 2-methylthio derivatives of the zeatins. Cytokinin metabolite data and the demonstration of the enzymatic activities of FasD (isopentenyltransferase), FasE (cytokinin oxidase/dehydrogenase), and FasF (phosphoribohydrolase) led us to propose a pathway for the production of the cytokinin spectrum. Further evaluation of the pathogenicity of different fas mutants and of fas gene expression and cytokinin signal transduction upon infection implied that the secretion of the cytokinin mix is a highly dynamic process, with the consecutive production of a tom initiation wave followed by a maintenance flow.},
  author       = {Pertry, Ine and V{\'a}clav{\'i}kov{\'a}, Katerina and Gemrotov{\'a}, Mark{\'e}ta and Sp{\'i}chal, Luk{\'a}\v{s} and Galuszka, Petr and Depuydt, Stephen and Temmerman, Wim and Stes, Elisabeth and De Keyser, Annick and Riefler, Michael and Biondi, Stefania and Novak, Ondrej and Schm{\"u}lling, Thomas and Strnad, Miroslav and Tarkowski, Petr and Holsters, Marcella and Vereecke, Danny},
  issn         = {0894-0282},
  journal      = {MOLECULAR PLANT-MICROBE INTERACTIONS},
  keyword      = {CORYNEBACTERIUM-FASCIANS,LEAFY GALL FORMATION,ENDOGENOUS CYTOKININS,TRANSFER-RNA,CIS-ZEATIN,ARABIDOPSIS,PROTEIN,GENES,IDENTIFICATION,BIOSYNTHESIS},
  language     = {eng},
  number       = {9},
  pages        = {1164--1174},
  title        = {Rhodococcus fascians impacts plant development through the dynamic Fas-mediated production of a cytokinin mix},
  url          = {http://dx.doi.org/10.1094/MPMI-23-9-1164},
  volume       = {23},
  year         = {2010},
}

Chicago
Pertry, Ine, Katerina Václavíková, Markéta Gemrotová, Lukáš Spíchal, Petr Galuszka, Stephen Depuydt, Wim Temmerman, et al. 2010. “Rhodococcus Fascians Impacts Plant Development Through the Dynamic Fas-mediated Production of a Cytokinin Mix.” Molecular Plant-microbe Interactions 23 (9): 1164–1174.
APA
Pertry, I., Václavíková, K., Gemrotová, M., Spíchal, L., Galuszka, P., Depuydt, S., Temmerman, W., et al. (2010). Rhodococcus fascians impacts plant development through the dynamic Fas-mediated production of a cytokinin mix. MOLECULAR PLANT-MICROBE INTERACTIONS, 23(9), 1164–1174.
Vancouver
1.
Pertry I, Václavíková K, Gemrotová M, Spíchal L, Galuszka P, Depuydt S, et al. Rhodococcus fascians impacts plant development through the dynamic Fas-mediated production of a cytokinin mix. MOLECULAR PLANT-MICROBE INTERACTIONS. 2010;23(9):1164–74.
MLA
Pertry, Ine, Katerina Václavíková, Markéta Gemrotová, et al. “Rhodococcus Fascians Impacts Plant Development Through the Dynamic Fas-mediated Production of a Cytokinin Mix.” MOLECULAR PLANT-MICROBE INTERACTIONS 23.9 (2010): 1164–1174. Print.