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On the production and identification of medium-chained sophorolipids

Steve JJ Fleurackers, Inge Van Bogaert UGent and Dirk Develter (2010) EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY. 112(6). p.655-662
abstract
Sophorolipids are biologically produced surfactants that consist of a hydrophilic sophorose moiety bound to a hydroxylated lipophilic substrate. The highest levels of production are currently achieved using Candida bombicola ATCC 22214, an ascomycotous yeast, as the production organism. The lipophilic moiety is typically found to have a length between C-16 and C-18 and thus the applications of readily produced sophorolipids with regard to cleaning purposes are somewhat limited. Experiments were performed to evaluate the conditions under which a beta-oxidation deficient strain of C. bombicola is able to convert 1-dodecanol into sophorolipids. Both shakeflask and bioreactor cultures yielded C-12, or medium-chained, sophorolipids and the effect of aeration on the production level was investigated and found to favour limited aeration. Analysis of the conversion products was performed through RP-HPLC and the formation of new-to-nature sophorolipids was confirmed by MS. The use of the beta-oxidation deficient strain under limited aeration yielded at least 15 g/L of medium-chained sophorolipids, which is more than in the wild-type strain or when employing full aeration.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
beta-Oxidation, Candida bombicola, Genetic engineering, Biosurfactants, 1-Dodecanol, SOPHOROSE LIPIDS, MICROBIAL-PRODUCTION, CANDIDA-BOMBICOLA, SURFACTANTS, OIL
journal title
EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY
Eur. J. Lipid Sci. Technol.
volume
112
issue
6
pages
655 - 662
Web of Science type
Article
Web of Science id
000279978600005
JCR category
FOOD SCIENCE & TECHNOLOGY
JCR impact factor
1.487 (2010)
JCR rank
44/124 (2010)
JCR quartile
2 (2010)
ISSN
1438-7697
DOI
10.1002/ejlt.200900158
language
English
UGent publication?
no
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
1023421
handle
http://hdl.handle.net/1854/LU-1023421
date created
2010-08-15 13:41:10
date last changed
2010-08-20 09:17:05
@article{1023421,
  abstract     = {Sophorolipids are biologically produced surfactants that consist of a hydrophilic sophorose moiety bound to a hydroxylated lipophilic substrate. The highest levels of production are currently achieved using Candida bombicola ATCC 22214, an ascomycotous yeast, as the production organism. The lipophilic moiety is typically found to have a length between C-16 and C-18 and thus the applications of readily produced sophorolipids with regard to cleaning purposes are somewhat limited. Experiments were performed to evaluate the conditions under which a beta-oxidation deficient strain of C. bombicola is able to convert 1-dodecanol into sophorolipids. Both shakeflask and bioreactor cultures yielded C-12, or medium-chained, sophorolipids and the effect of aeration on the production level was investigated and found to favour limited aeration. Analysis of the conversion products was performed through RP-HPLC and the formation of new-to-nature sophorolipids was confirmed by MS. The use of the beta-oxidation deficient strain under limited aeration yielded at least 15 g/L of medium-chained sophorolipids, which is more than in the wild-type strain or when employing full aeration.},
  author       = {Fleurackers, Steve JJ  and Van Bogaert, Inge and Develter, Dirk},
  issn         = {1438-7697},
  journal      = {EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY},
  keyword      = {beta-Oxidation,Candida bombicola,Genetic engineering,Biosurfactants,1-Dodecanol,SOPHOROSE LIPIDS,MICROBIAL-PRODUCTION,CANDIDA-BOMBICOLA,SURFACTANTS,OIL},
  language     = {eng},
  number       = {6},
  pages        = {655--662},
  title        = {On the production and identification of medium-chained sophorolipids},
  url          = {http://dx.doi.org/10.1002/ejlt.200900158},
  volume       = {112},
  year         = {2010},
}

Chicago
Fleurackers, Steve JJ , Inge Van Bogaert, and Dirk Develter. 2010. “On the Production and Identification of Medium-chained Sophorolipids.” European Journal of Lipid Science and Technology 112 (6): 655–662.
APA
Fleurackers, S. J., Van Bogaert, I., & Develter, D. (2010). On the production and identification of medium-chained sophorolipids. EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY, 112(6), 655–662.
Vancouver
1.
Fleurackers SJ, Van Bogaert I, Develter D. On the production and identification of medium-chained sophorolipids. EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY. 2010;112(6):655–62.
MLA
Fleurackers, Steve JJ , Inge Van Bogaert, and Dirk Develter. “On the Production and Identification of Medium-chained Sophorolipids.” EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY 112.6 (2010): 655–662. Print.