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Identification and expression analysis of splice variants of mouse enabled homologue during development and in adult tissues

Sylvie Veniere UGent, Davy Waterschoot UGent, Joël Vandekerckhove UGent, Anja Lambrechts UGent and Christophe Ampe UGent (2010) BMC MOLECULAR BIOLOGY. 11.
abstract
Background: The Enabled/Vasodilator stimulated phosphoprotein (Ena/VASP) gene family comprises three genes in vertebrates: Vasp, Enabled homologue (Enah) and Ena-VASP like (Evl). Enah has the most complex gene structure. It has extra alternatively included exons compared to Vasp and Evl, and possibly one alternatively excluded intron S. The aim of this mapping study was to probe the occurrence of combinations of exon usage in Enah thereby identifying possible vertebrate ENAH splice variants. We investigated this via an in silico analysis and by performing a reverse transcription-polymerase chain reaction (RT-PCR) screen on mouse samples. We further probed the expression pattern of mouse Enah splice variants during development and in a selection of mouse adult tissues and mouse cell lines. Results: In silico analysis of the vertebrate Ena/VASP gene family reveals that birds do not have Vasp, while fish have two Evl genes. Analysis of expressed sequence tags of vertebrate Enah splice variants confirms that an Enah transcript without alternative exons is ubiquitously expressed, but yields only limited information about the existence of other possible alternatively spliced Enah transcripts. Via a RT-PCR screen, we provide evidence that during mouse development and in adult mice at least eight and maximally sixteen different Enah transcripts are expressed. We also show that tissues and cell lines display specific expression profiles of these different transcripts. Exons previously associated with neuronal expression of Enah splice variants are also present in other tissues, in particular in heart. Conclusions: We propose a more uniform nomenclature for alternative exons in Enah. We provide an overview of distinct expression profiles of mouse Enah splice variants during mouse development, in adult mouse tissues and in a subset of mouse cell lines.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
VASP, ACTIN, PHOSPHORYLATION, INVASION, LOCALIZATION, MENA, IN-VIVO, ENA/VASP PROTEINS, VASODILATOR-STIMULATED PHOSPHOPROTEIN, LOCALIZATION, CANCER CELL-LINES
journal title
BMC MOLECULAR BIOLOGY
BMC Mol. Biol.
volume
11
article_number
45
pages
13 pages
Web of Science type
Article
Web of Science id
000279932000001
JCR category
BIOCHEMISTRY & MOLECULAR BIOLOGY
JCR impact factor
3.188 (2010)
JCR rank
122/284 (2010)
JCR quartile
2 (2010)
ISSN
1471-2199
DOI
10.1186/1471-2199-11-45
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
1021517
handle
http://hdl.handle.net/1854/LU-1021517
date created
2010-08-12 11:08:39
date last changed
2013-01-30 09:41:36
@article{1021517,
  abstract     = {Background: The Enabled/Vasodilator stimulated phosphoprotein (Ena/VASP) gene family comprises three genes in vertebrates: Vasp, Enabled homologue (Enah) and Ena-VASP like (Evl). Enah has the most complex gene structure. It has extra alternatively included exons compared to Vasp and Evl, and possibly one alternatively excluded intron S. The aim of this mapping study was to probe the occurrence of combinations of exon usage in Enah thereby identifying possible vertebrate ENAH splice variants. We investigated this via an in silico analysis and by performing a reverse transcription-polymerase chain reaction (RT-PCR) screen on mouse samples. We further probed the expression pattern of mouse Enah splice variants during development and in a selection of mouse adult tissues and mouse cell lines.
Results: In silico analysis of the vertebrate Ena/VASP gene family reveals that birds do not have Vasp, while fish have two Evl genes. Analysis of expressed sequence tags of vertebrate Enah splice variants confirms that an Enah transcript without alternative exons is ubiquitously expressed, but yields only limited information about the existence of other possible alternatively spliced Enah transcripts. Via a RT-PCR screen, we provide evidence that during mouse development and in adult mice at least eight and maximally sixteen different Enah transcripts are expressed. We also show that tissues and cell lines display specific expression profiles of these different transcripts. Exons previously associated with neuronal expression of Enah splice variants are also present in other tissues, in particular in heart.
Conclusions: We propose a more uniform nomenclature for alternative exons in Enah. We provide an overview of distinct expression profiles of mouse Enah splice variants during mouse development, in adult mouse tissues and in a subset of mouse cell lines.},
  articleno    = {45},
  author       = {Veniere, Sylvie and Waterschoot, Davy and Vandekerckhove, Jo{\"e}l and Lambrechts, Anja and Ampe, Christophe},
  issn         = {1471-2199},
  journal      = {BMC MOLECULAR BIOLOGY},
  keyword      = {VASP,ACTIN,PHOSPHORYLATION,INVASION,LOCALIZATION,MENA,IN-VIVO,ENA/VASP PROTEINS,VASODILATOR-STIMULATED PHOSPHOPROTEIN,LOCALIZATION,CANCER CELL-LINES},
  language     = {eng},
  pages        = {13},
  title        = {Identification and expression analysis of splice variants of mouse enabled homologue during development and in adult tissues},
  url          = {http://dx.doi.org/10.1186/1471-2199-11-45},
  volume       = {11},
  year         = {2010},
}

Chicago
Veniere, Sylvie, Davy Waterschoot, Joël Vandekerckhove, Anja Lambrechts, and Christophe Ampe. 2010. “Identification and Expression Analysis of Splice Variants of Mouse Enabled Homologue During Development and in Adult Tissues.” Bmc Molecular Biology 11.
APA
Veniere, S., Waterschoot, D., Vandekerckhove, J., Lambrechts, A., & Ampe, C. (2010). Identification and expression analysis of splice variants of mouse enabled homologue during development and in adult tissues. BMC MOLECULAR BIOLOGY, 11.
Vancouver
1.
Veniere S, Waterschoot D, Vandekerckhove J, Lambrechts A, Ampe C. Identification and expression analysis of splice variants of mouse enabled homologue during development and in adult tissues. BMC MOLECULAR BIOLOGY. 2010;11.
MLA
Veniere, Sylvie, Davy Waterschoot, Joël Vandekerckhove, et al. “Identification and Expression Analysis of Splice Variants of Mouse Enabled Homologue During Development and in Adult Tissues.” BMC MOLECULAR BIOLOGY 11 (2010): n. pag. Print.