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HPLC-MS characterisation of chelate modified somatropin

Evelien Wynendaele UGent, Ewald Pauwels UGent, Sylvia Van Dorpe UGent, Valentijn Vergote UGent, Christophe Van De Wiele UGent and Bart De Spiegeleer UGent (2010) Vlaams Jongerencongres van de Chemie, 10e, Abstracts.
abstract
Somatropin is a recombinant human growth hormone, consisting of 191 amino acids. This protein is clinically used in children and adults with inadequate endogenous growth hormone to stimulate a normal bone and muscle growth. In addition, somatropin is currently being investigated for the diagnosis and radiotherapy of certain hormonal cancers. The modification of the protein with the chelating agent NOTA (1,4,7-triazacyclononane-1,4,7-triacetic acid) allows the inclusion of metals coupled to the protein for diagnostic (e.g. 68Ga) or therapeutic (e.g. 90Y) purposes. The NOTA unit is selectively introduced on a lysine side chain. This yields 9 possible labelling sites for somatropin: FPTIPLSRLFDNAMLRAHRLHQLAFDTYQEFEEAYIPKEQKYSFLQNPQTSLCFSESIPTPSNREETQQKSNLELLRISLLLIQSWLEPVQFLRSVFANSLVYGASDSNVYDLLKDLEEGIQTLMGRLEDGSPRTGQIFKQTYSKFDTNSHNDDALLKNYGLLYCFRKDMDKVETFLRIVQCRSVEGSCGF We have applied an enzymatic digestion procedure for the characterisation of the modified somatropin, using trypsin, chymotrypsin and Staphylococcus aureus V-8 proteases. The resulting peptides were then monitored using HPLC-MS2, allowing the characterisation of the modified protein.
Please use this url to cite or link to this publication:
author
organization
year
type
conference
publication status
published
subject
keyword
Somatropin, NOTA, Peptide mapping
in
Vlaams Jongerencongres van de Chemie, 10e, Abstracts
publisher
Koninklijke Vlaamse Chemische Vereniging (KVCV)
conference name
10e Vlaams Jongerencongres van de Chemie (VJC 10)
conference location
Blankenberge
conference start
2010-03-01
conference end
2010-03-02
project
HPC-UGent: the central High Performance Computing infrastructure of Ghent University
language
English
UGent publication?
yes
classification
C3
copyright statement
I have retained and own the full copyright for this publication
id
1038333
handle
http://hdl.handle.net/1854/LU-1038333
date created
2010-09-08 07:32:04
date last changed
2013-09-17 10:50:02
@inproceedings{1038333,
  abstract     = {Somatropin is a recombinant human growth hormone, consisting of 191 amino acids. This protein is clinically used in children and adults with inadequate endogenous growth hormone to stimulate a normal bone and muscle growth. 
In addition, somatropin is currently being investigated for the diagnosis and radiotherapy of certain hormonal cancers. The modification of the protein with the chelating agent NOTA (1,4,7-triazacyclononane-1,4,7-triacetic acid) allows the inclusion of metals coupled to the protein for diagnostic (e.g. 68Ga) or therapeutic (e.g. 90Y) purposes. The NOTA unit is selectively introduced on a lysine side chain. This yields 9 possible labelling sites for somatropin:
FPTIPLSRLFDNAMLRAHRLHQLAFDTYQEFEEAYIPKEQKYSFLQNPQTSLCFSESIPTPSNREETQQKSNLELLRISLLLIQSWLEPVQFLRSVFANSLVYGASDSNVYDLLKDLEEGIQTLMGRLEDGSPRTGQIFKQTYSKFDTNSHNDDALLKNYGLLYCFRKDMDKVETFLRIVQCRSVEGSCGF
We have applied an enzymatic digestion procedure for the characterisation of the modified somatropin, using trypsin, chymotrypsin and Staphylococcus aureus V-8 proteases. The resulting peptides were then monitored using HPLC-MS2, allowing the characterisation of the modified protein.},
  author       = {Wynendaele, Evelien and Pauwels, Ewald and Van Dorpe, Sylvia and Vergote, Valentijn and Van De Wiele, Christophe and De Spiegeleer, Bart},
  booktitle    = {Vlaams Jongerencongres van de Chemie, 10e, Abstracts},
  keyword      = {Somatropin,NOTA,Peptide mapping},
  language     = {eng},
  location     = {Blankenberge},
  publisher    = {Koninklijke Vlaamse Chemische Vereniging (KVCV)},
  title        = {HPLC-MS characterisation of chelate modified somatropin},
  year         = {2010},
}

Chicago
Wynendaele, Evelien, Ewald Pauwels, Sylvia Van Dorpe, Valentijn Vergote, Christophe Van De Wiele, and Bart De Spiegeleer. 2010. “HPLC-MS Characterisation of Chelate Modified Somatropin.” In Vlaams Jongerencongres Van De Chemie, 10e, Abstracts. Koninklijke Vlaamse Chemische Vereniging (KVCV).
APA
Wynendaele, E., Pauwels, E., Van Dorpe, S., Vergote, V., Van De Wiele, C., & De Spiegeleer, B. (2010). HPLC-MS characterisation of chelate modified somatropin. Vlaams Jongerencongres van de Chemie, 10e, Abstracts. Presented at the 10e Vlaams Jongerencongres van de Chemie (VJC 10), Koninklijke Vlaamse Chemische Vereniging (KVCV).
Vancouver
1.
Wynendaele E, Pauwels E, Van Dorpe S, Vergote V, Van De Wiele C, De Spiegeleer B. HPLC-MS characterisation of chelate modified somatropin. Vlaams Jongerencongres van de Chemie, 10e, Abstracts. Koninklijke Vlaamse Chemische Vereniging (KVCV); 2010.
MLA
Wynendaele, Evelien, Ewald Pauwels, Sylvia Van Dorpe, et al. “HPLC-MS Characterisation of Chelate Modified Somatropin.” Vlaams Jongerencongres Van De Chemie, 10e, Abstracts. Koninklijke Vlaamse Chemische Vereniging (KVCV), 2010. Print.