- Author
- Kris Morreel (UGent) , Oana Dima (UGent) , Hoon Kim, Fachuang Lu, Claudiu Niculaes (UGent) , Ruben Vanholme (UGent) , Rebecca Dauwe (UGent) , Geert Goeminne (UGent) , Dirk Inzé (UGent) , Eric Messens (UGent) , John Ralph and Wout Boerjan (UGent)
- Organization
- Abstract
- Although the primary structure of proteins, nucleic acids, and carbohydrates can be readily determined, no sequencing method has been described yet for the second most abundant biopolymer on earth (i.e. lignin). Within secondary-thickened plant cell walls, lignin forms an aromatic mesh arising from the combinatorial radical-radical coupling of monolignols and many other less abundant monomers. This polymerization process leads to a plethora of units and linkage types that affect the physicochemical characteristics of the cell wall. Current methods to analyze the lignin structure focus only on the frequency of the major monomeric units and interunit linkage types but do not provide information on the presence of less abundant unknown units and linkage types, nor on how linkages affect the formation of neighboring linkages. Such information can only be obtained using a sequencing approach. Here, we describe, to our knowledge for the first time, a sequencing strategy for lignin oligomers using mass spectrometry. This strategy was then evaluated on the oligomers extracted from wild-type poplar (Populus tremula x Populus tremuloides) xylem. In total, 134 lignin trimers to hexamers were observed, of which 36 could be completely sequenced. Interestingly, based on molecular mass data of the unknown monomeric and dimeric substructures, at least 10 unknown monomeric units or interunit linkage types were observed, one of which was identified as an arylglycerol end unit.
- Keywords
- FRAGMENTATIONS, NMR, LIGNIFICATION, BIOSYNTHESIS, REVEALS, CELL-WALLS, STRUCTURAL UNITS, POPLAR, ARABIDOPSIS-THALIANA, OLIGOLIGNOLS
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-1038703
- MLA
- Morreel, Kris, et al. “Mass Spectrometry-Based Sequencing of Lignin Oligomers.” PLANT PHYSIOLOGY, vol. 153, no. 4, 2010, pp. 1464–78, doi:10.1104/pp.110.156489.
- APA
- Morreel, K., Dima, O., Kim, H., Lu, F., Niculaes, C., Vanholme, R., … Boerjan, W. (2010). Mass spectrometry-based sequencing of lignin oligomers. PLANT PHYSIOLOGY, 153(4), 1464–1478. https://doi.org/10.1104/pp.110.156489
- Chicago author-date
- Morreel, Kris, Oana Dima, Hoon Kim, Fachuang Lu, Claudiu Niculaes, Ruben Vanholme, Rebecca Dauwe, et al. 2010. “Mass Spectrometry-Based Sequencing of Lignin Oligomers.” PLANT PHYSIOLOGY 153 (4): 1464–78. https://doi.org/10.1104/pp.110.156489.
- Chicago author-date (all authors)
- Morreel, Kris, Oana Dima, Hoon Kim, Fachuang Lu, Claudiu Niculaes, Ruben Vanholme, Rebecca Dauwe, Geert Goeminne, Dirk Inzé, Eric Messens, John Ralph, and Wout Boerjan. 2010. “Mass Spectrometry-Based Sequencing of Lignin Oligomers.” PLANT PHYSIOLOGY 153 (4): 1464–1478. doi:10.1104/pp.110.156489.
- Vancouver
- 1.Morreel K, Dima O, Kim H, Lu F, Niculaes C, Vanholme R, et al. Mass spectrometry-based sequencing of lignin oligomers. PLANT PHYSIOLOGY. 2010;153(4):1464–78.
- IEEE
- [1]K. Morreel et al., “Mass spectrometry-based sequencing of lignin oligomers,” PLANT PHYSIOLOGY, vol. 153, no. 4, pp. 1464–1478, 2010.
@article{1038703,
abstract = {{Although the primary structure of proteins, nucleic acids, and carbohydrates can be readily determined, no sequencing method has been described yet for the second most abundant biopolymer on earth (i.e. lignin). Within secondary-thickened plant cell walls, lignin forms an aromatic mesh arising from the combinatorial radical-radical coupling of monolignols and many other less abundant monomers. This polymerization process leads to a plethora of units and linkage types that affect the physicochemical characteristics of the cell wall. Current methods to analyze the lignin structure focus only on the frequency of the major monomeric units and interunit linkage types but do not provide information on the presence of less abundant unknown units and linkage types, nor on how linkages affect the formation of neighboring linkages. Such information can only be obtained using a sequencing approach. Here, we describe, to our knowledge for the first time, a sequencing strategy for lignin oligomers using mass spectrometry. This strategy was then evaluated on the oligomers extracted from wild-type poplar (Populus tremula x Populus tremuloides) xylem. In total, 134 lignin trimers to hexamers were observed, of which 36 could be completely sequenced. Interestingly, based on molecular mass data of the unknown monomeric and dimeric substructures, at least 10 unknown monomeric units or interunit linkage types were observed, one of which was identified as an arylglycerol end unit.}},
author = {{Morreel, Kris and Dima, Oana and Kim, Hoon and Lu, Fachuang and Niculaes, Claudiu and Vanholme, Ruben and Dauwe, Rebecca and Goeminne, Geert and Inzé, Dirk and Messens, Eric and Ralph, John and Boerjan, Wout}},
issn = {{0032-0889}},
journal = {{PLANT PHYSIOLOGY}},
keywords = {{FRAGMENTATIONS,NMR,LIGNIFICATION,BIOSYNTHESIS,REVEALS,CELL-WALLS,STRUCTURAL UNITS,POPLAR,ARABIDOPSIS-THALIANA,OLIGOLIGNOLS}},
language = {{eng}},
number = {{4}},
pages = {{1464--1478}},
title = {{Mass spectrometry-based sequencing of lignin oligomers}},
url = {{http://doi.org/10.1104/pp.110.156489}},
volume = {{153}},
year = {{2010}},
}
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