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Validation of the eta-s.t.a.r. 1+1 for rapid screening of residues of -lactam antibiotics in milk

Wim Reybroeck, Sigrid Ooghe, Hubert De Brabander UGent and Els Daeseleire (2010) FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT. 27(8). p.1084-1095
abstract
The 2-min protocol (1 + 1) for the eta-s.t.a.r. (manufactured by Neogen Corporation, Lansing, MI, USA) was validated at the Technology and Food Science Unit of the Institute for Agricultural and Fisheries Research according to Commission Decision 2002/657/EC. The test was very selective for the group of -lactam compounds: the only interference found was by clavulanic acid at 2500 mu g kg-1 and above. The modified protocol (eta-s.t.a.r. 1 + 1) detected all -lactams with a maximum residue limit (MRL) in milk, but not all these compounds were detected at their respective MRL. The detection of cefalexin (detection capability = 6000 mu g kg-1; MRL = 100 mu g kg-1) and penethamate (detection capability = 80 mu g kg-1; MRL = 4 mu g kg-1) was especially poor, and also ceftiofur was only detected from 500 mu g kg-1 (MRL = 100 mu g kg-1). The repeatability of the reader and of the test was very good. The test was very robust: test results were not significantly influenced by small changes in the test protocol, by the milk composition or by the type of milk. The test was also suitable to test the milk of animal species other than cow. Favourable results were obtained in testing monitoring samples, in two national ring trials, and in an international proficiency test. The eta-s.t.a.r. 1 + 1 is a very fast, simple, and reliable test that could be used at the farm level to prevent tanker milk contamination by -lactams.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
screening assays, in-house validation, veterinary drug residues, antibiotics, milk, PENICILLIN-G, INHIBITORS, TESTS, ASSAY
journal title
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
Food Addit. Contam. Part A-Chem.
volume
27
issue
8
pages
1084 - 1095
Web of Science type
Article
Web of Science id
000279331700004
JCR category
FOOD SCIENCE & TECHNOLOGY
JCR impact factor
2.23 (2010)
JCR rank
24/124 (2010)
JCR quartile
1 (2010)
ISSN
1944-0049
DOI
10.1080/19440041003724871
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1029295
handle
http://hdl.handle.net/1854/LU-1029295
date created
2010-08-27 11:32:43
date last changed
2016-12-19 15:44:31
@article{1029295,
  abstract     = {The 2-min protocol (1 + 1) for the eta-s.t.a.r. (manufactured by Neogen Corporation, Lansing, MI, USA) was validated at the Technology and Food Science Unit of the Institute for Agricultural and Fisheries Research according to Commission Decision 2002/657/EC. The test was very selective for the group of -lactam compounds: the only interference found was by clavulanic acid at 2500 mu g kg-1 and above. The modified protocol (eta-s.t.a.r. 1 + 1) detected all -lactams with a maximum residue limit (MRL) in milk, but not all these compounds were detected at their respective MRL. The detection of cefalexin (detection capability = 6000 mu g kg-1; MRL = 100 mu g kg-1) and penethamate (detection capability = 80 mu g kg-1; MRL = 4 mu g kg-1) was especially poor, and also ceftiofur was only detected from 500 mu g kg-1 (MRL = 100 mu g kg-1). The repeatability of the reader and of the test was very good. The test was very robust: test results were not significantly influenced by small changes in the test protocol, by the milk composition or by the type of milk. The test was also suitable to test the milk of animal species other than cow. Favourable results were obtained in testing monitoring samples, in two national ring trials, and in an international proficiency test. The eta-s.t.a.r. 1 + 1 is a very fast, simple, and reliable test that could be used at the farm level to prevent tanker milk contamination by -lactams.},
  author       = {Reybroeck, Wim and Ooghe, Sigrid and De Brabander, Hubert and Daeseleire, Els},
  issn         = {1944-0049},
  journal      = {FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE \& RISK ASSESSMENT},
  keyword      = {screening assays,in-house validation,veterinary drug residues,antibiotics,milk,PENICILLIN-G,INHIBITORS,TESTS,ASSAY},
  language     = {eng},
  number       = {8},
  pages        = {1084--1095},
  title        = {Validation of the eta-s.t.a.r. 1+1 for rapid screening of residues of -lactam antibiotics in milk},
  url          = {http://dx.doi.org/10.1080/19440041003724871},
  volume       = {27},
  year         = {2010},
}

Chicago
Reybroeck, Wim, Sigrid Ooghe, Hubert De Brabander, and Els Daeseleire. 2010. “Validation of the Eta-s.t.a.r. 1+1 for Rapid Screening of Residues of -lactam Antibiotics in Milk.” Food Additives and Contaminants Part A-chemistry Analysis Control Exposure & Risk Assessment 27 (8): 1084–1095.
APA
Reybroeck, Wim, Ooghe, S., De Brabander, H., & Daeseleire, E. (2010). Validation of the eta-s.t.a.r. 1+1 for rapid screening of residues of -lactam antibiotics in milk. FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT, 27(8), 1084–1095.
Vancouver
1.
Reybroeck W, Ooghe S, De Brabander H, Daeseleire E. Validation of the eta-s.t.a.r. 1+1 for rapid screening of residues of -lactam antibiotics in milk. FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT. 2010;27(8):1084–95.
MLA
Reybroeck, Wim, Sigrid Ooghe, Hubert De Brabander, et al. “Validation of the Eta-s.t.a.r. 1+1 for Rapid Screening of Residues of -lactam Antibiotics in Milk.” FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT 27.8 (2010): 1084–1095. Print.