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Complementary positional proteomics for screening substrates of endo- and exoproteases

(2010) NATURE METHODS. 7(7). p.512-515
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Abstract
We describe a positional proteomics approach to simultaneously analyze N- and C-terminal peptides and used it to screen for human protein substrates of granzyme B and carboxypeptidase A4 in human cell lysates. This approach allowed comprehensive proteome studies, and we report the identification of 965 database-annotated protein C termini, 334 neo-C termini resulting from granzyme B processing and 16 neo-C termini resulting from carboxypeptidase A4 processing.
Keywords
PEPTIDES, TERMINI, GRANZYME-B, EXPRESSION, IDENTIFICATION, PROTEINS

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Chicago
Van Damme, Petra, An Staes, Silvia Bronsoms, Kenny Helsens, Niklaas Colaert, Evy Timmerman, Francesc X Aviles, Joël Vandekerckhove, and Kris Gevaert. 2010. “Complementary Positional Proteomics for Screening Substrates of Endo- and Exoproteases.” Nature Methods 7 (7): 512–515.
APA
Van Damme, Petra, Staes, A., Bronsoms, S., Helsens, K., Colaert, N., Timmerman, E., Aviles, F. X., et al. (2010). Complementary positional proteomics for screening substrates of endo- and exoproteases. NATURE METHODS, 7(7), 512–515.
Vancouver
1.
Van Damme P, Staes A, Bronsoms S, Helsens K, Colaert N, Timmerman E, et al. Complementary positional proteomics for screening substrates of endo- and exoproteases. NATURE METHODS. 2010;7(7):512–5.
MLA
Van Damme, Petra, An Staes, Silvia Bronsoms, et al. “Complementary Positional Proteomics for Screening Substrates of Endo- and Exoproteases.” NATURE METHODS 7.7 (2010): 512–515. Print.
@article{1021546,
  abstract     = {We describe a positional proteomics approach to simultaneously analyze N- and C-terminal peptides and used it to screen for human protein substrates of granzyme B and carboxypeptidase A4 in human cell lysates. This approach allowed comprehensive proteome studies, and we report the identification of 965 database-annotated protein C termini, 334 neo-C termini resulting from granzyme B processing and 16 neo-C termini resulting from carboxypeptidase A4 processing.},
  author       = {Van Damme, Petra and Staes, An and Bronsoms, Silvia and Helsens, Kenny and Colaert, Niklaas and Timmerman, Evy and Aviles, Francesc X and Vandekerckhove, Jo{\"e}l and Gevaert, Kris},
  issn         = {1548-7091},
  journal      = {NATURE METHODS},
  keyword      = {PEPTIDES,TERMINI,GRANZYME-B,EXPRESSION,IDENTIFICATION,PROTEINS},
  language     = {eng},
  number       = {7},
  pages        = {512--515},
  title        = {Complementary positional proteomics for screening substrates of endo- and exoproteases},
  url          = {http://dx.doi.org/10.1038/NMETH.1469},
  volume       = {7},
  year         = {2010},
}

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