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P-cadherin in adhesion and invasion: opposite roles in colon and bladder carcinoma

(2011) INTERNATIONAL JOURNAL OF CANCER. 128(5). p.1031-1044
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Abstract
Neoexpression or upregulation of placental cadherin (P-cadherin), a member of the classical cadherin family, has previously been described in several carcinomas, such as colorectal and bladder carcinomas. In this study, we combined two different approaches, immunohistochemistry of tumor samples and in vitro knockdown of P-cadherin, to gain a better insight into the role of P-cadherin in these types of cancer. First, we performed immunohistochemistry for P- and E-cadherins in a series of 52 colorectal adenocarcinomas, including well, moderately and poorly differentiated (WD, MD, and PD) tumors. Decrease or loss of P-cadherin neoexpression was significantly associated with a higher tumor grade and could discriminate WD from MD and/or PD tumors (p < 0.001). E-cadherin, on the other hand, was strongly expressed at the membrane of most WD (18 of 19) and MD tumors (15 of 19). Downregulation correlated significantly with the PD phenotype (p < 0.001). In a second approach, we transiently or stably knocked down P-cadherin in HT-29 colon adenocarcinoma cells. This led to decreased intercellular adhesion and to an increased migratory and long-term invasive phenotype compared with control HT-29 cells, suggesting that P-cadherin acts as a proadhesive and anti-invasive/antimigratory molecule in colon carcinoma cells. Contrasting with these results and illustrating the context-specific function of P-cadherin were our results obtained in RT-112 bladder carcinoma cells. Stable knockdown of P-cadherin in RT-112 cells diminished invasion and migration, and promoted intercellular adhesion.
Keywords
invasion, bladder cancer, P-cadherin, adhesion, colorectal cancer, TRANSITIONAL-CELL-CARCINOMA, BREAST-CANCER CELLS, N-CADHERIN, COLORECTAL-CANCER, CATENIN EXPRESSION, PROGNOSTIC-SIGNIFICANCE, MESENCHYMAL TRANSITION, NEOPLASTIC PROGRESSION, GASTRIC CARCINOMAS, GROWTH-FACTOR

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Chicago
Van Marck, Veerle, Christophe Stove, Koen Jacobs, Gert Van den Eynden, and Marc Bracke. 2011. “P-cadherin in Adhesion and Invasion: Opposite Roles in Colon and Bladder Carcinoma.” International Journal of Cancer 128 (5): 1031–1044.
APA
Van Marck, V., Stove, C., Jacobs, K., Van den Eynden, G., & Bracke, M. (2011). P-cadherin in adhesion and invasion: opposite roles in colon and bladder carcinoma. INTERNATIONAL JOURNAL OF CANCER, 128(5), 1031–1044.
Vancouver
1.
Van Marck V, Stove C, Jacobs K, Van den Eynden G, Bracke M. P-cadherin in adhesion and invasion: opposite roles in colon and bladder carcinoma. INTERNATIONAL JOURNAL OF CANCER. 2011;128(5):1031–44.
MLA
Van Marck, Veerle, Christophe Stove, Koen Jacobs, et al. “P-cadherin in Adhesion and Invasion: Opposite Roles in Colon and Bladder Carcinoma.” INTERNATIONAL JOURNAL OF CANCER 128.5 (2011): 1031–1044. Print.
@article{1035686,
  abstract     = {Neoexpression or upregulation of placental cadherin (P-cadherin), a member of the classical cadherin family, has previously been described in several carcinomas, such as colorectal and bladder carcinomas. In this study, we combined two different approaches, immunohistochemistry of tumor samples and in vitro knockdown of P-cadherin, to gain a better insight into the role of P-cadherin in these types of cancer. First, we performed immunohistochemistry for P- and E-cadherins in a series of 52 colorectal adenocarcinomas, including well, moderately and poorly differentiated (WD, MD, and PD) tumors. Decrease or loss of P-cadherin neoexpression was significantly associated with a higher tumor grade and could discriminate WD from MD and/or PD tumors (p {\textlangle} 0.001). E-cadherin, on the other hand, was strongly expressed at the membrane of most WD (18 of 19) and MD tumors (15 of 19). Downregulation correlated significantly with the PD phenotype (p {\textlangle} 0.001). In a second approach, we transiently or stably knocked down P-cadherin in HT-29 colon adenocarcinoma cells. This led to decreased intercellular adhesion and to an increased migratory and long-term invasive phenotype compared with control HT-29 cells, suggesting that P-cadherin acts as a proadhesive and anti-invasive/antimigratory molecule in colon carcinoma cells. Contrasting with these results and illustrating the context-specific function of P-cadherin were our results obtained in RT-112 bladder carcinoma cells. Stable knockdown of P-cadherin in RT-112 cells diminished invasion and migration, and promoted intercellular adhesion.},
  author       = {Van Marck, Veerle and Stove, Christophe and Jacobs, Koen and Van den Eynden, Gert and Bracke, Marc},
  issn         = {0020-7136},
  journal      = {INTERNATIONAL JOURNAL OF CANCER},
  keyword      = {invasion,bladder cancer,P-cadherin,adhesion,colorectal cancer,TRANSITIONAL-CELL-CARCINOMA,BREAST-CANCER CELLS,N-CADHERIN,COLORECTAL-CANCER,CATENIN EXPRESSION,PROGNOSTIC-SIGNIFICANCE,MESENCHYMAL TRANSITION,NEOPLASTIC PROGRESSION,GASTRIC CARCINOMAS,GROWTH-FACTOR},
  language     = {eng},
  number       = {5},
  pages        = {1031--1044},
  title        = {P-cadherin in adhesion and invasion: opposite roles in colon and bladder carcinoma},
  url          = {http://dx.doi.org/10.1002/ijc.25427},
  volume       = {128},
  year         = {2011},
}

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