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A multiparameter approach to monitor disease activity in collagen-induced arthritis

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Abstract
Introduction: Disease severity in collagen-induced arthritis (CIA) is commonly assessed by clinical scoring of paw swelling and histological examination of joints. Although this is an accurate approach, it is also labour-intensive and the application of less invasive and less time-consuming methods is of great interest. However, it is still unclear which of these methods represents the most discriminating measure of disease activity. Methods: We undertook a comparative analysis in which different measurements of inflammation and tissue damage in CIA were studied on an individual mouse level. We compared the current gold standard methods - clinical scoring and histological examination - with alternative methods based on scoring of X-ray or micro-computed tomography (CT) images and investigated the significance of systemically expressed proteins, involved in CIA pathogenesis, that have potential as biomarkers. Results: Linear regression analysis revealed a marked association of serum matrix metalloproteinase (MMP)-3 levels with all features of CIA including inflammation, cartilage destruction and bone erosions. This association was improved by combined detection of MMP-3 and anti-collagen IgG2a antibody concentrations. In addition, combined analysis of both X-ray and micro-CT images was found to be predictive for cartilage and bone damage. Most remarkably, validation analysis using an independent data set proved that variations in disease severity, induced by different therapies, could be accurately represented by predicted values based on the proposed parameters. Conclusions: Our analyses revealed that clinical scoring, combined with serum MMP-3, anti-collagen IgG2a measurement and scoring of X-ray and micro-CT images, yields a comprehensive insight into the different aspects of disease activity in CIA.
Keywords
scoring methods, Collagen induced Arthritis, serum biomarkers

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Chicago
Seeuws, Sylvie, Peggy Jacques, Jens Van Praet, Michael Drennan, Julie Coudenys, Tine Decruy, Ellen Deschepper, et al. 2010. “A Multiparameter Approach to Monitor Disease Activity in Collagen-induced Arthritis.” Arthritis Research & Therapy 12 (4).
APA
Seeuws, S., Jacques, P., Van Praet, J., Drennan, M., Coudenys, J., Decruy, T., Deschepper, E., et al. (2010). A multiparameter approach to monitor disease activity in collagen-induced arthritis. ARTHRITIS RESEARCH & THERAPY, 12(4).
Vancouver
1.
Seeuws S, Jacques P, Van Praet J, Drennan M, Coudenys J, Decruy T, et al. A multiparameter approach to monitor disease activity in collagen-induced arthritis. ARTHRITIS RESEARCH & THERAPY. 2010;12(4).
MLA
Seeuws, Sylvie, Peggy Jacques, Jens Van Praet, et al. “A Multiparameter Approach to Monitor Disease Activity in Collagen-induced Arthritis.” ARTHRITIS RESEARCH & THERAPY 12.4 (2010): n. pag. Print.
@article{1038394,
  abstract     = {Introduction: Disease severity in collagen-induced arthritis (CIA) is commonly assessed by clinical scoring of paw swelling and histological examination of joints. Although this is an accurate approach, it is also labour-intensive and the application of less invasive and less time-consuming methods is of great interest. However, it is still unclear which of these methods represents the most discriminating measure of disease activity. 
Methods: We undertook a comparative analysis in which different measurements of inflammation and tissue damage in CIA were studied on an individual mouse level. We compared the current gold standard methods - clinical scoring and histological examination - with alternative methods based on scoring of X-ray or micro-computed tomography (CT) images and investigated the significance of systemically expressed proteins, involved in CIA pathogenesis, that have potential as biomarkers.
Results: Linear regression analysis revealed a marked association of serum matrix metalloproteinase (MMP)-3 levels with all features of CIA including inflammation, cartilage destruction and bone erosions. This association was improved by combined detection of MMP-3 and anti-collagen IgG2a antibody concentrations. In addition, combined analysis of both X-ray and micro-CT images was found to be predictive for cartilage and bone damage. Most remarkably, validation analysis using an independent data set proved that variations in disease severity, induced by different therapies, could be accurately represented by predicted values based on the proposed parameters.
Conclusions: Our analyses revealed that clinical scoring, combined with serum MMP-3, anti-collagen IgG2a measurement and scoring of X-ray and micro-CT images, yields a comprehensive insight into the different aspects of disease activity in CIA.},
  articleno    = {R160},
  author       = {Seeuws, Sylvie and Jacques, Peggy and Van Praet, Jens and Drennan, Michael and Coudenys, Julie and Decruy, Tine and Deschepper, Ellen and Lepescheux, Lien  and Pujuguet, Philippe and Oste, Line and Vandeghinste, Nick and Brys, Reginald and Verbruggen, August and Elewaut, Dirk},
  issn         = {1478-6362},
  journal      = {ARTHRITIS RESEARCH \& THERAPY},
  keyword      = {scoring methods,Collagen induced Arthritis,serum biomarkers},
  language     = {eng},
  number       = {4},
  pages        = {10},
  title        = {A multiparameter approach to monitor disease activity in collagen-induced arthritis},
  url          = {http://dx.doi.org/10.1186/ar3119},
  volume       = {12},
  year         = {2010},
}

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