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Staphylococcus aureus enterotoxin B facilitates allergic sensitization in experimental asthma

WOUTER HUVENNE UGent, Ina Callebaut, Maud Plantinga UGent, Jeroen AJ Vanoirbeek, Olga Krysko UGent, Dominique MA Bullens, Philippe Gevaert UGent, Paul Van Cauwenberge UGent, Bart Lambrecht UGent and Jan L Ceuppens, et al. (2010) CLINICAL AND EXPERIMENTAL ALLERGY. 40(7). p.1079-1090
abstract
BACKGROUND: Staphylococcus aureus Enterotoxin B (SEB) has immunomodulatory effects in allergic airway disease. The potential contribution of SEB to the sensitization process to allergens remains obscure. OBJECTIVE: In order to study the effects of staphylococcal-derived toxins on the sensitization to ovalbumin (OVA) and induction of allergic airway inflammation, we have combined the nasal application of OVA with different toxins. METHODS: Nasal applications of OVA and saline, SEA, SEB, toxic shock syndrome toxin (TSST)-1, protein A or lipopolysaccharide (LPS) were performed on alternate days from day 0 till 12. On day 14, mice were killed for the evaluation of OVA-specific IgE, cytokine production by mediastinal lymph node (MLN) cells and bronchial hyperreactivity (BHR) to inhaled metacholine. The effect of SEB on dendritic cell (DC) migration and maturation, and on T cell proliferation was evaluated. RESULTS: Concomitant endonasal application of OVA and SEB resulted in OVA-specific IgE production, whereas this was not found with SEA, TSST-1, protein A, LPS or OVA alone. Increased DC maturation and migration to the draining lymph nodes were observed in OVA/SEB mice, as well as an increased T cell proliferation. Bronchial inflammation with an influx of eosinophils and lymphocytes was demonstrated in OVA/SEB mice, together with hyperresponsiveness and the production of IL-4, IL-5, IL-10 and IL-13 by MLN stimulated with OVA. CONCLUSIONS: Our data demonstrate that SEB facilitates sensitization to OVA and consecutive bronchial inflammation with features of allergic asthma. This is likely due to augmentation of DC migration and maturation, as well as the allergen-specific T cell proliferation upon concomitant OVA and SEB application.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
CD4 T cell, Staphylococcus aureus enterotoxin B, asthma, allergic sensitization, superantigens, DENDRITIC CELL MATURATION, AIRWAY INFLAMMATION, EOSINOPHILIC INFLAMMATION, BACTERIAL-INFECTIONS, ATOPIC-DERMATITIS, INHALED ANTIGEN, IGE ANTIBODIES, NASAL POLYPS, T-CELLS, MICE
journal title
CLINICAL AND EXPERIMENTAL ALLERGY
Clin. Exp. Allergy
volume
40
issue
7
pages
1079 - 1090
Web of Science type
Article
Web of Science id
000278645500016
JCR category
ALLERGY
JCR impact factor
4.195 (2010)
JCR rank
3/20 (2010)
JCR quartile
1 (2010)
ISSN
0954-7894
DOI
10.1111/j.1365-2222.2010.03464.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1035717
handle
http://hdl.handle.net/1854/LU-1035717
date created
2010-09-01 16:31:48
date last changed
2010-09-01 17:00:56
@article{1035717,
  abstract     = {BACKGROUND: Staphylococcus aureus Enterotoxin B (SEB) has immunomodulatory effects in allergic airway disease. The potential contribution of SEB to the sensitization process to allergens remains obscure.
OBJECTIVE: In order to study the effects of staphylococcal-derived toxins on the sensitization to ovalbumin (OVA) and induction of allergic airway inflammation, we have combined the nasal application of OVA with different toxins.
METHODS: Nasal applications of OVA and saline, SEA, SEB, toxic shock syndrome toxin (TSST)-1, protein A or lipopolysaccharide (LPS) were performed on alternate days from day 0 till 12. On day 14, mice were killed for the evaluation of OVA-specific IgE, cytokine production by mediastinal lymph node (MLN) cells and bronchial hyperreactivity (BHR) to inhaled metacholine. The effect of SEB on dendritic cell (DC) migration and maturation, and on T cell proliferation was evaluated.
RESULTS: Concomitant endonasal application of OVA and SEB resulted in OVA-specific IgE production, whereas this was not found with SEA, TSST-1, protein A, LPS or OVA alone. Increased DC maturation and migration to the draining lymph nodes were observed in OVA/SEB mice, as well as an increased T cell proliferation. Bronchial inflammation with an influx of eosinophils and lymphocytes was demonstrated in OVA/SEB mice, together with hyperresponsiveness and the production of IL-4, IL-5, IL-10 and IL-13 by MLN stimulated with OVA.
CONCLUSIONS: Our data demonstrate that SEB facilitates sensitization to OVA and consecutive bronchial inflammation with features of allergic asthma. This is likely due to augmentation of DC migration and maturation, as well as the allergen-specific T cell proliferation upon concomitant OVA and SEB application.},
  author       = {HUVENNE, WOUTER and Callebaut, Ina and Plantinga, Maud and Vanoirbeek, Jeroen AJ and Krysko, Olga and Bullens, Dominique MA and Gevaert, Philippe and Van Cauwenberge, Paul and Lambrecht, Bart and Ceuppens, Jan L and Bachert, Claus and Hellings, Peter W},
  issn         = {0954-7894},
  journal      = {CLINICAL AND EXPERIMENTAL ALLERGY},
  keyword      = {CD4 T cell,Staphylococcus aureus enterotoxin B,asthma,allergic sensitization,superantigens,DENDRITIC CELL MATURATION,AIRWAY INFLAMMATION,EOSINOPHILIC INFLAMMATION,BACTERIAL-INFECTIONS,ATOPIC-DERMATITIS,INHALED ANTIGEN,IGE ANTIBODIES,NASAL POLYPS,T-CELLS,MICE},
  language     = {eng},
  number       = {7},
  pages        = {1079--1090},
  title        = {Staphylococcus aureus enterotoxin B facilitates allergic sensitization in experimental asthma},
  url          = {http://dx.doi.org/10.1111/j.1365-2222.2010.03464.x},
  volume       = {40},
  year         = {2010},
}

Chicago
HUVENNE, WOUTER, Ina Callebaut, Maud Plantinga, Jeroen AJ Vanoirbeek, Olga Krysko, Dominique MA Bullens, Philippe Gevaert, et al. 2010. “Staphylococcus Aureus Enterotoxin B Facilitates Allergic Sensitization in Experimental Asthma.” Clinical and Experimental Allergy 40 (7): 1079–1090.
APA
HUVENNE, W., Callebaut, I., Plantinga, M., Vanoirbeek, J. A., Krysko, O., Bullens, D. M., Gevaert, P., et al. (2010). Staphylococcus aureus enterotoxin B facilitates allergic sensitization in experimental asthma. CLINICAL AND EXPERIMENTAL ALLERGY, 40(7), 1079–1090.
Vancouver
1.
HUVENNE W, Callebaut I, Plantinga M, Vanoirbeek JA, Krysko O, Bullens DM, et al. Staphylococcus aureus enterotoxin B facilitates allergic sensitization in experimental asthma. CLINICAL AND EXPERIMENTAL ALLERGY. 2010;40(7):1079–90.
MLA
HUVENNE, WOUTER, Ina Callebaut, Maud Plantinga, et al. “Staphylococcus Aureus Enterotoxin B Facilitates Allergic Sensitization in Experimental Asthma.” CLINICAL AND EXPERIMENTAL ALLERGY 40.7 (2010): 1079–1090. Print.