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Alteration of N-glycome in diethylnitrosamine-induced hepatocellular carcinoma mice: a non-invasive monitoring tool for liver cancer

Xue-en Liu, Sylviane Dewaele UGent, Valerie Vanhooren UGent, Yedong Fan UGent, Ling Wang, Jacques Van Huysse UGent, Hui Zhuang, Roland Contreras UGent, Claude Libert UGent and Cuiying Chen UGent (2010) LIVER INTERNATIONAL. 30(8). p.1221-1228
abstract
Background and aims: There is a demand for serum markers that can routinely assess the progression of liver cancer. DENA (diethylnitrosamine), a hepatocarcinogen, is commonly used in an experimental mouse model to induce liver cancer that closely mimics a subclass of human hepatocellular carcinoma (HCC). However, blood monitoring of the progression of HCC in mouse model has not yet been achieved. In this report, we studied glycomics during the development of mouse HCC induced by DENA. Methods: Mouse HCC was induced by DENA. Serum N-glycans were profiled using the sequencer assisted–Fluorophore-assisted carbohydrate electrophoresis technique developed in our laboratory. Possible alteration in the transcription of genes relevant to the synthesis of the changed glycans was analysed by realtime polymerase chain reaction. Results: In comparison with the control mice that received the same volume of saline, a tri-antennary glycan (peak 8) and a biantennary glycan (peak 4) in serum total glycans of DENA mice increased gradually but significantly during progression of liver cancer, whereas a corefucosylated biantennary glycan (peak 6) decreased. Expression of a-1,6- fucosyltransferase 8 (Fut8), which is responsible for core fucosylation, decreased in the liver of DENA mice compared with that of age-matched control mice. Likewise, the expression level of Mgat4a, which is responsible for tri-antennary, significantly increased in the liver of DENA mice (Po0.001). Conclusions: The changes of N-glycan levels in the serum could be used as a biomarker to monitor the progress of HCC and to follow up the treatment of liver tumours in this DENA mouse model.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
ACETYLGLUCOSAMINYLTRANSFERASE-III, MOUSE MODELS, SERUM-PROTEIN GLYCOMICS, N-glycan, marker, hepatocellular carcinoma (HCC), DENA, GLYCOSYLATION, DNA, RATS, EXPRESSION, HEPATOMA, GLYCANS, DISEASE
journal title
LIVER INTERNATIONAL
Liver Int.
volume
30
issue
8
pages
1221 - 1228
Web of Science type
Article
Web of Science id
000280666800020
JCR category
GASTROENTEROLOGY & HEPATOLOGY
JCR impact factor
3.84 (2010)
JCR rank
16/71 (2010)
JCR quartile
1 (2010)
ISSN
1478-3223
DOI
10.1111/j.1478-3231.2010.02279.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1029782
handle
http://hdl.handle.net/1854/LU-1029782
date created
2010-08-30 10:27:00
date last changed
2012-06-26 14:32:06
@article{1029782,
  abstract     = {Background and aims: There is a demand for serum markers that can routinely assess the progression of liver cancer. DENA (diethylnitrosamine), a hepatocarcinogen, is commonly used in an experimental mouse model to induce liver cancer that closely mimics a subclass of human hepatocellular carcinoma (HCC). However, blood monitoring of the progression of HCC in mouse model has not yet been achieved. In this report, we studied glycomics during the development of mouse HCC induced by DENA.
Methods: Mouse HCC was induced by DENA. Serum N-glycans were profiled using the sequencer assisted--Fluorophore-assisted carbohydrate electrophoresis technique developed in our laboratory. Possible alteration in the transcription of genes relevant to the synthesis of the changed glycans was analysed by realtime polymerase chain reaction. Results: In comparison with the control mice that received the same volume of saline, a tri-antennary glycan (peak 8) and a biantennary glycan (peak 4) in serum total glycans of DENA mice increased gradually but significantly during progression of liver cancer, whereas a corefucosylated biantennary glycan (peak 6) decreased. Expression of a-1,6- fucosyltransferase 8 (Fut8), which is responsible for core fucosylation, decreased in the liver of DENA mice compared with that of age-matched control mice. Likewise, the expression level of Mgat4a, which is responsible for tri-antennary, significantly increased in the liver of DENA mice (Po0.001). Conclusions: The changes of N-glycan levels in the serum could be used as a biomarker to monitor the progress of HCC and to follow up the treatment of liver tumours in this DENA mouse model.},
  author       = {Liu, Xue-en and Dewaele, Sylviane and Vanhooren, Valerie and Fan, Yedong and Wang, Ling and Van Huysse, Jacques and Zhuang, Hui and Contreras, Roland and Libert, Claude and Chen, Cuiying},
  issn         = {1478-3223},
  journal      = {LIVER INTERNATIONAL},
  keyword      = {ACETYLGLUCOSAMINYLTRANSFERASE-III,MOUSE MODELS,SERUM-PROTEIN GLYCOMICS,N-glycan,marker,hepatocellular carcinoma (HCC),DENA,GLYCOSYLATION,DNA,RATS,EXPRESSION,HEPATOMA,GLYCANS,DISEASE},
  language     = {eng},
  number       = {8},
  pages        = {1221--1228},
  title        = {Alteration of N-glycome in diethylnitrosamine-induced hepatocellular carcinoma mice: a non-invasive monitoring tool for liver cancer},
  url          = {http://dx.doi.org/10.1111/j.1478-3231.2010.02279.x},
  volume       = {30},
  year         = {2010},
}

Chicago
Liu, Xue-en, Sylviane Dewaele, Valerie Vanhooren, Yedong Fan, Ling Wang, Jacques Van Huysse, Hui Zhuang, Roland Contreras, Claude Libert, and Cuiying Chen. 2010. “Alteration of N-glycome in Diethylnitrosamine-induced Hepatocellular Carcinoma Mice: a Non-invasive Monitoring Tool for Liver Cancer.” Liver International 30 (8): 1221–1228.
APA
Liu, Xue-en, Dewaele, S., Vanhooren, V., Fan, Y., Wang, L., Van Huysse, J., Zhuang, H., et al. (2010). Alteration of N-glycome in diethylnitrosamine-induced hepatocellular carcinoma mice: a non-invasive monitoring tool for liver cancer. LIVER INTERNATIONAL, 30(8), 1221–1228.
Vancouver
1.
Liu X, Dewaele S, Vanhooren V, Fan Y, Wang L, Van Huysse J, et al. Alteration of N-glycome in diethylnitrosamine-induced hepatocellular carcinoma mice: a non-invasive monitoring tool for liver cancer. LIVER INTERNATIONAL. 2010;30(8):1221–8.
MLA
Liu, Xue-en, Sylviane Dewaele, Valerie Vanhooren, et al. “Alteration of N-glycome in Diethylnitrosamine-induced Hepatocellular Carcinoma Mice: a Non-invasive Monitoring Tool for Liver Cancer.” LIVER INTERNATIONAL 30.8 (2010): 1221–1228. Print.