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Binding of manno-oligosaccharides to concanavalin A: substitution titration with a fluorescent-indicator ligand

(1980) EUROPEAN JOURNAL OF BIOCHEMISTRY. 103(2). p.307-312
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Abstract
The association constants for binding of methyl alpha-D-mannopyranoside (I), mannobiose (II) and mannotriose (III) to concanavalin A were determined in the temperature range 285-313 °K by a substitution titration, using 4-methylumbelliferyl alpha-D-mannopyranoside as a carbohydrate-specific and fluorescent indicator. All binding equilibria are simple, but establish extremely slowly with II and III. At 298.3 °K, K increases moderately from I to III: (6.4 +/- 0.5) x 10(3), (1.2 +/- 0.1) x 10(4) and (1.10 +/- 0.05) x 10(5) M-1. For binding of I, II and III, the - delta H degree values are constant (36 +/- 2 kJ mol-1) and equal to the average value (36.1 +/- 0.6 kJ mol-1) obtained for the three corresponding 4-methylumbelliferyl alpha-D-mannooligosaccharides [Van Landschoot, A., Loontiens, F. G., and De Bruyne, C. K (1978) Eur. J. Biochem. 83, 277-285]. The data are interpreted as arising from specific binding to a single mannopyranosyl residue in (alpha 1 leads to 2)-linked mannooligosaccharides.
Keywords
Concanavalin A, mannooligosaccharides, fluorescence

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Chicago
Van Landschoot, Anita, Frank Loontiens, and Clemens De Bruyne. 1980. “Binding of Manno-oligosaccharides to Concanavalin A: Substitution Titration with a Fluorescent-indicator Ligand.” European Journal of Biochemistry 103 (2): 307–312.
APA
Van Landschoot, Anita, Loontiens, F., & De Bruyne, C. (1980). Binding of manno-oligosaccharides to concanavalin A: substitution titration with a fluorescent-indicator ligand. EUROPEAN JOURNAL OF BIOCHEMISTRY, 103(2), 307–312.
Vancouver
1.
Van Landschoot A, Loontiens F, De Bruyne C. Binding of manno-oligosaccharides to concanavalin A: substitution titration with a fluorescent-indicator ligand. EUROPEAN JOURNAL OF BIOCHEMISTRY. 1980;103(2):307–12.
MLA
Van Landschoot, Anita, Frank Loontiens, and Clemens De Bruyne. “Binding of Manno-oligosaccharides to Concanavalin A: Substitution Titration with a Fluorescent-indicator Ligand.” EUROPEAN JOURNAL OF BIOCHEMISTRY 103.2 (1980): 307–312. Print.
@article{1023657,
  abstract     = {The association constants for binding of methyl alpha-D-mannopyranoside (I), mannobiose (II) and mannotriose (III) to concanavalin A were determined in the temperature range 285-313 {\textdegree}K by a substitution titration, using 4-methylumbelliferyl alpha-D-mannopyranoside as a carbohydrate-specific and fluorescent indicator. All binding equilibria are simple, but establish extremely slowly with II and III. At 298.3 {\textdegree}K, K increases moderately from I to III: (6.4 +/- 0.5) x 10(3), (1.2 +/- 0.1) x 10(4) and (1.10 +/- 0.05) x 10(5) M-1. For binding of I, II and III, the - delta H degree values are constant (36 +/- 2 kJ mol-1) and equal to the average value (36.1 +/- 0.6 kJ mol-1) obtained for the three corresponding 4-methylumbelliferyl alpha-D-mannooligosaccharides [Van Landschoot, A., Loontiens, F. G., and De Bruyne, C. K (1978) Eur. J. Biochem. 83, 277-285]. The data are interpreted as arising from specific binding to a single mannopyranosyl residue in (alpha 1 leads to 2)-linked mannooligosaccharides.},
  author       = {Van Landschoot, Anita and Loontiens, Frank and De Bruyne, Clemens},
  issn         = {0014-2956},
  journal      = {EUROPEAN JOURNAL OF BIOCHEMISTRY},
  keyword      = {Concanavalin A,mannooligosaccharides,fluorescence},
  language     = {eng},
  number       = {2},
  pages        = {307--312},
  title        = {Binding of manno-oligosaccharides to concanavalin A: substitution titration with a fluorescent-indicator ligand},
  url          = {http://dx.doi.org/10.1111/j.1432-1033.1980.tb04316.x},
  volume       = {103},
  year         = {1980},
}

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