Advanced search
1 file | 240.22 KB Add to list

Raman spectra of pure biomolecules obtained using a handheld instrument under cold high-altitude conditions

Author
Organization
Abstract
A handheld Raman spectrometer (Ahura First Defender) was tested for the unambiguous identification of biomolecules (pure amino acids, carboxylic acids, saccharides and trehalose) in the solid state under outdoor conditions (including moderate climate conditions as well as cold temperatures and high altitudes). The biomolecules investigated represent important objects of interest for future exobiological missions. Repetitive measurements carried out under identical instrumental setups confirmed the excellent reliability of the Raman spectrometer. Raman bands are found at correct wavenumbers +/-3 cm(-1) compared with reference values. This testing represents the first step in a series of studies. In a preliminary, challenging investigation to determine the detection limit for glycine dispersed in a powdered gypsum matrix, 10% was the lowest content confirmed unambiguously. Clearly there is a need to investigate further the detection limits of Raman spectroscopic analyses of biomolecules in more complex samples, to demonstrate the usefulness or disqualify the use of this technique for more realistic outdoor situations, such as eventual future missions to Mars.
Keywords
EXTRACTING BIOCHEMICAL INFORMATION, Amino acids, PHOENIX LANDING SITE, Exobiology, Low-temperature conditions, In situ examination, Handheld instrumentation, Raman spectroscopy, TOOL, EXPLORATION, SPECTROMETER, MARS, DEEP-OCEAN, SPECTROSCOPY, MINERALS, REFERENCE DATABASE

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 240.22 KB

Citation

Please use this url to cite or link to this publication:

MLA
Jehlička, Jan, Peter Vandenabeele, Howell GM Edwards, et al. “Raman Spectra of Pure Biomolecules Obtained Using a Handheld Instrument Under Cold High-altitude Conditions.” ANALYTICAL AND BIOANALYTICAL CHEMISTRY 397.7 (2010): 2753–2760. Print.
APA
Jehlička, J., Vandenabeele, P., Edwards, H. G., Culka, A., & Čapoun, T. (2010). Raman spectra of pure biomolecules obtained using a handheld instrument under cold high-altitude conditions. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 397(7), 2753–2760.
Chicago author-date
Jehlička, Jan, Peter Vandenabeele, Howell GM Edwards, Adam Culka, and Tomáš Čapoun. 2010. “Raman Spectra of Pure Biomolecules Obtained Using a Handheld Instrument Under Cold High-altitude Conditions.” Analytical and Bioanalytical Chemistry 397 (7): 2753–2760.
Chicago author-date (all authors)
Jehlička, Jan, Peter Vandenabeele, Howell GM Edwards, Adam Culka, and Tomáš Čapoun. 2010. “Raman Spectra of Pure Biomolecules Obtained Using a Handheld Instrument Under Cold High-altitude Conditions.” Analytical and Bioanalytical Chemistry 397 (7): 2753–2760.
Vancouver
1.
Jehlička J, Vandenabeele P, Edwards HG, Culka A, Čapoun T. Raman spectra of pure biomolecules obtained using a handheld instrument under cold high-altitude conditions. ANALYTICAL AND BIOANALYTICAL CHEMISTRY. 2010;397(7):2753–60.
IEEE
[1]
J. Jehlička, P. Vandenabeele, H. G. Edwards, A. Culka, and T. Čapoun, “Raman spectra of pure biomolecules obtained using a handheld instrument under cold high-altitude conditions,” ANALYTICAL AND BIOANALYTICAL CHEMISTRY, vol. 397, no. 7, pp. 2753–2760, 2010.
@article{1020617,
  abstract     = {A handheld Raman spectrometer (Ahura First Defender) was tested for the unambiguous identification of biomolecules (pure amino acids, carboxylic acids, saccharides and trehalose) in the solid state under outdoor conditions (including moderate climate conditions as well as cold temperatures and high altitudes). The biomolecules investigated represent important objects of interest for future exobiological missions. Repetitive measurements carried out under identical instrumental setups confirmed the excellent reliability of the Raman spectrometer. Raman bands are found at correct wavenumbers +/-3 cm(-1) compared with reference values. This testing represents the first step in a series of studies. In a preliminary, challenging investigation to determine the detection limit for glycine dispersed in a powdered gypsum matrix, 10% was the lowest content confirmed unambiguously. Clearly there is a need to investigate further the detection limits of Raman spectroscopic analyses of biomolecules in more complex samples, to demonstrate the usefulness or disqualify the use of this technique for more realistic outdoor situations, such as eventual future missions to Mars.},
  author       = {Jehlička, Jan and Vandenabeele, Peter and Edwards, Howell GM and Culka, Adam and Čapoun, Tomáš},
  issn         = {1618-2642},
  journal      = {ANALYTICAL AND BIOANALYTICAL CHEMISTRY},
  keywords     = {EXTRACTING BIOCHEMICAL INFORMATION,Amino acids,PHOENIX LANDING SITE,Exobiology,Low-temperature conditions,In situ examination,Handheld instrumentation,Raman spectroscopy,TOOL,EXPLORATION,SPECTROMETER,MARS,DEEP-OCEAN,SPECTROSCOPY,MINERALS,REFERENCE DATABASE},
  language     = {eng},
  number       = {7},
  pages        = {2753--2760},
  title        = {Raman spectra of pure biomolecules obtained using a handheld instrument under cold high-altitude conditions},
  url          = {http://dx.doi.org/10.1007/s00216-010-3849-7},
  volume       = {397},
  year         = {2010},
}

Altmetric
View in Altmetric
Web of Science
Times cited: