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Influence of sperm-oocyte coincubation period on porcine in vitro fertilization (IVF) efficiency

Josine Beek UGent, Dominiek Maes UGent, Hans Nauwynck UGent and Ann Van Soom UGent (2010) REPRODUCTION IN DOMESTIC ANIMALS. 45. p.78-78
abstract
A major obstacle for successful in vitro production of porcine embryos is the polyspermic fertilization. One possibility to reduce polyspermic penetration is decreasing the number of spermatozoa added to the fertilization medium. Unfortunately, the lower rate of polyspermy is accompanied by a reduced penetration rate. A short gamete coincubation period of 10 min has been described to obtain fertilization rates similar to 6 h of coincubation and may improve IVF efficiency (number of monospermic fertilized oocytes/total number inseminated) depending on sperm-oocyte ratio (Gil, 2007, Theriogenology, 67(3), 620–626). Here we demonstrate that the optimal coincubation period in our IVF conditions is between 10 min and 6 h. In vitro matured oocytes (n = 600) were inseminated with frozen-thawed epididymal semen with 600 spermatozoa per oocyte and coincubated for 2, 4 and 6 h. At 2 and 4 h post insemination (hpi), oocytes were vortexed and transferred to fertilization medium without spermatozoa. At 6 hpi, presumed zygotes of all groups were washed three times in culture medium and cultured. At 22 hpi, zygotes were fixed overnight and stained with Hoechst 33,342 for the assessment of fertilization and polyspermy. The IVF efficiency was higher for the 4 h group (40 ± 5%) than the 2 and 6 h group (19 ± 8% and 17 ± 5%). Between 4 and 6 h of gamete coincubation, the increase in the number of polyspermic oocytes was relatively higher than the increase in penetration rate (+39% vs. +15%), resulting in a decline in efficiency. (This study was supported by Research Foundation-Flanders).
Please use this url to cite or link to this publication:
author
organization
year
type
conference
publication status
published
subject
keyword
Influence of sperm-oocyte coincubation period on porcine in vitro fertilization (IVF) efficiency
in
REPRODUCTION IN DOMESTIC ANIMALS
Reprod. Domest. Anim.
volume
45
pages
1 pages
publisher
WILEY-BLACKWELL PUBLISHING, INC
place of publication
MALDEN
conference name
14th Annual Conference of theEuropean-Societies-Domestic-Animals-Reproduction/22nd Annual Meeting of the EU-AI-Vets
conference location
Eger, HUNGARY
conference start
2010-09-15
conference end
2010-09-22
Web of Science type
Meeting Abstract
Web of Science id
000280355200119
JCR category
VETERINARY SCIENCES
JCR impact factor
1.606 (2010)
JCR rank
26/145 (2010)
JCR quartile
1 (2010)
ISSN
0936-6768
DOI
10.1111/j.1439-0531.2010.01654_3.x
language
English
UGent publication?
yes
classification
C3
copyright statement
I don't know the status of the copyright for this publication
id
1026203
handle
http://hdl.handle.net/1854/LU-1026203
date created
2010-08-19 16:02:39
date last changed
2010-08-23 11:07:49
@inproceedings{1026203,
  abstract     = {A major obstacle for successful in vitro production of porcine embryos
is the polyspermic fertilization. One possibility to reduce polyspermic
penetration is decreasing the number of spermatozoa added to the
fertilization medium. Unfortunately, the lower rate of polyspermy is
accompanied by a reduced penetration rate. A short gamete coincubation
period of 10 min has been described to obtain fertilization rates
similar to 6 h of coincubation and may improve IVF efficiency
(number of monospermic fertilized oocytes/total number inseminated)
depending on sperm-oocyte ratio (Gil, 2007, Theriogenology, 67(3),
620--626). Here we demonstrate that the optimal coincubation period
in our IVF conditions is between 10 min and 6 h. In vitro matured
oocytes (n = 600) were inseminated with frozen-thawed epididymal
semen with 600 spermatozoa per oocyte and coincubated for 2, 4 and
6 h. At 2 and 4 h post insemination (hpi), oocytes were vortexed and
transferred to fertilization medium without spermatozoa. At 6 hpi,
presumed zygotes of all groups were washed three times in culture
medium and cultured. At 22 hpi, zygotes were fixed overnight and
stained with Hoechst 33,342 for the assessment of fertilization and
polyspermy. The IVF efficiency was higher for the 4 h group
(40 {\textpm} 5\%) than the 2 and 6 h group (19 {\textpm} 8\% and 17 {\textpm} 5\%).
Between 4 and 6 h of gamete coincubation, the increase in the number
of polyspermic oocytes was relatively higher than the increase in
penetration rate (+39\% vs. +15\%), resulting in a decline in efficiency.
(This study was supported by Research Foundation-Flanders).},
  author       = {Beek, Josine and Maes, Dominiek and Nauwynck, Hans and Van Soom, Ann},
  booktitle    = {REPRODUCTION IN DOMESTIC ANIMALS},
  issn         = {0936-6768},
  keyword      = {Influence of sperm-oocyte coincubation period on porcine in vitro fertilization (IVF) efficiency},
  language     = {eng},
  location     = {Eger, HUNGARY},
  pages        = {78--78},
  publisher    = {WILEY-BLACKWELL PUBLISHING, INC},
  title        = {Influence of sperm-oocyte coincubation period on porcine in vitro fertilization (IVF) efficiency},
  url          = {http://dx.doi.org/10.1111/j.1439-0531.2010.01654\_3.x},
  volume       = {45},
  year         = {2010},
}

Chicago
Beek, Josine, Dominiek Maes, Hans Nauwynck, and Ann Van Soom. 2010. “Influence of Sperm-oocyte Coincubation Period on Porcine in Vitro Fertilization (IVF) Efficiency.” In Reproduction in Domestic Animals, 45:78–78. MALDEN: WILEY-BLACKWELL PUBLISHING, INC.
APA
Beek, J., Maes, D., Nauwynck, H., & Van Soom, A. (2010). Influence of sperm-oocyte coincubation period on porcine in vitro fertilization (IVF) efficiency. REPRODUCTION IN DOMESTIC ANIMALS (Vol. 45, pp. 78–78). Presented at the 14th Annual Conference of theEuropean-Societies-Domestic-Animals-Reproduction/22nd Annual Meeting of the EU-AI-Vets, MALDEN: WILEY-BLACKWELL PUBLISHING, INC.
Vancouver
1.
Beek J, Maes D, Nauwynck H, Van Soom A. Influence of sperm-oocyte coincubation period on porcine in vitro fertilization (IVF) efficiency. REPRODUCTION IN DOMESTIC ANIMALS. MALDEN: WILEY-BLACKWELL PUBLISHING, INC; 2010. p. 78–78.
MLA
Beek, Josine, Dominiek Maes, Hans Nauwynck, et al. “Influence of Sperm-oocyte Coincubation Period on Porcine in Vitro Fertilization (IVF) Efficiency.” Reproduction in Domestic Animals. Vol. 45. MALDEN: WILEY-BLACKWELL PUBLISHING, INC, 2010. 78–78. Print.