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TSOL18 vaccine of Taenia solium: development of monoclonal antibodies and field testing of the vaccine in Cameroon

Emmanuel Assana UGent (2010)
abstract
Chapter 1 reviews the literature about the immunological aspects of taeniid cestode infections and the existing vaccines against Taenia solium cysticercosis in pigs. One of the most promising vaccines is TSOL18, a protein that has been identified in the oncosphere of Taenia solium and expressed as a recombinant molecule in E. coli. Repeated experimental trials have shown that this vaccine is able to protect up to 100 % of the immunised pigs against a challenge infection with T. solium. Antibodies raised by the vaccine are capable of killing the parasite in in vitro cultures and it is believed that antibody and complement mediated killing of invading parasites is the major protective immune mechanism induced by vaccination with TSOL18. The identification of the villages with a high risk of T. solium infection, which could subsequently be used in the vaccine trial, is reported in chapter 2. A survey was conducted in 150 households owning 1756 pigs in the rural areas of Mayo-Danay division in the far north region of Cameroon. A questionnaire survey was carried out to collect information on the pig farming system and to identify potential risk factors for T. solium cysticercosis infection in pigs. Blood samples were collected from 398 pigs with the aim of estimating the sero-prevalence of Taenia solium cysticercosis. The results showed that 90.7 % of the pigs were free roaming during the dry season and that 42.7 % of households keeping pigs in the rural areas had no latrine facility. Seventy six percent of the interviewed pig owners affirmed that the members of the household used open field defecation. ELISA for antigen and antibody detection showed an apparent prevalence of porcine cysticercosis of 24.6 % and 32.2 %, respectively. A Bayesian approach using the conditional dependence between the two diagnostic tests indicated that the true seroprevalence of cysticercosis in Mayo-Danay was 26.6 %. Binary logistic regression analysis indicated that the lack of knowledge of the taeniasis-cysticercosis complex and the absence of a pig pen in the household were associated with pig cysticercosis.
Please use this url to cite or link to this publication:
author
promoter
UGent and Stanny Geerts
organization
year
type
dissertation (monograph)
subject
pages
VII, 141 pages
publisher
Ghent University. Faculty of Veterinary Medicine
place of publication
Merelbeke, Belgium
defense location
Merelbeke : Faculteit Diergeneeskunde (auditorium D)
defense date
2010-06-29 16:00
ISBN
9789058642295
language
English
UGent publication?
yes
classification
D1
additional info
dissertation consists of copyrighted materials
copyright statement
I have transferred the copyright for this publication to the publisher
id
1026199
handle
http://hdl.handle.net/1854/LU-1026199
date created
2010-08-19 15:55:56
date last changed
2010-11-17 09:51:35
@phdthesis{1026199,
  abstract     = {Chapter 1 reviews the literature about the immunological aspects of taeniid cestode infections and the existing vaccines against Taenia solium cysticercosis in pigs. One of the most promising vaccines is TSOL18, a protein that has been identified in the oncosphere of Taenia solium and expressed as a recombinant molecule in E. coli. Repeated experimental trials have shown that this vaccine is able to protect up to 100 \% of the immunised pigs against a challenge infection with T. solium. Antibodies raised by the vaccine are capable of killing the parasite in in vitro cultures and it is believed that antibody and complement mediated killing of invading parasites is the major protective immune mechanism induced by vaccination with TSOL18.
The identification of the villages with a high risk of T. solium infection, which could subsequently be used in the vaccine trial, is reported in chapter 2. A survey was conducted in 150 households owning 1756 pigs in the rural areas of Mayo-Danay division in the far north region of Cameroon. A questionnaire survey was carried out to collect information on the pig farming system and to identify potential risk factors for T. solium cysticercosis infection in pigs. Blood samples were collected from 398 pigs with the aim of estimating the sero-prevalence of Taenia solium cysticercosis. The results showed that 90.7 \% of the pigs were free roaming during the dry season and that 42.7 \% of households keeping pigs in the rural areas had no latrine facility.
Seventy six percent of the interviewed pig owners affirmed that the members of the household used open field defecation. ELISA for antigen and antibody detection showed an apparent prevalence of porcine cysticercosis of 24.6 \% and 32.2 \%, respectively. A Bayesian approach using the conditional dependence between the two diagnostic tests indicated that the true seroprevalence of cysticercosis in Mayo-Danay was 26.6 \%. Binary logistic regression analysis indicated that the lack of knowledge of the taeniasis-cysticercosis complex and the absence of a pig pen in the household were associated with pig cysticercosis.},
  author       = {Assana, Emmanuel},
  isbn         = {9789058642295},
  language     = {eng},
  pages        = {VII, 141},
  publisher    = {Ghent University. Faculty of Veterinary Medicine},
  school       = {Ghent University},
  title        = {TSOL18 vaccine of Taenia solium: development of monoclonal antibodies and field testing of the vaccine in Cameroon},
  year         = {2010},
}

Chicago
Assana, Emmanuel. 2010. “TSOL18 Vaccine of Taenia Solium: Development of Monoclonal Antibodies and Field Testing of the Vaccine in Cameroon”. Merelbeke, Belgium: Ghent University. Faculty of Veterinary Medicine.
APA
Assana, Emmanuel. (2010). TSOL18 vaccine of Taenia solium: development of monoclonal antibodies and field testing of the vaccine in Cameroon. Ghent University. Faculty of Veterinary Medicine, Merelbeke, Belgium.
Vancouver
1.
Assana E. TSOL18 vaccine of Taenia solium: development of monoclonal antibodies and field testing of the vaccine in Cameroon. [Merelbeke, Belgium]: Ghent University. Faculty of Veterinary Medicine; 2010.
MLA
Assana, Emmanuel. “TSOL18 Vaccine of Taenia Solium: Development of Monoclonal Antibodies and Field Testing of the Vaccine in Cameroon.” 2010 : n. pag. Print.