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Developing a safe antifungal treatment protocol to eliminate Batrachochytrium dendropbatidis from amphibians

An Martel UGent, Pascale Van Rooij UGent, Griet Vercauteren UGent, Kris Baert UGent, Lieven Van Waeyenberghe UGent, Patrick De Backer UGent, Trent WJ Garner, Tonnie Woeltjes, Richard Ducatelle UGent and Freddy Haesebrouck UGent, et al. (2011) MEDICAL MYCOLOGY. 49(2). p.143-149
abstract
Batrachochytrium dendrobatidis is one of the most pathogenic microorganisms affecting amphibians in both captivity and in nature. The establishment of B. dendrobatidis free, stable, amphibian captive breeding colonies is one of the emergency measures that is being taken to save threatened amphibian species from extinction. For this purpose, in vitro antifungal susceptibility testing and the development of efficient and safe treatment protocols are required. In this study, we evaluated the use of amphotericin B and voriconazole to treat chytridiomycosis in amphibians. The concentration at which the growth of five tested B. dendrobatidis strains was inhibited was 0.8 µg/ml for amphotericin B and 0.0125 µg/ml for voriconazole. To completely eliminate a mixture of sporangia and zoospores of strain IA042 required 48 h of exposure to 8 µg/ml of amphotericin B or 10 days to 1.25 µg/ml of voriconazole. Zoospores were killed within 0.5 h by 0.8 µg/ml of amphotericin B, but even after 24 h exposure to 1.25 µg/ml of voriconazole they remained viable. Amphotericin B was acutely toxic for Alytes muletensis tadpoles at 8 µg/ml, whereas toxic side effects were not noticed during a seven-day exposure to voriconazole at concentrations as high as 12.5 µg/ml. The voriconazole concentrations remained stable in water during this exposure period. On the basis of this data, experimentally inoculated postmetamorphic Alytes cisternasii were sprayed once daily for 7 days with a 1.25 µg/ml solution of voriconazole in water which eliminated the B. dendrobatidis infection from all treated animals. Finally, treatment of a naturally infected colony of poison dart frogs (Dendrobatidae) using this protocol, combined with environmental disinfection, cleared the infection from the colony.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Batrachochytrium dendrobatidis, voriconazole, amphibian, treatment, CHYTRID FUNGUS, CHYTRIDIOMYCOSIS, INFECTION, DISEASE, VORICONAZOLE, STABILITY, DIAGNOSIS, FROGS
journal title
MEDICAL MYCOLOGY
Med. Mycol.
volume
49
issue
2
pages
143 - 149
Web of Science type
Article
Web of Science id
000287451500005
JCR category
VETERINARY SCIENCES
JCR impact factor
2.457 (2011)
JCR rank
5/141 (2011)
JCR quartile
1 (2011)
ISSN
1369-3786
DOI
10.3109/13693786.2010.508185
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1025818
handle
http://hdl.handle.net/1854/LU-1025818
date created
2010-08-18 17:30:10
date last changed
2011-05-18 13:47:37
@article{1025818,
  abstract     = {Batrachochytrium dendrobatidis is one of the most pathogenic microorganisms affecting amphibians in both captivity and in nature. The establishment of B. dendrobatidis  free, stable, amphibian captive breeding colonies is one of the emergency measures that is being taken to save threatened amphibian species from extinction. For this purpose, in vitro antifungal susceptibility testing and the development of efficient and safe treatment protocols are required. In this study, we evaluated the use of amphotericin B and voriconazole to treat chytridiomycosis in amphibians. The concentration at which the growth of five tested B. dendrobatidis  strains was inhibited was 0.8 {\textmu}g/ml for amphotericin B and 0.0125 {\textmu}g/ml for voriconazole. To completely eliminate a mixture of sporangia and zoospores of strain IA042 required 48 h of exposure to 8 {\textmu}g/ml of amphotericin B or 10 days to 1.25 {\textmu}g/ml of voriconazole. Zoospores were killed within 0.5 h by 0.8 {\textmu}g/ml of amphotericin B, but even after 24 h exposure to 1.25 {\textmu}g/ml of voriconazole they remained viable. Amphotericin B was acutely toxic for Alytes muletensis tadpoles at 8 {\textmu}g/ml, whereas toxic side effects were not noticed during a seven-day exposure to voriconazole at concentrations as high as 12.5 {\textmu}g/ml. The voriconazole concentrations remained stable in water during this exposure period. On the basis of this data, experimentally inoculated postmetamorphic Alytes cisternasii were sprayed once daily for 7 days with a 1.25 {\textmu}g/ml solution of voriconazole in water which eliminated the B. dendrobatidis  infection from all treated animals. Finally, treatment of a naturally infected colony of poison dart frogs (Dendrobatidae) using this protocol, combined with environmental disinfection, cleared the infection from the colony.},
  author       = {Martel, An and Van Rooij, Pascale and Vercauteren, Griet and Baert, Kris and Van Waeyenberghe, Lieven and De Backer, Patrick and Garner, Trent WJ and Woeltjes, Tonnie and Ducatelle, Richard and Haesebrouck, Freddy and Pasmans, Frank},
  issn         = {1369-3786},
  journal      = {MEDICAL MYCOLOGY},
  keyword      = {Batrachochytrium dendrobatidis,voriconazole,amphibian,treatment,CHYTRID FUNGUS,CHYTRIDIOMYCOSIS,INFECTION,DISEASE,VORICONAZOLE,STABILITY,DIAGNOSIS,FROGS},
  language     = {eng},
  number       = {2},
  pages        = {143--149},
  title        = {Developing a safe antifungal treatment protocol to eliminate Batrachochytrium dendropbatidis from amphibians},
  url          = {http://dx.doi.org/10.3109/13693786.2010.508185},
  volume       = {49},
  year         = {2011},
}

Chicago
Martel, An, Pascale Van Rooij, Griet Vercauteren, Kris Baert, Lieven Van Waeyenberghe, Patrick De Backer, Trent WJ Garner, et al. 2011. “Developing a Safe Antifungal Treatment Protocol to Eliminate Batrachochytrium Dendropbatidis from Amphibians.” Medical Mycology 49 (2): 143–149.
APA
Martel, A., Van Rooij, P., Vercauteren, G., Baert, K., Van Waeyenberghe, L., De Backer, P., Garner, T. W., et al. (2011). Developing a safe antifungal treatment protocol to eliminate Batrachochytrium dendropbatidis from amphibians. MEDICAL MYCOLOGY, 49(2), 143–149.
Vancouver
1.
Martel A, Van Rooij P, Vercauteren G, Baert K, Van Waeyenberghe L, De Backer P, et al. Developing a safe antifungal treatment protocol to eliminate Batrachochytrium dendropbatidis from amphibians. MEDICAL MYCOLOGY. 2011;49(2):143–9.
MLA
Martel, An, Pascale Van Rooij, Griet Vercauteren, et al. “Developing a Safe Antifungal Treatment Protocol to Eliminate Batrachochytrium Dendropbatidis from Amphibians.” MEDICAL MYCOLOGY 49.2 (2011): 143–149. Print.